May 10, 2013
from 03:00 PM to 04:00 PM
|Where||G030 Bondurant Hall|
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Dr. Srinivas Ramachandran from Fred Hutchinson Cancer Research Center works with Professor Henikoff (HHMI Investigator).
Title: "Chromatin and Transcription: From A to H2A.Z"
Abstract: Transcription in eukaryotic cells occurs in the context of chromatin and nucleosomes have been known to be a barrier to transcription. However, the nature of RNA polymerase (pol2) transit past a nucleosomal barrier is still an important unanswered question. In vitro single molecule studies have several models for how pol2 deals with the nucleosome, but these studies use a minimal system for transcription that do not include all possible effects of in vivo conditions like histone post translational modifications, histone variants and the complete pol2 transcription machinery. Here, we developed an in vivo methodology to study pol2 transit through chromatin at base-pair resolution. In this talk, I will describe effect of chromatin on pol2 action in vivo and the effect of H2A.Z, a histone variant on pol2 kinetics in vivo.
Host: Nikolay Dokholyan