Instrumentation

Please contact core director, Michael J. Miley, to schedule use of Protein Expression and Pufification Core Facility equipment and instrumentation.

General

  • Marketforge Sterilmatic autoclave Sorvall superspeed evolution RC centrifuge w/ fiberlite rotors (6x1L and 8x50mls)
  • Ice Machine
  • 2 large -80C freezers
  • -20C freezer
  • 2 Deli door 4C refrigerator
  • 3 door deli 4C refrigerator (for FPLC)
  • NanoDrop 2000C
  • Gel documentation system (DNA/Protein)
  • Multiple Scales, heat blocks, stir-plates, microfuges, gel rigs (DNA/Protein) and a pH meter
  • Eppendorf 96well gradient PCR Machine
  • 37C plate E. coli plate incubator
  • Small drying oven
  • Sorvall tabletop refrigerated centrifuge (ST 40R)
  • BioTek 96well plate washing robot (ELISAs)
  • 4 foot wide hood

Protein Expression / Tissue Culture

  • 2 Thermo MaxQ 8000 stackable temperature controlled shakers (E. coli)
  • Benchtop temperature controlled shaker for small scale E. coli cultures
  • Walk in 27C room outfitted with 2 shakers (MaxQ 2000/3000) and a 9 slot magnetic stirrer
  • 2 Heracell 240 37C CO2 incubators
  • 2 Heracell 37C CO2 incubators
  • 1 Large Thermo Reach-in CO2 incubator outfitted with 2 Forma shakers
  • 2 bio safety cabinets (4ft/6ft)
  • 2 Olympus CKX31 inverted microscopes
  • Leica inverted microscope that is GFP fluorescence capability
  • BioRad automated cell counter

Purification

  • Millipore Tangential Flow Concentration device
  • 2 GE AKTAxpress FPLCs
  • Amersham AKTA FPLC
  • Wide variety of pre-packed and gravity flow columns with a variety of resins