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R.L. Juliano Structural Bioinformatics Core Facility (SBI)
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SBI Director:
Brenda Temple, Ph.D.
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4062 Genetic Medicine
CB# 7260
tel: +1-919-843-9399

Structural Bioinformatics Core Facility Current Research

 

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Welcome to the R. L. Juliano Structural Bioinformatics Core facility at the University of North Carolina at Chapel Hill. The Director of this Core, Dr. Brenda Temple, is available for consultations and collaborations on research studies requiring computational structural biology methods.

One effective analysis for the identification of functionally important amino acid residues combines 3D structural analysis with molecular evolution methods, including sequence and phylogenetic studies. When experimental structures are not available, molecular modeling studies, whereby the structure of the protein of interest is predicted using known template structures, provide 3D atomic data. The analyses available through this core are not limited to the study of static structures, but also include molecular dynamics studies for analyzing the contribution of dynamic and collective motions to macromolecular functionality. All of these analyses contribute to the development of hypotheses to be tested in the laboratory, and give insight into experimental data.

For more information on what services are available go to How We Work.

To learn about available software packages go to Resources.

For examples of current research using our tools, please visit Research Applications.


Latest Publications:

  1. Montgomery ER, Temple BR, Peters KA, Tolbert CE, Booker BK, Martin JW, Hamilton TP, Tagliatela AC, Smolski WC, Rogers SL, Jones AM, Meigs TE (2014).  Gα12 Structural Determinants of Hsp90 Interaction are Necessary for Serum Response Element-mediated Transcriptional Activation.  Mol Pharmacol 85(4):586-97.  PMID: 24435554

  2. Ren J, Pei-Chen Lin C, Pathak MC, Temple BR, Nile AH, Mousley CJ, Duncan MC, Eckert DM, Leiker TJ, Ivanova PT, Myers DS, Murphy RC, Brown HA, Verdaasdonk J, Bloom KS, Ortlund EA, Neiman AM, Bankaitis VA (2014).  A Phosphatidylinositol Transfer Protein Integrates Phosphoinositide Signaling With Lipid Droplet Metabolism To Regulate a Developmental Program of Nutrient Stress-Induced Membrane Biogenesis.  Mol Biol Cell 25(5):712-27.  PMID: 24403601

  3. Nile AH, Tripathi A, Yuan P, Mousley CJ, Suresh S, Wallace IM, Shah SD, Pohlhaus DT, Temple B, Nislow C, Giaever G, Tropsha A, Davis RW, St Onge RP, Bankaitis VA (2014). PITPs as targets for selectively interfering with phosphoinositide signaling in cells. Nat Chem Biol 10(1):76-84.  PMID: 24292071

  4. Chopra S, Palencia A, Virus C, Tripathy A, Temple BR, Velazquez-Campoy A, Cusack S, Reader JS (2013).  Plant tumor biocontrol agent employs a tRNA-dependent mechanism to inhibit leucyl-tRNA synthetase.  Nat Commun 4:1417. PMID: 23361008

  5. Bosch DE, Kimple AJ, Muller RE, Giguère PM, Machius M, Willard FS, Temple BR, Siderovski DP (2012).  Heterotrimeric G-protein signaling is critical to pathogenic processes in Entamoeba histolytica. PLoS Pathog. 8(11):e1003040. doi: 10.1371/journal.ppat.1003040.  PMID: 23166501

  6. Ramachandran S, Temple B, Alexandrova AN, Chaney SG, Dokholyan NV (2012).  Recognition of platinum-DNA adducts by HMGB1a.  Biochemistry. Sep 25;51(38):7608-17. doi: 10.1021/bi3008577.  PMID: 22950413

  7. Cherkis KA, Temple BR, Chung EH, Sondek J, Dangl JL (2012).  AvrRpm1 missense mutations weakly activate RPS2-mediated immune response in Arabidopsis thaliana.  PLoS ONE 7(8):e42633.  doi:10.1371/journal.pone.0042633  PMID:  22880057

  8. Lenz JD, Temple BR, Miller VL (2012). Evolution and virulence contributions of the autotransporter proteins YapJ and YapK of Y. pestis C092 and their homologs in Y. pseudotuberculosis IP32953. Infect Immun. Oct;80(10):3693-705.  PMID: 22802344

  9. Jones JC, Jones AM, Temple BRS, Dohlman, HG (2012).  Differences in intradomain and interdomain motion confer distinct activation properties to structurally similar Gα proteins.  PNAS. May 8;109(19):7275-7279

  10. Tomberg J, Temple B, Fedarovich A, Davies C, Nicholas RA (2012).  A highly conserved interaction involving the middle residue of the SXN active-site motif is crucial for binding proteins: mutational and computational analysis of PBP 2 from N. gonorrhoeae.  Biochemistry. Apr 3;51(13):2775-84.  PMCID:  PMC3338128

  11. Friedman EJ, Wang HX, Jiang K, Perovic I, Deshpande A, Pochapsky TC, Temple BRS, Hicks SN, Harden TK, Jones AM (2011).  Acidreductone dioxygenase 1 (ARD1) is an effector of the heterotrimer G protein beta subunit in Arabidopsis.  J Biol Chem. Aug 26;286(34):30107-18.  PMID:  21712381

  12. Hamel B, Monaghan-Benson E, Rojas RJ, Temple BRS, Marston DJ, Burridge K, Sondek J (2011).  SmgGDS is a guanine nucleotide exchange factor that specifically activates RhoA and RhoC.  J Biol Chem. Apr 8;286(14):12141-8.  PMCID: PMC3069418

  13. Jones JC, Duffy JW, Machius M, Temple BRS, Dohlman HG, Jones AM (2011).  The crystal structure of a self-activating G Protein α subunit reveals its distinct mechanism of signal initiation. Sci. Signal. 4, ra8.  PMID:  21304159

  14. Temple BR, Jones CD, Jones AM (2010).  Evolution of a signaling nexus constrained by protein interfaces and conformational states.  PLoS Comput Biol 6(10):e1000962.  PMCID: PMC2954821

 

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