Instrumentation

Please contact the Director, , to schedule use of NMR Laboratory Core Facility equipment and instrumentation. 

Bruker Avance II 850 Spectrometer

A Bruker Avance III 850 four channel spectrometer was installed in the facility in June 2014.  This spectrometer is equipped with a TCI H-C/N-D 5 mm CryoProbe. The probe has cryogenic preamps for direct observation of 1H and 13C at high sensitivity over a temperature range of -40C to 80C.  The spectrometer has a 24 slot sample changer.  It is primarily intended for studies of proteins and nucleic acids in solution.

The Inova 700 MHz Spectrometer

The Inova 700 MHz spectrometer is used for high resolution studies of liquid samples. It is equipped with a 5mm H1 {C13/N15} Cold Probe with Z-axis gradient for solvent suppression and coherence selection.  The spectrometer has four RF channels to allow simultaneous manipulation of H1, C13, N15, and H2 spins for studies of triple-labeled proteins. All RF channels are equipped with waveform generators to create shaped RF pulses. The sample temperature can be controlled to +/- 0.1 C in the range 0 to 50 C. The Inova 700 is used primarily for H1-detected studies of proteins and small molecules at low concentration.

The Inova 600 MHz Spectrometer

The Inova 600 MHz spectrometer is currently our general purpose spectrometer. The spectrometer has four RF channels to allow simultaneous manipulation of H1, C13, N15, and H2 spins for studies of triple-labeled proteins. Two of the RF channels are equipped with waveform generators to create shaped RF pulses. The spectrometer has three-axis gradients to allow solvent suppression and selection of coherence pathways. The sample temperature can be controlled to +/- 0.1 C. Three probes were purchased with the system: a 5mm 1H {C13/N15} X, Y, Z PFG triple resonance probe, and 8mm H1 {C13/N15} Z PFG triple resonance probe, and a 5mm N15 - P31 broadband probe with H1 decoupling.  The 8mm probe has been modified for use with a bioreactor to conduct studies of labeled proteins in living cells.

The Inova 500 MHz Spectrometer

The Inova 500 MHz spectrometer is used for high resolution studies of liquid samples. It is equipped with a 5mm H1 {C13/N15} Cold Probe with Z-axis gradient for solvent suppression and coherence selection.  The spectrometer has four RF channels to allow simultaneous manipulation of H1, C13, N15, and H2 spins for studies of triple-labeled proteins. Two of the RF channels are equipped with waveform generators to create shaped RF pulses. The sample temperature can be controlled to +/- 0.1 C in the range 0 to 50 C. The Inova 500 is used primarily for H1-detected studies of proteins and small molecules at low concentration.

Lower Resolution Spectrometers

For samples that do not require a high-field spectrometer, the Chemistry department has several lower field spectrometers. Contact Marc TerHorst (terhorst@unc.edu) for information.