Introduction to the Lentivirus-shRNA Facility

This UNC - Chapel Hill shared research core facility provides lentivirus based reagents for the inactivation of mammalian gene expression.

When Apr 10, 2014
from 11:15 AM to 12:15 PM
Where NC State University, College of Veterinary Medicine Research Bldg. Room 101
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Lentivirus

carrying shRNA or cDNA cargoes are available and are based on the

TRC1 and the ORFeome V8.1 libraries, respectively. The libraries are cataloged as single lentiviral-vector shRNA and cDNA clones, and target ~16,000 human and mouse genes. Each gene is targeted by 4-5 different shRNAs driven by the U6 promoter. cDNAs are expressed under the control of a CMV promoter. An overview of the technologies and reagents available within the core facility will be presented.

Seminar sponsored by NCSU