Robert McGinty

Research: Mechanisms of epigenetic signaling

Robert McGinty

Assistant Professor of Pharmacy
Joint Appointment in Biochemistry and Biophysics
MD/PhD - Rockefeller University

125 Mason Farm Road, 3210 Marsico Hall
CB# 7363
Chapel Hill, NC 27599-7363
919-843-4912

McGinty Lab Website

HONORS & AWARDS

  • 2017 Searle Scholar Award
  • 2017 Pew-Stewart Scholar for Cancer Research

RESEARCH

Despite every cell in the human body having a nearly identical genetic sequence, divergent patterns of gene expression lead to the development of diverse cell types and functions. These patterns are established through epigenetic changes to the composition and structure of chromatin, the physiologic state of the genome. The repeating unit of chromatin is the nucleosome, in which segments of genomic DNA are wrapped around molecular spools of histone proteins. By displaying diverse combinations of histone post-translational modifications, the nucleosome serves as an active signaling hub in the regulation of genome-templated processes. These processes, including gene expression, DNA replication and DNA damage repair, play fundamental roles in cell development and maintenance of genomic integrity. As such, inappropriate regulation of the epigenetic enzymes that write, read and erase these modifications is correlated with many human diseases, especially cancer.

Over the past two decades, structural and biochemical studies using peptide fragments of histone proteins have elucidated many mechanisms through which epigenetic enzymes recognize histone sequences and modification patterns. However, these peptide-based approaches preclude a fundamental understanding of how epigenetic signals are integrated within more physiologic states of chromatin.

McGinty Laboratory studies mechanisms governing epigenetic signaling at the nucleosome and chromatin levels. We use protein chemistry to reconstitute ‘designer’ nucleosomes and chromatin, containing defined patterns of post-translational modifications. When paired with structural biology, including X-ray crystallography and single particle cryo-EM, this allows us to interrogate mechanisms governing specific epigenetic signaling pathways at atomic resolution. Together with the Center for Integrative Chemical Biology and Drug Discovery, my lab will leverage our structural studies toward the design of small molecules to disrupt chromatin signaling pathways for basic research and therapeutic goals.

REPRESENTATIVE PUBLICATIONS PubMed(click for Full Publication List)

  • McGinty RK, Tan S. Recognition of the nucleosome by chromatin factors andenzymes. Current opinion in structural biology. 2016; 37:54-61.
  • Girish TS, McGinty RK, Tan S. Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate. Journal of molecular biology. 2016; 428(8):1531-43.
  • McGinty RK, Tan S. Nucleosome structure and function. Chemical reviews. 2015; 115(6):2255-73.
  • McGinty RK, Henrici RC, Tan S. Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome. Nature. 2014; 514(7524):591-6.
  • Tang Z, Chen WY, Shimada M, Nguyen UT, Kim J, Sun XJ, Sengoku T, McGinty RK, Fernandez JP, Muir TW, Roeder RG. SET1 and p300 act synergistically, throughcoupled histone modifications, in transcriptional activation by p53. Cell. 2013; 154(2):297-310.
  • Kim J, Kim JA, McGinty RK, Nguyen UT, Muir TW, Allis CD, Roeder RG. The n-SET domain of Set1 regulates H2B ubiquitylation-dependent H3K4 methylation. Molecular cell. 2013; 49(6):1121-33.
  • Whitcomb SJ, Fierz B, McGinty RK, Holt M, Ito T, Muir TW, Allis CD. Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2. The Journal of biological chemistry. 2012; 287(28):23718-25.
  • Ruthenburg AJ, Li H, Milne TA, Dewell S, McGinty RK, Yuen M, Ueberheide B, Dou Y, Muir TW, Patel DJ, Allis CD. Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactions. Cell. 2011; 145(5):692-706.
  • Scheuermann JC, de Ayala Alonso AG, Oktaba K, Ly-Hartig N, McGinty RK, Fraterman S, Wilm M, Muir TW, Müller J. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Nature. 2010; 465(7295):243-7.
  • Fierz B, Chatterjee C, McGinty RK, Bar-Dagan M, Raleigh DP, Muir TW. Histone H2B ubiquitylation disrupts local and higher-order chromatin compaction. Nature chemical biology. 2011; 7(2):113-9.
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