Biochem 667

Macromolecular Crystallographic Methods

Collins (Course Director; 966-6869; collins1@med.unc.edu), Redinbo, Miley;  TTh 2/12/14-4/25/14 (Offered in Even Years); 10:30-12:00; 3007 GM; Note 1 credit Prerequisite BIOC 666 or permission of course director.  This is a practical workshop for serious students of protein crystallography.  There will be a laboratory component that will be arranged to accommodate the schedules of students and faculty.  In the laboratories, students will crystallize protein, collect and process their data, determine phases and refine their structures.  There is no specific text required, but Jan Drenth “Principles of Protein X-ray Crystallography” is recommended.

Part I:

  • Data Collection (Collins)
  • Bragg’s law and Ewald’s construction for visualizing diffraction; Detectors; Symmetry
  • Corrections; Integration; Data analysis

Part II:

  • Phase determination (Redinbo)
  • MR; MIR; AS; MAD; Direct methods

Part III:

  • Refinement (Collins

a. Computational

i. Targets

ii. Techniques

b. Manual intervention

Laboratory portion:
Crystallize lysozyme; Collect data set (teams); Data reduction; Molecular replacement; Introduction to “O”