OTT Educational Series 2011-12

Events Listing of the OTT Educational Series

2011 - 2012 Events:

9-14-11: Mass Spectrometry Based Tissue Imaging (MALDI)

Presented by the Systems-Proteomics Core Facility, MALDI-Imaging Mass-spectrometry (IMS) is a novel technique at the fore-front of imaging-based scientific research.
This technique allows the acquisition of “Molecular” images for any tissue based on the distribution of proteins, lipids or small molecules. With extensive application to basic and clinical research, IMS is becoming a powerful tool for diagnosis and for biomarker discovery. This conference will reunite world-leaders in IMS who will present their personal experiences on this field, including applications to basic and clinical sciences, both for diagnoses and biomarker discovery. 


9-27-11: Next Generation Postlight Sequencing with Semiconductor Chips.

Join Dr. Jeremy Stuart of Life Technologies, to discuss next-generation PostLight™ sequencing with semiconductor chips. Ion Torrent has developed the world’s first semiconductor-based DNA sequencing technology, directly translating chemical information into digital data. DNA sequencing is performed with all natural nucleotides on Ion’s proprietary semiconductor chips, leveraging a billion years of evolution and a trillion dollars of investment to allow unprecedented scalability, speed, and costs according to Moore’s Law. Because Ion Torrent technology doesn't use light, it vastly reduces the cost and time of sequencing while still delivering next-generation sequencing throughputs. This may democratize the field of next-generation sequencing and have important implications for clinical, diagnostic, and research applications in which speed is of paramount significance. 


10-6-11: Automated high-Throughput qPCR Solutions

Sponsored by the Vironomics Core. The LightCycler 480 instrument is designed for the High Throughput analysis for gene expression studies and also for a variety of genotyping applications. To move this technology even further the instrument can be used for protein melting. This talk will cover the major applications that can be performed on an high throughput basis and also high light the RealTime Ready focus/custom panels – bringing chemistry (Universal Probes) of the reactions to the high throughput platform providing a seamless transition. All these accompanied by the separating unique qualities of the instrument make the high throughput a reality in the current molecular biology research scenario.
Dr. Rama Badugu is a field applications specialist for Roche Applied Sciences. Rama earned his Ph.D in Life Sciences from University of Hyderabad in India, with a focus on protein/enzymatic biochemistry and separation technologies. In his extensive post-doctoral training at University of Kentucky, Rama worked on Heterochromatin Biology in Drosophila Melanogaster, using classical genetics and modern molecular biology research tools, before moving to Roche.



10-7-11: Single-Molecule, Real-Time (SMRTtm)

 Monitoring of Biomolecules: DNA Sequencing and Beyond
Presented by Jonas Korlach, Ph.D., Co-founder and Scientific Fellow, Next Generation Applications and Technologies. 
Understanding the dynamics of biological processes is fundamental to understanding life itself. At Pacific Biosciences, we are developing applications to observe individual biomolecules at work in real time. The first is monitoring DNA synthesis by single DNA polymerase molecules, allowing the speed, processivity, and efficiency of the enzyme to be exploited for new capabilities in DNA sequencing. The power of this new sequencing technology - characterized by long readlengths and fast run times - is highlighted through examples from diverse applications, such as finishing genomes, characterizing transcript and gene fusion diversity, rapid pathogen sequencing, and the direct detection of epigenetic markers.  The same instrumentation can be used to eavesdrop on other fundamental life processes, including transcription, reverse transcription, splicing, translation, and multi-component biomolecular interactions. Several examples of this paradigm will be described, including the dynamics of translation initiation and elongation by single ribosomes and the dynamic action of anti-retroviral drugs on HIV reverse transcriptase.


10-13-11: Impact of Pharmacometris on Boceprevir and Telaprevir Approvals and HCV Clinincal Care

Dr. Pravin Jadhav, Pharmacometrics Team Leader at the U.S. Food and Drug Administration, and Dr. Michael Fried, Director of the UNC Liver Center in a discussion of the approaches used by the FDA to grant approval and the clinical impact of these drugs.  Sponsored by the Pharmacometrics Core Facility. 


1-27-12: Mouse Models I: Resources for Mouse Model Development at UNC.

 This symposium will feature speakers from core facilities and resource centers at UNC that focus on mouse model development.  The Animal Models Core provides a full range of genetic modification services for production of transgenic and knockout mice, including model design, transgene cloning and BAC recombineering, pronuclear microinjection, targeting vector construction, ES cell gene targeting, and blastocyst injection of targeted ES cells.  The Mutant Mouse Regional Resource Center functions as a repository and informatics database for mutant strains of mice; the center at UNC is one of four collaborating centers sponsored by the National Center for Research Resources (NCRR) at NIH.  The Collaborative Cross is a panel of inbred mouse lines developed as an optimal model for human genetic diversity.  The Gnotobiotic Core of the Center for Gastrointestinal Biology and Disease is available for projects requiring germ-free inbred and outbred rodent strains.

Dale O. Cowley          Animal Models Core for Transgenic and Knockout Mice
Jackie Brooks             The Mutant Mouse Regional Resource Center (MMRRC)
Darla Miller                 Collaborative Cross
Maureen A. Bower     National Gnotobiotic Rodent Resource Center (NGRRC) 


2-22-12: Mouse Models II: Resources for Phenotyping Mouse Models at UNC

  This symposium will feature speakers from four core facilities at UNC that focus on mouse model phenotyping.  The Mouse Cardiovascular Models Core includes an experimental surgical laboratory for high-throughput physiological phenotyping.  The Animal Clinical Chemistry and Gene Expression Laboratories provide blood chemistry tests, urinalysis, hematology, and other assays similar to routine clinical screens in humans.  The Animal Metabolism and Phenotyping Core of the UNC Nutrition Obesity Research Center allows the analysis of body composition with MRI technology, long-term evaluation of voluntary exercise, and other indices of energy expenditure and balance.  The Mouse Behavioral Phenotyping Laboratory, established by the Carolina Institute for Developmental Disabilities, provides assays for motor and sensory ability, learning and memory, and other domains of function.

Mauricio Rojas   Mouse Cardiovascular Models Core  
Hyung Kim         Animal Clinical Lab, Gene Expression & Mouse Genotyping Core
Daniel Pomp      Animal Metabolism & Phenotyping Core, UNC Nutrition Obesity Research Ctr
Sheryl Moy         Mouse Behavioral Phenotyping Lab, Carolina Institute for   Developmental Disabilities



 4-2-12: Animal Study Support and Imaging Resources at UNC

The small animal imaging core provides advanced in vivo imaging on animals, including MRI, PET, SPECT, CT, ultrasound, and optical imaging, with the state-of-the-art instrumentation and experienced staff.  The talk will be focusing on applications of these imaging techniques for a wide range of biomedical research including cancer, cardiovascular, neuroscience, and other research studies that can be benefit from imaging tools.
The Animal Studies Core makes available a central facility staffed by skilled technicians to promote reproducible experimentation in laboratory animals for UNC Investigators. This presentation will detail the services we provide to the research community.

Charlene Santos, Animal Studies Core
Hong Yuan, Biomedical Research Imaging Center (BRIC)


5-10-2012: Protein Production and Analysis Resources at UNC

Whether your research would be accelerated by the production of a protein or you need help analyzing the protein composition of a complex mixture, UNC Core Facilities can help you quickly and economically meet your needs. This workshop will provide information on the UNC Cores that support peptide and protein production and all aspects of protein analysis and the resources they can bring to bear on how to meet your research needs.

Center for Structural Biology - Mischa Machius
High Throughput Peptide Synthesis and Array Facility - Brain Strahl
Macromolecular Interactions Facility - Ashutosh Tripathy
Systems-Proteomics Center - Oscar Alzate
UNC Micheal Hooker Proteomics Center - David Smalley


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