Optical Systems


IVIS® Optical System


The IVIS Kinetic optical imaging system is a real time imaging system enabling acquisition of biologically relevant events within milliseconds. This IVIS systems can be used for in-vivo, ex-vivo, and in-vitro imaging studies and are ideal for both high SNR quantitative scans and high throughput screening applications. The IVIS Lumina optical imaging system is similar to the Kinetic but does not have real time capabilities.  The Lumina is located in the Genetic Medicine Building room UB61.  The purpose of this machine is to facilitate optical imaging studies with animals located in the GMB vivarium that cannot be returned from outside laboratories.  

Study examples

  • Oncology Research
  • Infectious Disease
  • Inflammation
  • Metabolic Disease
  • Neurology
  • Gene Therapy
  • Stem cell Biology
  • Cardiovascular Disease
  • Drug metablism
  • Cherenkov radiation imaging

Main advantages of this system include:

  • Highly sensitive EMCCD camera for in vivo and in vitro imaging of fluorescence and bioluminescence
  • Rapid image readout speeds with frame transfer technology
  • Light-tight injection port, supporting a syringe injector system for real time compound and/or substrate administration
  • Adjustable field of view 5 cm2 -12.5 cm2
  • Highly sensitive EMCCD camera
  • CCD camera is thermoelectrically cooled to -80 C for low dark current and low noise
  • 10 position excitation filters
  • 25 emission filters ranging from 500nm-800nm
  • Fast frame rate for real time imaging

Click here to view IVIS Kinetic Calendar

Click here to view IVIS Lumina Calendar

Sample Images (click to enlarge):

Kinetic_Samples1_Icon.jpg  Kinetic_Samples2_Icon.jpg

FMT-2500 in vivo optical imaging system


The FMT 2500 system (VisEn, Inc.) is a 3D tomography in vivo optical imaging system, which can provide much more accurate quantification and spatial distribution of fluorescent probes in live animals or specimen. The system combined with NIR fluorescence probes will enable scientists understand biological targets, processes and pathways in physiologically-relevant environments non-invasively, and monitor disease progression and treatment efficacy longitudinally.

Study examples

  • 3-D localization of markers for: hemophilia, arthritis, tumor cells, inflammation.  

Main advantages of this system include:

  • Ability to tomographically image and quantify fluorescence probes throughout the entire depth of mouse models with high sensitivity and accuracy to pico-molar concentration
  • Possible co-registration with microCT, PET, SPECT or MRI.
  • Measure and monitor multiple biological processes simultaneously with 3D image generation.
  • Imaging field of view: 3x6 cm; resolution: 2 mm
  • 3D tomographic imaging
  • Four laser channels for various NIR probes:
  • 635 nm Channel (Excitation), Emission range = 650 - 670 nm
  • 670 nm Channel (Excitation), Emission range = 690 - 740 nm
  • 745 nm Channel (Excitation), Emission range = 770 - 800 nm
  • 790 nm Channel (Excitation), Emission range = 805+ nm

Some fluorescent probes can be obtained from the following link: http://www.perkinelmer.com/invivoimaging

Click here to view this calendar.

Sample Images:

 FMT_2500_Samples1_Icon.jpg  FMT_2500_Samples2_Icon.jpg

**Click here to find out wavelength information for some common fluorophores.**