Biochem 666

X-ray crystallography of macromolecules

Ke; 966-2244, hke@med.unc.edu; 1/08/14-2/10/14; 9:00-9:50, MWF; 3007 Genetics Medicine.

a. Crystallization of macromolecules

b. Crystal symmetry and space group

c. Nature of X-rays and principles of diffraction of a single crystal

d. Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement

e. Phase improvement

f. Preparation of heavy atom derivatives

g. Interpretation of electron density and model building of a protein structure

h. Refinement of a raw macromolecular structure