Research Assistant Professor
- B.S., Universidad Nacional de Mar del Plata, Argentina, 1995
- PhD, University of Alabama at Birmingham, 2002
- Postdoc, UNC Chapel Hill
Lisa Sharek, Research Technician
Alejandra Valdivia, PhD, Postdoctoral Fellow
The GTPases of the Rho family control almost every aspect of cell behavior, including cell migration, cell-cell adhesion, cell polarity, contractility, invasion, proliferation and differentiation, and gene transcription. Rho GTPases function as a link between surface receptors and the actin cytoskeleton. Activation of Rho proteins is mediated by guanine-nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. The signal is terminated by hydrolysis of GTP to GDP a reaction that is stimulated by GTPase activating proteins (GAPs). Humans have more than 70 Rho-GEFs and approximately 60 Rho-GAPs, allowing the cell to regulate the activity of 22 Rho proteins through multiple pathways.
My lab focuses on the mechanisms that control the targeting and regulated activation of Rho-GTPases. These processes have been shown to play essential roles both in normal cell function, as well as in abnormal cell behavior, including metastasis, atherosclerosis, inflammation and hypertension.
- V. Krishna Subbaiah, P. Massimi, S. Shi Boon, M.P. Myers, L. Sharek, R. Garcia-Mata and L. Banks. 2012. The Invasive Capacity of HPV Transformed Cells Requires the hDlg-dependent enhancement of SGEF/RhoG Activity. PLoS Pathogens (in press).
- E. Boulter and R. Garcia-Mata. 2012. Role of RhoGDI1 in the regulation of RhoGTPases. I. Methods in Molecular Biology, 827:97-105.
- Boulter, E., Estrach, S., Garcia-Mata, R., and Feral, C., 2011. Off the beaten paths: alternative and crosstalk regulation of Rho GTPases. FASEB J. (Oct 28. Epub ahead of print).
- Roney KE, O'Connor BP, Wen H, Holl EK, Guthrie EH, Davis BK, Jones SW, Jha S, Sharek L, Garcia-Mata R, Bear JE, Ting JP. 2011. Plexin-b2 negatively regulates macrophage motility, rac, and cdc42 activation. PLoS One.;6(9): e24795.
- C. Guilluy, R. Garcia-Mata and K. Burridge. 2011. Rho protein crosstalk: another social network? Trends in Cell Biology 21, 718-726.
- C. Guilluy , A.D. Dubash, and R. Garcia-Mata. 2011. Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols 6, 2050-2060.
- R. Garcia-Mata, E. Boulter and K. Burridge. 2011. The Invisible Hand: Regulation of Rho GTPases by RhoGDIs. Nature Reviews in Molecular and Cell Biology.12:493-504 (featured on the cover).
- C. Guilluy , V. Swaminathan , R. Garcia-Mata, E. O'Brien III, R. Superfine, and K. Burridge. 2011. The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. Nat Cell Biol 13:722-727. .
- A.D. Dubash, M. Srougy, C. Guilluy, E. Boulter , K. Burridge and R. Garcia-Mata. 2011. The Small GTPase RhoA Localizes to the Nucleus and is Activated by Net1 and DNA Damage Signals. PLoS one 6, e17380.
- E. Boulter*, R. Garcia-Mata*, C. Guilluy, A.D. Dubash, G. Rossi, P.J. Brennwald and K. Burridge.2010. Regulation of RhoGTPase crosstalk, degradation and activity by RhoGDI1. Nature Cell Biology, 12, 477-483 (*equal contribution).
- S. Goicoechea, H. Prentice-Dunn, B. Bednarski, R. Garcia-Mata, X. Ding, H.J. Kim, and C.A. Otey. 2009. Palladin Expression Contributes to Invasive Motility in Human Breast Cancer Cells. Oncogene. 2009;28: 587-98
- R. Garcia-Mata, A.D. Dubash, L. Sharek, H.S. Carr, J.A. Frost and K. Burridge. 2007. The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity. Mol. Cell. Biol, 27: 8683-8697.
- A.D. Dubash, K. Wennerberg, R. García-Mata, M.M. Menold, W.T. Arthur, K. Burridge. 2007. A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin. J. Cell Sci. 120: 3989-3998.
- J. Van Buul, M. Allingham, T. Samson, J. Meller, E. Boulter, R. Garcia-Mata and K. Burridge. 2007. RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. J. Cell Biol. 178: 1279-1293.
- R. Garcia-Mata and K. Burridge. 2007. Catching a GEF by its tail. Trends in Cell Biology. 17, 36-43.
- S. Goicoechea, D. Arneman, A. Disanza, R. Garcia-Mata, G. Scita and C. A. Otey. 2006. Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells. J Cell Sci. 119:3316-24
- R. Garcia-Mata, K. Wennerberg, W. T. Arthur, N. K. Noren, S. M. Ellerbroek and K. Burridge. 2006. Analysis of activated GAPs and GEFs in cell lysates. Methods Enzymol. 406:425-37
- T. Szul, R. Garcia-Mata, E. Brandon, S. Shestopal, C. Alvarez and E. Sztul. 2005. Dissection of membrane dynamics of the ARF-guanine nucleotide exchange factor GBF1. Traffic. 6:374-85
- R. Garcia-Mata and E. Sztul. 2003. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. 4:320-5
- R. Garcia-Mata, T. Szul, C. Alvarez and E. Sztul. 2003. ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1. Mol Biol Cell. 14:2250-61
- C. Alvarez, R. Garcia-Mata, E. Brandon and E. Sztul. 2003. COPI recruitment is modulated by a Rab1b-dependent mechanism. Mol Biol Cell. 14:2116-27
- R. Garcia-Mata, Y. S. Gao and E. Sztul. 2002. Hassles with taking out the garbage: aggravating aggresomes. Traffic. 3:388-96
- C. Alvarez, R. Garcia-Mata, H. P. Hauri and E. Sztul. 2001. The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic. J Biol Chem. 276:2693-700
- R. Garcia-Mata, Z. Bebok, E. J. Sorscher and E. S. Sztul. 1999. Characterization and dynamics of aggresome formation by a cytosolic GFP-chimera. J Cell Biol. 146:1239-54
- D. S. Nelson, C. Alvarez, Y. S. Gao, R. Garcia-Mata, E. Fialkowski and E. Sztul. 1998. The membrane transport factor TAP/p115 cycles between the Golgi and earlier secretory compartments and contains distinct domains required for its localization and function. J Cell Biol. 143:319-31