Briefly, a cold adhesive tape is applied to the frozen sample, supporting the section as it is cut. The tape, with attached tissue, is rolled onto a cold adhesive-coated slide which is then cured with a flash of ultraviolet light. As a result, the adhesive coating is converted into a solvent-resistant plastic. The tape is then pulled away leaving the section tightly bonded to the slide.
The Tape-Transfer System preserves sample morphology and produces frozen sections with the same quality as those cut from paraffin blocks. Sections are wrinkle-free, uncompressed and fully intact. This system is ideal for projects requiring perfect morphology and/or ultra thin sections that reveal morphological detail and clarify structural relationships. CryoJane applications include histological stains, immunohistochemistry, immunofluorescence, in situ hybridization, laser capture microdissection, frozen tissue arrays and other molecular diagnostic studies.
The Histology Research Core Facility offers a variety of services, insuring that histological techniques are always available. Currently we provide histological and immunohistochemical services to users from UNC campuses and other research institutions. The Facility is located in Rooms 004 and 007 of the Glaxo Research Building. For further information please contact Kirk McNaughton, Histology Research Core Facility Director, at email@example.com (919) 966-1202 and check out our web page www.med.unc.edu/physiolo/research/histology-facility/