Associate Professor of Pharmacy, UNC-CH
PHD - California Institute of Technology
HONORS & AWARDS
The Singleton Laboratory is interested in understanding the molecular basis for the develoment and transmission of microbial drug resistance and the discovery and exploitation of new strategies for controlling drug-resistant microorganisms. We develop and adapt synthetic chemistry and synthetic biology methods to provide new molecular tools – both biologically active small molecules and innovative platforms – for hypothesis-driven biological research and pharmaceutical discovery. This important foundation of our program offers both chemically-oriented and biologically-oriented researchers new opportunities for the development of integrated, multi-disciplinary knowledge and technologies.
- chemical synthesis
- synthetic biology
- combintorial biochemistry
- molecular biophysics
- high-throughput screening
- synthetic nucleotide analogs, designed peptides, organometallic complexes
- DNA repair and recombination related to drug resistance
- genomic approaches for revealing antibacterial drug targets
- designed genetic selections for directed evolution of unnatural enzymes
- molecular mechanisms related to the evolution of drug resistance
- protease inhibitor resistance in human viruses
- new antimicrobial targets and strategies
- new paradigms for antimicrobial drug development
- new lead compounds via HTS
- new macromolecular therapeutics
- new enzymes
- Lee, A.M., Wigle, T.J., and Singleton, S.F.*, “Two-Tiered High-Throughput Screening-Compatible Assay for Inhibitors of RecA Activities,” Analytical Biochemistry (in press).
- Wigle, T.J., and Singleton, S.F.*, “Directed Molecular Screening for RecA ATPase Inhibitors,” Bioorganic & Medicinal Chemistry Letters, DOI 10.1016/j.bmcl.2007.04.013 (2007).
- Cline, D.J., Holt, S.L., and Singleton, S.F.*, “Inhibition of Escherichia coli RecA by a Rationally Redesigned Helical Peptide,” Organic & Biomolecular Chemistry, DOI DOI: 10.1039/B703159A (2007).
- Singleton, S.F.*, Roca, A.I., Lee, A.M., and Xiao, J., “Probing the Structures of RecA-DNA Filaments. Advantages of a Flurescent Guanine Analog,” Tetrahedron 63: 3553-3566 (2007).
- Lee, A.M., Xiao, J., and Singleton, S.F.*, “Origins of Sequence Selectivity in Homologous Genetic Recombination: Insights from Rapid Kinetic Probing of RecA-mediated DNA Strand Exchange,” Journal of Molecular Biology 360: 343-359 (2006).
- Xiao, J., Lee, A.M., and Singleton, S.F.*, “Direct evaluation of a kinetic model for RecA-mediated DNA strand exchange: Importance of nucleic acid dynamics and entropy during homologous genetic recombination,” ChemBioChem 7: 1265-1278 (2006).
- Lee, A.M. and Singleton, S.F.*, “Intersubunit Electrostatic Complementarity in the RecA Nucleoprotein Filament Regulates Nucleotide Substrate Specificity and Conformational Activation,” Biochemistry 45: 4514-4529 (2006).
- Wigle, T.J., Lee, A.M., and Singleton, S.F.*, “Conformationally Selective Binding of Nucleotide Analogs to Escherichia coli RecA: A Ligand-Based Analysis of the RecA ATP-Binding Site,” to Biochemistry 45: 4502-4513 (2006).
- Xiao, J., Lee, A.M., and Singleton, S.F.*, “Construction and Evaluation of a Kinetic Scheme for RecA-mediated DNA Strand Exchange,” Biopolymers 81: 473-496 (2006).
324 Beard Hall
Campus Box # 7568
3097 Genetic Medicine Bldg
Chapel Hill, NC 27599