"Use of insertional mutagenesis to discover novel regulators of transcription, transformation and drug resistance"


When Apr 07, 2009
from 11:00 AM to 12:00 PM
Where 1131 Bioinformatics Auditorium
Contact Name
Contact Phone 919-843-9984
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Dr. Stark is a Distinguished Scientist of the Lerner Research Institute and Professor of Genetics, Case Western Reserve University.  His lab studies several different aspects of signaling pathways in mammalian cells, using most of the standard tools of molecular biology and also novel methods of generating mutant mammalian cells that are defective in signaling. The general objectives are to define mechanisms of signaling and its regulation and to identify hitherto unrecognized components of specific pathways. The major disease targets are cancer and inflammation, with which the great majority of our work is concerned. Currently, we are developing insertional mutagenesis with retroviral or lentiviral vectors as a major new technique, in close collaboration with Andrei Gudkov's laboratory. The vectors include a strong promoter which, when inserted in or near a gene, generates dominant negative products (proteins, partial proteins or antisense RNAs) which inactivate the expression or function of a protein required for signaling. Identification of the insertion site identifies the relevant gene and encoded protein. This methodology has very broad applications, including but not limited to the specific systems under investigation in our laboratory.

To learn more, please visit his website: www.lerner.ccf.org/molgen/stark/.


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