Nov 04, 2014
from 11:00 AM to 12:00 PM
|Contact Name||Amanda Chang|
Open to the public
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"Histone chaperones in transcription and DNA repair: the usual and unusual suspects"
Karolin Luger, PhD
HHMI Investigator and University Distinguished Professor
Department of Biochemistry and Molecular Biology
Colorado State University
The Luger lab studies the structure and function of large macromolecular assemblies involved in chromosome organization. They have years of experience in studying the structural biology of nucleosomes and chromatin, in complex with associated proteins. Their primary approach is x-ray crystallography. More recently, they have investigated the role of the posttranslational modifications of histones on nucleosome and chromatin structure and dynamics, and the structure and mechanism of histone chaperones in nucleosome assembly and disassembly.
Abstract: Nucleosomes are large macromolecular complexes that are assembled and disassembled in a stepwise manner. Nucleosome assembly and disassembly processes are essential during DNA replication, transcription, and repair, and are orchestrated by multiple protein complexes. Histone chaperones are multifunctional proteins that bind and shuttle histones into the nucleus, assemble and disassemble nucleosomes in an ATP-independent manner, and regulate transcription through largely unknown mechanisms. Several structurally unrelated types of histone chaperones, each with multiple family members, are known. Some histone chaperones operate equally well with all types of histones, whereas others are specific to types of histones, or even histone variants and function exclusively in replication-independent pathways. I will discuss various aspects of histone chaperone function and their regulation by post-translational modifications, and highlight different mode(s) of histone chaperone – histone interactions with possible roles in histone folding. Finally, I will introduce a chromatin architectural protein that has dual functions as a histone chaperone in gene transcription and DNA repair.
Hosted by Graduate Students:
Cassandra Hayne and Amy Howard