Commonly Used Fluorophores

ProbeEx (nm)Em (nm)MWNotes
Wild Type GFP 396,475 508 26k

EGFP

488 507 26k weak dimer, (Clontech)
Fluorescein 495 519 389 FITC; pH sensitive
Cy3 (512);550   
570;(615)  
767 QY 0.15
Propidium Iodide (PI) 536 617 668.4   
TRITC 547 572 444 TRITC
tdTomato 554 581 tandem dimer
DsRed2 ("RFP") 563 582 ~110k (Clontech)
Lissamine Rhodamine B   
570 590
TurboFP602 574 602 26k dimer, (Evrogen)
mCherry 587 610 monomer
TurboFP635 588 635 26k dimer, (Evrogen)
mKate (TagFP635) 588 635 monomer (Evrogen)
Katusha 588 635 dimer
Texas Red 589 615 625 Sulfonyl chloride
Cy5 (625);650 670 792 QY 0.28
Cy7 743 767 818 QY 0.28