Gene Expression
Mission:
For studies of gene expression, a highly sensitive and quantitative
“Real Time” RT-PCR Sequence Detector ABI 7700 and an Automated
Nucleic Acid Workstation ABI 6700, which can purify total RNA and DNA from
animal tissues, blood, or cultured cells, are available to assist researchers
on a cost-per-test basis of service since May 2001.
1) Assistance in development of new RNA/DNA detection systems. Includes consulting time and training when needed. ($50/hr )
2)Quantitative RT-PCR ABI 7700 user fee.
Researchers can use the ABI 7700 machine with their
own samples prepared at their laboratory. We can help how to design experiment and
how to prepare samples. A typical experiment requires 3 hr
of machine time including data analysis. The user fee is $40
per experiment.
3)
Total RNA (DNA) preparation on ABI 6700.
Preparing RNA samples of high quality is an essential
part of the procedures for quantitative gene expression studies. We can purify total
RNA samples from animal tissues, blood or cultured cells with the ABI 6700
robot system for a charge of $5 per sample. You can bring frozen tissues or tissue stores
in RNA later solution. Also we can isolate genomic DNA from the
same tissue samples.
The ABI 6700 automated machine can perform three other high throughput
features, such as, cDNA synthesis, RNA sample dilution and preparation of
7700 assay plate.
You can use this system with $100 user fee per experiment (we can supply
disposal materials).
4)
Mouse genomic DNA genotyping with ABI 7700.
The ABI 7700 sequence detection
system can genotype mice created by insertion of a selection marker gene, such
as “neo”, during a gene targeting experiment.
Specifically, we can perform genotyping into wild
type (0 copy of neo), heterozygote (1 copy of neo), and homozygote (2 copy
of neo) from tail or toe genomic DNA ($5 per sample).
5)
Full service Gene Quantification.
Upon request, we can provide a full
service to analyze gene expression quantitatively. This includes all the steps necessary for
designing and synthesizing Taqman probes and primers, and preparing RNA samples. The charge would be
dependent on the size of project, but would likely be around $150-200 per gene plus cost of materials.
5)
Synthesis and purification of fluorescent oligonucleotide probes.
The most expensive part of repetitive experiments
costs is due to the price of the fluorescent oligonucleotide probes which are
essential for the real time RT-PCR system. Using our own DNA synthesizer and
purification system in the DNA synthesis core facility, we can successfully
prepare probes at lower cost than any other place. Our price would be $150 per probe at a high
concentration.
In addition, the common endogenous probes for human, mouse, etc.
ß-actin and GAPDH can be economically shared by alliquoting.
6) Freezer
system of the ABI supplies.
For real time RT-PCR experiments, we should use only
ABI company’s chemical products, which are expensive. By sharing these
materials, you can reduce the cost of experiments and shipping time.
For the service of gene expression, customers should contact
us by Phone call or E-mail to
make reservation.
To make RNA or DNA purification and mouse genotyping, you can bring
samples directly and fill the request forms.