Histology - Sample Images

Basic Histological Stains

 

DAB and Fluorescent IHC

 

 

 

histo6

CME stain (mouse artery)
Elastic Fibers—black,
Collagen, mucin—green
Muscle—red/brown

 

histo8

Masson Trichrome stain (Mouse heart valves)
Nuclei—black
Cytoplasm, muscle fibers—red
Collagen, mucin—blue

histo1

Periodic Acid-Schiff stain (PAS)
Glycogen—bright rose/red
Nuclei—blue

histo7
Picrosirius Red stain (gut)
Collagen—red

 

 

histo2

Prussian Blue stain for iron
Nuclei, hemofuschin—bright red
Hemosiderin(iron)—blue
Background—pink

 

 

 

histo4
Sudan IV stain- lipids
Fat—red
Nuclei—blue

 

histo3

Von Kossa stain- Calcium deposits
Calcium—black
Nuclei, muscle—red

 

histo9

BrdU staining

 

histo5
Biocytin IHC with DAB visualization- neuron

 

 

histo10

Lysozyme IHC with DAB visualization

 

 

histo11

Fluorescent double-label with GFP and Biocytin-SATR (neuron)

Green- GFP

Red- Biocytin-SATR

Yellow- Co-expression