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Protein Expression and Purification Core Facility (PEP)
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PEP Director:
Michael Miley, Ph.D.
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Medical Research Building B
CB# 7365
tel: +1-919-843-7174

Instrumentation

General

Marketforge Sterilmatic autoclave Sorvall superspeed evolution RC centrifuge w/ fiberlite rotors (6x1L and 8x50mls)
Ice Machine
2 large -80C freezers
-20C freezer
2 Deli door 4C refrigerator
3 door deli 4C refrigerator (for FPLC)
NanoDrop 2000C
Gel documentation system (DNA/Protein)
Multiple Scales, heat blocks, stir-plates, microfuges, gel rigs (DNA/Protein) and a pH meter
Eppendorf 96well gradient PCR Machine
37C plate E. coli plate incubator
Small drying oven
Sorvall tabletop refrigerated centrifuge (ST 40R)
BioTek 96well plate washing robot (ELISAs)
4 foot wide hood

Protein Expression / Tissue Culture

2 Thermo MaxQ 8000 stackable temperature controlled shakers (E. coli)
Benchtop temperature controlled shaker for small scale E. coli cultures
Walk in 27C room outfitted with 2 shakers (MaxQ 2000/3000) and a 9 slot magnetic stirrer
2 Heracell 240 37C CO2 incubators
2 Heracell 37C CO2 incubators
1 Large Thermo Reach-in CO2 incubator outfitted with 2 Forma shakers
2 bio safety cabinets (4ft/6ft)
2 Olympus CKX31 inverted microscopes
Leica inverted microscope that is GFP fluorescence capability
BioRad automated cell counter

Purification

Millipore Tangential Flow Concentration device
2 GE AKTAxpress FPLCs
Amersham AKTA FPLC
Wide variety of pre-packed and gravity flow columns with a variety of resins

Conferences

Keystone Symposia – Frontiers of NMR in Biology
January 13-18, 2013
Snowbird, Utah

57th Biophysical Society Annual Meeting
February 2-6 2013
Philadelphia, PA

2013 Meeting of the American Crystallographic Association
July 20-24, 2013
Honolulu, Hawaii

54th Experimental Nuclear Magnetic Resonance Conference
April 14-19, 2013
Pacific Grove, CA

9th Annual Protein Engineering Summit (PEGS)
April 29th – May 3rd 2013
Boston, MA

Gordon Research Conference – Computational Aspects – Biomolecular NMR
June 2-7, 2013
Mount snow resort
West Dover, VT

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