Ion/Liquid Transport

The CF Center also has extensive capabilities to perform classical Ussing chamber studies on large numbers of tissues for screening purposes. Extensive experience has accrued in developing the appropriate quality control procedures to assure accurate and reproducible testing of compound activities in this assay.

The CF Center also has extensive experience in characterizing airway surface liquid physiology, in response to candidate therapeutic agents, under the “thin film” ASL technique (Figure 6). In “standard” versions of this technique, a combination of airway surface liquid (ASL) volume as measured by confocal techniques, indices of ion transport processes mediating ASL volume homeostasis from transepithelial microelectrode voltage measurements, coupled with micropipette liquid sampling for measurements of drug concentrations, can be made. Note, drugs can be added to the basolateral compartment of these preparations, the luminal compartment in perfluorocarbon, or indeed, more recently, by an interface to ultrasonic nebulizers that deliver aerosols to cultured airway surfaces at rates that mimic aerosol deposition in vivo (e.g., 20 nl/cm2/min – see Figure 7).

 


Inquiries

Questions? If you are interested in learning more about how the UNC CF Center can help further your research, or if you are interested in collaborating with us, please contact our CF Center Director, Dr. Richard Boucher:

7011 Thurston-Bowles Bldg.
The University of North Carolina at Chapel Hill
Campus Box #7248
Phone: (919) 966-1077
Fax: (919) 966-5178
Email: richard_boucher@med.unc.edu

[top]