Multiplexed Biomarker Immunoassays

We now offer multiplexed biomarker immunoassays for Cytokine/Chemokine detection, metabolism, toxicity, cancer biomarkers, and many other disease states by using the Luminex technology. The new Luminex MAGPIX system using magnetic bead-based multi-analytes provides a complete solution for rapid, accurate biomarker quantitation in a variety of sample matrices. This affordable system can perform up to 50 tests simultaneously in a single reaction volume, greatly reducing sample input (10-20ul/sample), reagents and labor while improving productivity. The MILLIPLEX magnetic bead-based multi-analyte panels from EMD Millipore Company (see below kits) enable researchers to gain more information faster without compromising reliability. Furthermore, an automated microplate washer from BioTek Company can enhance magnetic bead assays by complete plate biomagnetic separation during washing

Luminex MAGPIX system services and costs:

a) Full service covers whole procedures, ordering kits, performing experiments, analyzing data, and sending results (customers can only provide samples).

Cost; A Milliplex kit, 96 well formate for standard curves and controls (20 wells) and 76 unknown samples (~$200/analyte with current 25% discount, if 5 multiplexed it costs ~$1,000).

And plus research technician working hrs, ~8 hrs ($200).

b) If you do your own assays you must do the following:

Order your own reaction kits

Do your own experiments

$25 per machine use

$40 consultant and training (one time)

c) Available kits: (MAGPIX Millipex, June 2011)

For mouse samples:

Metabolism

1) Metabolic Hormone (Cat# MMHMAG-44K)

IL-6, Amylin (active), C-Peptide 2, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, Insulin,

Leptin, MCP-1, Pancreatic Polypeptide, PYY (total), Resistin, TNFa

2) Multi Species Steroid/Thyroid Hormone ( Cat# STTHMAG-21K)

Cortisol, Estradiol, Progesterone, T3, T4

3) IGF Binding Protein (Cat# MIGFBPMAG-43K)

IGFBP1, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IGFBP7

Inflammation/Immunology

4) Cytokine/Chemokine Panel I (Cat# MCYTOMAG-70K)

IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17a, Eotaxin,

G-CSF, GM-CSF, IFNg, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1a, MIP-1b, MIP-2, RANTES, TNFa, VEGF

5) Cytokine/Chemokine Panel II (Cat# MCYP2MAG)

IL-16, IL-21, IL-22, IL-25/IL-17, IL-28B, EPO, Exodus-2, Fractalkine, MCP-5, MIP-3a, MIP-3b, TARC

6) Cytokine/Chemokine Panel III (Cat# MCYP3MAG)

IL-20, IL-23, Il-27, IL-33, MDC, TIMP-1

For human samples:

Metabolism

1) Metabolic Hormone ( Cat# HMHMAG-34K)

IL-6, Amylin (active), C-Peptide 2, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, Insulin,

Leptin, MCP-1, Pancreatic Polypeptide, PYY (total), Resistin, TNFa

2) Liver Protein (Cat# HLPPMAG-57K)

AFP, ANGPTL3, ANGPTL4, ANGPTL6, FABP1, FGF-19, FGF-21, FGF-23, HGF

3) Multi Species Steroid/Thyroid Hormone ( Cat# STTHMAG-21K)

Cortisol, Estradiol, Progesterone, T3, T4

Neuroscience

4) Neurological Disorders Panel 2 (Cat# HND2MAG-39K)

AGP, Ceruloplasmin, Haptoglobin, SAP

Toxicity

5) Kidney Toxicity Panel 1 (Cat# HKTX1MAG-38K)

KIM-1, Osteopontin, Renin, TFF-3

6) Kidney Toxicity Panel 2 ( Cat# HKTX2MAG-38K)

B2-Microglobulin, Clusterin, Cystatin C

7) Kidney Toxicity Panel 3 (Cat# HKYX3MAG-38K)

KIM-1, Renin, TFF-3

8) Kidney Toxicity Panel 4 ( Cat# HKTX4MAG-38K)

Albumin, B2-Microglobulin, Clusterin, Cystatin C, Osteopontin

Cancer Biomarker

9) Circulating Cancer Biomarker Panel 1 (Cat# HCCBP1MAG-58K)

IL-6, IL-8, AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, FGF2/bFGF, HCGb, HE4, HGF, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, SDF-1a+b/CXCL12, sFas, sFasL, TGFa, TNFa, TRAIL,

VEGF

10) Circulating Cancer Biomarker Panel 2 (Cat# HND2MAG-39K)

Antithrombin III, Coagulation factor XIII, Complement factor H (CFH), Extracellular matrix protein 1 (ECM1), Vitamin D binding protein, Vitronectin

Inflammation/Immunology

11) Cytokine/Chemokine Panel I(Cat# MCYTOMAG-70K)

IL-1a, IL-1b, IL-1ra, IL-2, sIL-2Ra, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13,

IL-15, IL-17a, sCD40L, EGF, Eotaxin, FGF-2, Fit-3 ligand, Fractakine, G-CSF, GM-CSF, GRO, IFNa2, IFNg,

IP-10, MCP-1, MCP-3/CCL7, MDC/CCL22, MIP-1a, MIP-1b, PDGF-AA, PDGF-AB/BB, RANTES, TGFa, TNFa,

TNFb, VEGF

12) Cytokine/Chemokine Panel II

IL-16, IL-20, IL-21, Il-23, IL-28a, IL-33/NF-HEV (mature), 6CKine/CCL21, BCA-1/CXCL13, CTACK/CCL27, ENA-78/CXCL5, Eotaxin-2/CCL24/MPIF-2, Eotaxin-3/CCL26, I-309/CCL1, LIF, MCP-2, MCP-4,

MIP-1d (MIP-5/CCL15), SCF, SDF-1a+b/CXCL12, TARC/CCL17, TPO, TRAIL/TNFSF10, TSLP

13) Cytokine/Chemokine Panel III (Cat# HCYP3MAG-63K)

IL-11, IL-29/IFNg2, GCP-2/CXCL6/LIX, HCC-1/CCL14a, I-TAC/CXCL11, Lymphotactin, M-CSF, MIG/CXCL9,

MIP-3a/CCL20, MIP-3b/CCL19, NAP-2/CXCL7

14) High Sensitivity Cytokine/Chemokine (Cat# HSCYTMAG-60SK)

IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, GM-CSF, IFNg, TNFa

Isotyping

Immunoglobulin Isotyping (Cat# HGAM-301K)

IgA, IgG1, IgG2, IgG3, IgG4, IgM

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