The Zeiss 510 Meta is housed at 230A Taylor Hall.
The Zeiss LSM 510 META NLO confocal, multi-photon system can be used with either a Zeiss Axiovert 200M inverted or Zeiss Axioplan 2 upright microscope. The system is equipped with 4 laser light sources including an argon laser (458, 488, or 514 nm), two HeNe lasers (543 and 647 nm), and a Coherent Chameleon femtosecond pulsed Ti:Sapphire laser for multi-photon microscopy, which is tunable between 690-1040nm. Some of the system features:
- Spectral analysis - The META detector allows for spectral analysis of your samples, spectral unmixing of different dyes and auto-fluorescence is available.
- Co-localization of proteins - The LSM 510 META can perform simultaneous multi-color imaging, allowing users to detect the distribution of various fluorescently-tagged proteins. It is also possible to perform live cell imaging using (GFP) green, cyan (CFP) - and yellow (YFP) fluorescent proteins.
- Fluorescent Resonant Energy Transfer (FRET) - The availability of excitation and detection channels for CFP-YFP means that users can perform FRET experiment to study dynamic protein-protein interaction in live cells.
- Photo-bleaching (FRAP and FLIP) - Zeiss LSM 510 META provides user-defined, tunable photo-bleaching capability. This allows users to perform experiments such as fluorescence recovery after photo-bleaching (FRAP) and fluorescence loss in photo-bleaching. These fluorescence techniques are extremely powerful in study protein trafficking in and out of various sub cellular compartments.
- Fluorescence Intensity Measurement - Users can measure fluorescent intensity using LSM 510 META at any given region of interest. It is important to note that comparing fluorescent intensity from one image to another requires internal normalization standards. Please consult the facility staff for suggestions.
- Photoactivation and Photoconversion - Taking advantage of the capability of the scanning laser to specifically excite (or photobleach) a user-defined region of interest, users can also specifically photo-activate or photo-convert a subpopulation of molecules in living cells in order to follow the dynamics of the targeted population of molecules.
- Multi-photon Microscopy - The Facility has just acquired a Coherent Chameleon femtasecond pulsed Ti;sapphire laser for multi-photon microscopy that makes multi-photon as easy as confocal. The laser is user friendly and tunable between 690-1040nm. The system also has an external non-descanned detector for multi-photon imaging. The advantages of multi-photon microscopy are 1) the ability to image deeper into tissue than confocal = 100µm 2) excitation only occurs in the focal plane, no out of focus photobleaching 3) ability to photo-uncage caged compounds in a small volume and 4) lower phototoxicity due to imaging with longer wavelengths.
- Z-sectioning - It is the unique feature of a confocal microscope to perform Z-section on biospecimens. This is different from image deconvolution. While deconvolution relies on mathematical algorithms to rid the image of out of focus intensity data, confocal microscope relies on a small pinhole to block out of focus light.