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Center for Structural Biology (CSB)



  1. Jones JC, Duffy JW, Machius M, Temple BR, Dohlman HG, Jones AM. The Crystal Structure of a Self-Activating G Protein {alpha} Subunit Reveals Its Distinct Mechanism of Signal Initiation. Science Signal. 2011 Feb 8;4(159):ra8. PMID: 21304159 [PubMed - in process]
  2. Bosch DE, Kimple AJ, Sammond DW, Muller RE, Miley MJ, Machius M, Kuhlman B, Willard FS, Siderovski DP. Structural Determinants of Affinity Enhancement between GoLoco Motifs and G-Protein {alpha} Subunit Mutants. J Biol Chem. 2011 Feb 4;286(5):3351-8. Epub 2010 Nov 29. PMID: 21115486 [PubMed - in process]

  3. 2010

  4. Sharaf NG, Barnes CO, Charlton LM, Young, GB, Pielak, GJ. A bioreactor for in-cell protein NMR. Journal of Magnetic Resonance. 2010; 202:140-146.
  5. Wu H, Turner C, Gardner J, Temple B, Brennwald P., The Exo70 Subunit of the Exocyst is an Effector for Both Cdc42 and Rho3 Function in Polarized Exocytosis, Mol Biol Cell. 2010 [Epub ahead of print]
  6. Kesner BA, Ding F, Temple BR, Dokholyan NV. N-terminal strands of filamin Ig domains act as a conformational switch under biological forces. Proteins 2010; 78(1):12-24.
  7. Kesner BA, Milgram SL, Temple BRS, Dokholyan NV. Isoform divergence of the Filamin family of proteins. Mol Biol Evol. 2010; 27(2):283-95


  8. Mudgil Y, Uhrig JF, Zhou J, Temple B, Jiang K, Jones AM.. Arabidopsis N-MYC DOWN-REGULATED-LIKE1, a Positive Regulator of Auxin Transport in a G-Protein-mediated Pathway. Plant Cell. 2009 Nov;21(11):3591-609.
  9. Friedman EJ, Temple BRS, Hicks SN, Sondek J, Jones CD, Jones AM, Prediction of protein-protein interfaces on G-protein b subunits reveals a novel phospholipase C b2 binding domain, J Mol Biol. 2009; 392(4):1044-54.
  10. Heenan EJ, Vanhooke JL, Temple BR, Betts L, Sondek JE, Dohlman HG. Structure and function of Vps15 in the endosomal G protein signaling pathway. Biochemistry 2009; 48:6390-401.
  11. Chen Z, de Serrano V, Betts L, Franzen S. Distal histidine conformational flexibility in dehaloperoxidase from Amphitrite ornata. Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):34-40.
  12. Kimple AJ, Soundararajan M, Hutsell SQ, Roos AK, Urban DJ, Setola V, Temple BR, Roth BL, Knapp S, Willard FS, Siderovski DP. Structural determinants of G-protein alpha subunit selectivity by regulator of G-protein signaling 2 (RGS2). J Biol Chem. 2009; 284:19402-11.
  13. Heenan EJ, Vanhooke JL, Temple BR, Betts L, Sondek JE, Dohlman HG. Structure and Function of Vps15 in the Endosomal G Protein Signaling Pathway. Biochemistry 2009; 48:6390-401.
  14. Ramachandran S, Temple BR, Chaney SG, Dokholyan NV. Structural basis for the sequence-dependent effects of platinum-DNA adducts. Nucleic Acids Res. 2009; 37(8):2434-48.


  15. Denofrio JC, Yuan W, Temple BR, Gentry HR, Parise LV. Characterization of calcium- and integrin-binding protein 1 (CIB1) knockout platelets: potential compensation by CIB family members. Thromb Haemost 2008; 100(5):847-856.
  16. Teotico DG, Frazier ML, Ding F, Dokholyan NV, Temple BRS, Redinbo MR. Active Nuclear Receptors Exhibit Highly Correlated AF-2 Domain Motions. PLOS Comp Biol 2008; 4(7):1-13.
  17. Marchese A, Paing MM, Temple BR, Trejo J. G Protein–Coupled Receptor Sorting to Endosomes and Lysosomes. Annu Rev Pharmacol Toxicol 2008; 48:601-29.
  18. Bowley SR, Merenbloom BK, Okumura N, Betts L, Heroux A, Gorkun OV, and Lord ST. Polymerization-defective fibrinogen variant gammaD364A binds knob “A” peptide mimic. Biochemistry, 2008; 47:8607–8613.
  19. Van Itallie CM, Betts L, Smedley JG 3rd, McClane BA, Anderson JM. Structure of the claudin-binding domain of clostridia perfringens enterotoxin. J. Biol. Chem. 2008; 283(1):268-74
  20. Charlton, Lisa M.; Barnes, Christopher O.; Li, Conggang; Orans, Jillian; Young, Gregory B.; Pielak, Gary J. Residue-Level Interrogation of Macromolecular Crowding Effects on Protein Stability. J Am Chem Soc 2008; 139:6826-6830.
  21. Li, Conggang; Charlton, Lisa M.; Lakkavaram, Asha; Seagle, Christopher; Wang, Guifang; Young, Gregory B.; Macdonald, Jeffrey M.; Pielak, Gary J. Differential Dynamical Effects of Macromolecular Crowding on an Intrinsically Disordered Protein and a Globular Protein: Implications for In-Cell NMR Spectroscopy. J Am Chem Soc 2008; 130:6310-6311.


  22. Johnston CA, Temple BR, Chen JG, Gao Y, Moriyama EN, Jones AM, Siderovski DP, Willard FS. Comment on "A G Protein–Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid". Science 2007; 318(5852):914.
  23. Sharma S, Gong P, Temple B, Bhattacharyya D, Dokholyan NV, Chaney SG. Molecular dynamic simulations of cisplatin- and oxaliplatin-d(GG) intrastand cross-links reveal differences in their conformational dynamics. J Mol Biol 2007; 373(5):1123-40.
  24. Brenman JE, Temple BRS. Opinion: Alternative Views of AMP-activated Protein Kinase. Cell Biochem Biophys 2007; 47(3):321-31.
  25. Wu Y, Bhattacharyya D, King CL, Baskerville-Abraham I, Huh SH, Boysen G, Swenberg JA, Temple B, Campbell SL, Chaney SG. Solution structures of a DNA dodecamer duplex with and without a cisplatin 1,2-d(GG) intrastrand cross-link: comparison with the same DNA duplex containing an oxaliplatin 1,2-d(GG) intrastrand cross-link. Biochemistry 2007; 46:6477-87.
  26. Ryan MM, Temple BRS, Phillips SE, Bankaitis VA. Conformational dynamics of the major yeast phosphatidylinositol transfer protein sec14p: insight into the mechanisms of phospholipid exchange and diseases of sec14p-like protein deficiencies. Mol Biol Cell 2007; 18(5):1928-42.
  27. Temple BR, Jones AM. The plant heterotrimeric G-protein complex. Ann Rev Plant Biol 2007; 58:249-66.
  28. Boysen G, Scarlett CO, Temple B, Combs TP, Brooks NL, Borchers CH, Swenberg JA. Identification of covalent modifications in P450 2E1 by 1,2-epoxy-3-butene in vitro. Chem Biol Interact 2007;166(1-3):170-5.
  29. Mitin N, Betts L, Yohe ME, Der CJ, Sondek J, Rossman KL. Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression. Nat. Struct. Mol. Biol. 2007; 14:814-823.
  30. Chhatriwala MK, Betts L, Worthylake DK, and Sondek J. The DH and PH Domains of Trio Coordinately Engage Rho GTPases for their Efficient Activation. J. Mol. Biol., 2007; 368:1307-1320.


  31. Slessareva JE, Routt SM, Temple B, Bankaitis VA, Dohlman HG. Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosomes. Cell 2006; 126(1):191-203.
  32. Beltran A, Liu Y, Parikh S, Temple B, Blancafort P. Interrogating genomes with combinatorial artificial transcription factor libraries: asking zinc finger questions. Assay Drug Dev Technol 2006; 4(3):317-331.
  33. Chen JG, Ullah H, Temple B, Liang J, Guo J, Alonso JM, Ecker JR, Jones AM. RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis. J Exp Bot 2006; 57(11):2697-708.
  34. Betts L, Merenbloom BK, and Lord ST. The structure of fibrinogen fragment D with the 'A' knob peptide GPRVVE. J Thrombosis and Haemostasis. 2006, 4:1139-1141.
  35. McNulty, BC, Young, GB, Pielak, GJ. 2006. Macromolecular crowding in the Escherichia Coli periplasm maintains alpha-synuclein disorder. Journal of Molecular Biology 355: 893-897.
  36. McNulty, BC, Tripathy, A, Young, GB, Charlton, LM, Orans, J, Pielak, GJ. 2006. Temperature-induced reversible conformational change in the first 100 residues of alpha-synuclein. Protein Science 15: 602-608.


  37. Morris SA, Shibata Y, Noma K, Tsukamoto Y, Warren E, Temple B, Grewal SI, Strahl BD. Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe. Eukaryot Cell 2005; 4(8):1446-54.
  38. Carl PL, Temple B, Cohen PL. Most nuclear systemic autoantigens are extremely disordered proteins: implications for the etiology of systemic autoimmunity. Arth Res & Therapy 2005; 7(6):R1360-74.
  39. Schaaf G, Betts L, Garrett TA, Raetz CR, Bankaitis VA. Crystallization and preliminary X-ray diffraction analysis of phospholipid-bound Sfh1p, a member of the Saccharomyces cerevisiae Sec14p-like phosphatidylinositol transfer protein family. Acta Cryst. Sect F Biol. Cryst. Commun. 2006; 62:1156-60.
  40. Uhlik MT, Temple B, Bencharit S, Kimple AJ, Siderovski DP, Johnson GL, Structural and evolutionary division of phosphotyrosine binding (PTB) domains. J Mol Biol 2005; 345(1):1-20.


  41. Gentry HR, Singer AU, Betts L, Yang C, Ferrara JD, Sondek J, Parise LV. Structural and biochemical characterization of CIB1 delineates a new family of EF-hand containing proteins. J Biol Chem. 2004; 280:8407-15.
  42. Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J. Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis. Structure 2004; 12:1669-81.
  43. Kostelansky MS, Bolliger-Stucki B, Betts L, Gorkun OV, Lord ST. B beta Glu397 and B beta Asp398 but not B beta Asp432 are required for "B:b" interactions. Biochemistry 2004; 43:2465-74.
  44. Chaney SG, Campbell SL, Temple B, Bassett E, Wu Y, Faldu M. Protein interactions with platinum-DNA adducts: from structure to function. J Inorg Biochem 2004; 98(10):1551-9.
  45. Paing MM, Temple BR, Trejo J. A tyrosine-based sorting signal regulates intracellular trafficking of protease-activated receptor-1: Multiple regulatory mechanisms for agonist-induced G protein-coupled receptor internalization. J Bio Chem 2004; 279:21938-47.
  46. Gidwitz S, Temple B, White II GC. Mutations in and near the second calcium binding domain of αIIb affect the structure and function of αIIbβ3. Biochemical Journal 2004; 379:449-59.
  47. Snider MG, Temple BS, Wolfenden R. The path to the transition state in enzyme reactions: a survey of catalytic efficiencies, J Phys Org Chem 2004; 17:586-591.


  48. Silversmith RE, Guanga GP, Betts L, Chu C, Zhao R, Bourret RB. CheZ-mediated dephosphorylation of the Escherichia coli chemotaxis response regulator CheY: role for CheY glutamate 89. J. Bacteriol. 2003; 185:1495-502.
  49. Ullah H, Chen JG, Temple B, Boyes DC, Alonso JM, Davis KR, Ecker JR, Jones AM, The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes, Plant Cell 2003; 15:393-409.


  50. Kostelansky MS, Betts L, Gorkun OV, Lord ST. 2.8 Å Crystal Structures of Recombinant Fibrinogen Fragment D with and without Two Peptide Ligands: GHRP Binding to the "b" Site Disrupts Its Nearby Calcium-binding Site Biochemistry 2002; 41:12124-32.
  51. Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J. Structural basis for the selective activation of Rho GTPases by Dbl exchange factors. Nat. Struct. Biol. 2002; 9:468-75.
  52. Dedmon, MM, Patel, CN, Young, GB & Pielak, GJ. 2002. FlgM gains structure in living cells. Proceedings of the National Academy of Sciences. U.S.A. 99: 12681-12684.
  53. Kimple RJ, Kimple ME, Betts L, Sondek J, Siderovski DP. Structural determinants for GoLoco-induced inhibition of nucleotide release by Ga subunits. Nature 2002 416:878-881.


  54. Morar, AS, Olteanu, A, Young, GB, Pielak, GJ. Solvent-induced collapse of α-synuclein and acid denatured cytochrome c. Protein Science 2001; 10: 2195-2199.


  55. Traut TW, Temple BRS, The chemistry of the reaction determines the invariant amino acids during the evolution and divergence of orotidine 5'-monophosphate decarboxylase, J of Biol Chem 2000; 275:28675-81.

    before 2000

  56. Gavin DK, Young Jr SM, Xiao W, Temple BRS, Abernathy CR, Pereira DJ, Muzyczka N, Samulski RJ, Charge-to-alanine mutagenesis of the adeno-associated virus type 2 Rep78/68 proteins yields temperature-sensitive and magnesium-dependent variants, J of Virology 1999; 73:9433-45.
  57. Morar, AS, Kakouras, D, Young, GB, Boyd, J, Pielak, GJ. Expression of 15N-labeled eukaryotic cytochrome c in Escherichia coli. Journal of Biological Inorganic Chemistry 1999; 4: 220-222.
  58. Wolfenden, RV, Ridgway, C, Young, GB. Spontaneous hydrolysis of ionized phosphate monoesters and diesters and the proficiencies of phosphatases and phosphodiesterases as catalysts. Journal of the American Chemical Society 1998; 120: 833-834.
  59. Wolfenden, RV, Lu, XD, Young, GB. Spontaneous hydrolysis of glycosides. Journal of the American Chemical Society 1998; 120: 6814-6815.
  60. Betz, SF, Marmorino, JL, Saunders, AJ, Doyle, DF, Young, GB, Pielak, GJ. Unusual effects of an engineered disulfide on global and local protein stability. Biochemistry 1996; 35: 7422-7428.
  61. Saunders, AJ, Young, GB, Pielak, GJ. Polarity of disulfide bonds. Protein Science 1993; 2: 1183-1184.
  62. Radzicka, A, Young, GB, Wolfenden, R. Lack of water transport by amino acid side chains or peptides entering a nonpolar environment . Biochemistry 1993; 32: 6807-6809.
  63. Marmorino, JL, Auld, DS, Betz, SF, Doyle, DF, Young, GB, Pielak, GJ. Amide proton exchange rates of oxidized and reduced Saccharomyces cerevisiae iso-1-cytochrome c. Protein Science 1993; 2: 1966-1974.
  64. Auld, DS, Young, GB, Saunders, AJ, Doyle, DF, Pielak, GJ. Probing weakly-polar interactions in cytochrome c. Protein Science 1993; 2: 2187-2197.





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