Immunotechnologies (ELISA & Multiplex)

The AA Core's immunotechnologies (IT) component provides consultation, ordering, and offering assay service and support for most commercially available kits in both traditional ELISA/EIA and bead-based multiplex formats. The AA Core typically receives discounts of 20% or more from preferred vendors and the Core’s innovative cost-sharing system allows users to purchase ELISA reagents they need in more cost-effective quantities and receive credits toward future assays when other users have need of them. When combined with the already lower cost of bulk reagents – the most requested of which are kept in-stock and can be run for as little as $100/plate – paying to have your ELISA work done by the AA Core can save a user hundreds or even thousands of dollars a year.

That being said, while reliable and relatively inexpensive, ELISAs do typically require a sizable volume of sample for each target being assayed. This can be a major disadvantage when it comes to samples and sample types that are rare or difficult to acquire. Luminex's bead-based xMAP technology, on the other hand, allows for simultaneous assay of up to 100 different analytes using as little as 10 uL per sample. The AA Core's Bio-Plex 200 system utilizes this technology, allowing investigators to quickly assess the significance of multiple targets and acquire large amounts of data from very little sample. Bead-based assays also benefit from a very broad standard curve (frequently 5-10x that of ELISA), greatly reducing the need for repeat assays. For investigators whose samples are especially precious, the value of this technology’s ability to generate large amounts of publication-quality data from so little sample is estimable.

For more information about the immunoassay products and services the AA Core offers, please contact the Core Manager. To place a core use request, click here.

Instruments:

Services:

Current Services

Consultation: All prospective Core users receive up to one hour of complimentary consultation, including any assay research or purchasing advice, at no charge.

Assay Services: Ordering and immunoassay services are available through the AA Core at very reasonable rates. Just schedule an assay, drop off your samples, and in a week or less you’ll have data.

Plate Reading & Data Analysis: Your assay plate will be read and you will be sent an electronic copy of your results, including some basic statistics (mean, standard deviation, % CV) and information about the standard curve (graph, formula, curve fitting results). Users who prefer to prep and run their own assays may purchase this as a stand-alone service.

Sample Pickup & Delivery: Sample pickup and delivery service is available upon request. Please note that off-campus users pay a higher rate for this service due to the additional time and distance involved.

Pricing:

Service Price

Consultation/Assay Research (after first hour)

$25.00 per additional hour

Assay Materials

Varies by assay & vendor

Assay Service

$1.00 per well

Additional Processing/Handling

$1.00 per sample

Absorbance/Fluorescence/Luminescence Reading

$10.00 per plate

Multiplex Suspension Array Reading

$30.00 per plate

On-Campus Pickup/Delivery

$10.00 per trip

Off-Campus Pickup/Delivery

$20.00 per trip

To place a core use request, click here.

Submitting Samples:

To begin planning an immunoassay project with the AA Core, please contact the Core Manager to arrange a consultation. Once project details have been coordinated, samples may be submitted. All samples should be dropped off in Room 030 of the Glaxo Building, and new users are advised to make an appointment to ensure that someone will be available to receive their samples.

All samples should be submitted in ready-to-assay form on a minimum-binding 96-well plate (we recommend Fisher part number 12-565-505).  Tubed samples are also acceptable, but will be charged $1 per sample for additional processing and handling. Any pre-extraction or sample preparation procedures are the sole responsibility of the user, unless alternate arrangements have been made in advance with the Core Manager. Samples should be submitted in a 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order. For those who don't have them, prep plates are available upon request from the Core Manager.

Unless singlets are specifically requested , please provide sufficient volume for all samples to be assayed in duplicate, plus an additional 25uL. Samples to be diluted may instead be submitted at the volume required to dilute them up to 250-300uL evenly (e.g., 25uL for a 1:10 dilution).  All samples should be dropped off in Room 030 of the Glaxo Building, and prospective users are advised to make an appointment to ensure that someone will be available to receive their samples and the accompanying template.