Oligonucleotide Synthesis Core Facility

Animal Clinical Chemistry

Mission: The Animal Clinical Laboratory Core Facility can assist research investigators who would greatly benefit from availability of service (on a cost-per-test basis) that replicates for animals the laboratory tests that are routine for humans. Examples include blood chemistry, urinalysis, and hematology. This core facility has been providing services for the UNC campus since 1997.

Services: We provide four different types of service for clinical chemistry:

The core also provides consultation and training in the application of these services.

1) Clinical Tests.Each individual test requires 10 ul plasma, serum, or urine. An extra 10 ul sample per test is needed for machine dead volume. For examples, when user wants four different chemical tests from a sample, user should bring that sample with at least 60 µl. Available tests are listed below:

a) Ions:

Ammonia, Blood Urea Nitrogen, Ca, Cl, CO₂, Fe, Total Iron-Binding Capacity, K, Lithium, Mg, Na, and Phosphorus

b) Drugs:

Acetaminophen, Alcohol, Carbamazepine, Digoxin, Theophylline, Phenytoin, and Salicylate

c) Proteins and Organic Chemicals:

Albumin, Conjugated and Unconjugated Bilirubin, Total Bilirubin, Creatinine, Cholesterol, HDL-Cholesterol, Glucose, Total Protein, C-Reactive Protein, Urine Protein, Cerebral Spinal Fluid Protein, Triglycerides, Uric Acid, and Urine Creatinine

d) Enzymes:

Alanine Aminotransferase, Acid Phosphatase, Alkaline Phosphatase, Amylase, Aspartate Aminotransferase, Cholinesterase, Creatinine Kinase MB, GGT, Lactate, LDH, and Lipase

2) Hematological Tests. These tests require 25 μl EDTA-whole blood per sample. The values analyzed are as follows:

WBC#, Lym%, Lym#, Mon%, Mon#, Gra%, Gra#, RBC#, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV, and 3 distribution curves: WBC, RBC, and PLT

3) Group Tests. These include the following:

a) General Health Test:

Hematological Test, Albumin, Blood Urea Nitrogen, Creatinine, and Glucose

b) Kidney Test:

Blood Urea Nitrogen, Creatinine, Na, K, and Cl

c) Liver Test:

Albumin, Alkaline Phosphatase, Alanine Aminotransferase, and Aspartate Aminotransferase

4) Multiplexed Biomarker Immunoassays.

We now offer multiplexed biomarker immunoassays for Cytokine/Chemokine detection, metabolism, toxicity, cancer biomarkers, and many other disease states by using the Luminex technology. The new Luminex MAGPIX system using magnetic bead-based multi-analytes provides a complete solution for rapid, accurate biomarker quantitation in a variety of sample matrices. This affordable system can perform up to 50 tests simultaneously in a single reaction volume, greatly reducing sample input (10-20ul/sample), reagents and labor while improving productivity. The MILLIPLEX magnetic bead-based multi-analyte panels from EMD Millipore Company (see below kits) enable researchers to gain more information faster without compromising reliability. Furthermore, an automated microplate washer from BioTek Company can enhance magnetic bead assays by complete plate biomagnetic separation during washing

Luminex MAGPIX system services and costs;

a) Full service covers whole procedures, ordering kits, performing experiments, analyzing data, and sending results (customers can only provide samples).

Cost; A Milliplex kit, 96 well formate for standard curves and controls (20 wells) and 76 unknown samples (~$200/analyte with current 25% discount, if 5 multiplexed it costs ~$1,000).

And plus research technician working hrs, ~8 hrs ($200).

b) If you do your own assays you must do the following:

Order your own reaction kits

Do your own experiments

$25 per machine use

$40 consultant and training (one time)

c) Available kits: (MAGPIX Millipex, June 2011)

For mouse samples:

Metabolism

1) Metabolic Hormone (Cat# MMHMAG-44K)

IL-6, Amylin (active), C-Peptide 2, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, Insulin,

Leptin, MCP-1, Pancreatic Polypeptide, PYY (total), Resistin, TNFa

2) Multi Species Steroid/Thyroid Hormone ( Cat# STTHMAG-21K)

Cortisol, Estradiol, Progesterone, T3, T4

3) IGF Binding Protein (Cat# MIGFBPMAG-43K)

IGFBP1, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IGFBP7

Inflammation/Immunology

4) Cytokine/Chemokine Panel I (Cat# MCYTOMAG-70K)

IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17a, Eotaxin,

G-CSF, GM-CSF, IFNg, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1a, MIP-1b, MIP-2, RANTES, TNFa, VEGF

5) Cytokine/Chemokine Panel II (Cat# MCYP2MAG)

IL-16, IL-21, IL-22, IL-25/IL-17, IL-28B, EPO, Exodus-2, Fractalkine, MCP-5, MIP-3a, MIP-3b, TARC

6) Cytokine/Chemokine Panel III (Cat# MCYP3MAG)

IL-20, IL-23, Il-27, IL-33, MDC, TIMP-1

For human samples:

Metabolism

1) Metabolic Hormone ( Cat# HMHMAG-34K)

IL-6, Amylin (active), C-Peptide 2, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, Insulin,

Leptin, MCP-1, Pancreatic Polypeptide, PYY (total), Resistin, TNFa

2) Liver Protein (Cat# HLPPMAG-57K)

AFP, ANGPTL3, ANGPTL4, ANGPTL6, FABP1, FGF-19, FGF-21, FGF-23, HGF

3) Multi Species Steroid/Thyroid Hormone ( Cat# STTHMAG-21K)

Cortisol, Estradiol, Progesterone, T3, T4

Neuroscience

4) Neurological Disorders Panel 2 (Cat# HND2MAG-39K)

AGP, Ceruloplasmin, Haptoglobin, SAP

Toxicity

5) Kidney Toxicity Panel 1 (Cat# HKTX1MAG-38K)

KIM-1, Osteopontin, Renin, TFF-3

6) Kidney Toxicity Panel 2 ( Cat# HKTX2MAG-38K)

B2-Microglobulin, Clusterin, Cystatin C

7) Kidney Toxicity Panel 3 (Cat# HKYX3MAG-38K)

KIM-1, Renin, TFF-3

8) Kidney Toxicity Panel 4 ( Cat# HKTX4MAG-38K)

Albumin, B2-Microglobulin, Clusterin, Cystatin C, Osteopontin

Cancer Biomarker

9) Circulating Cancer Biomarker Panel 1 (Cat# HCCBP1MAG-58K)

IL-6, IL-8, AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, FGF2/bFGF, HCGb, HE4, HGF, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, SDF-1a+b/CXCL12, sFas, sFasL, TGFa, TNFa, TRAIL,

VEGF

10) Circulating Cancer Biomarker Panel 2 (Cat# HND2MAG-39K)

Antithrombin III, Coagulation factor XIII, Complement factor H (CFH), Extracellular matrix protein 1 (ECM1), Vitamin D binding protein, Vitronectin

Inflammation/Immunology

11) Cytokine/Chemokine Panel I(Cat# MCYTOMAG-70K)

IL-1a, IL-1b, IL-1ra, IL-2, sIL-2Ra, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13,

IL-15, IL-17a, sCD40L, EGF, Eotaxin, FGF-2, Fit-3 ligand, Fractakine, G-CSF, GM-CSF, GRO, IFNa2, IFNg,

IP-10, MCP-1, MCP-3/CCL7, MDC/CCL22, MIP-1a, MIP-1b, PDGF-AA, PDGF-AB/BB, RANTES, TGFa, TNFa,

TNFb, VEGF

12) Cytokine/Chemokine Panel II

IL-16, IL-20, IL-21, Il-23, IL-28a, IL-33/NF-HEV (mature), 6CKine/CCL21, BCA-1/CXCL13, CTACK/CCL27, ENA-78/CXCL5, Eotaxin-2/CCL24/MPIF-2, Eotaxin-3/CCL26, I-309/CCL1, LIF, MCP-2, MCP-4,

MIP-1d (MIP-5/CCL15), SCF, SDF-1a+b/CXCL12, TARC/CCL17, TPO, TRAIL/TNFSF10, TSLP

13) Cytokine/Chemokine Panel III (Cat# HCYP3MAG-63K)

IL-11, IL-29/IFNg2, GCP-2/CXCL6/LIX, HCC-1/CCL14a, I-TAC/CXCL11, Lymphotactin, M-CSF, MIG/CXCL9,

MIP-3a/CCL20, MIP-3b/CCL19, NAP-2/CXCL7

14) High Sensitivity Cytokine/Chemokine (Cat# HSCYTMAG-60SK)

IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, GM-CSF, IFNg, TNFa

Isotyping

Immunoglobulin Isotyping (Cat# HGAM-301K)

IgA, IgG1, IgG2, IgG3, IgG4, IgM

Available Machines:

1) Automatic Chemical Analyzer, Johnson & Johnson’s VT350

2) Heska’s Animal Blood Counter

3) Platelet Aggregation Profiler Model PAP-4

4) MAGPIX Luminex Instrumentation

Charges: UNC customer charges are as follows:

Individual Clinical Test - $6.25/test.

Group Tests:

General Health Test - $28.00

Kidney Test - $22.00

Liver Test - $18.00

Hematological Test - $10.00/sample.

We bill customers monthly.

How to Order and Receive Data:Users should bring samples in proper storage tubes and fill out a request form (available on the door of our lab). After analyzing the samples, we normally fax the data to you. However, if there are many pages of data, Users should pick up the data at our lab. They may also pick up leftover samples.