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Center for Structural Biology

Instrumentation

Please contact core Director to schedule use of Protein Expression and Purification Core Facility equipment and instrumentation.

General

  • Marketforge Sterilmatic autoclave Sorvall superspeed evolution RC centrifuge w/ fiberlite rotors (6x1L and 8x50mls)
  • Ice Machine
  • 2 large -80C freezers
  • 2 -20C freezers
  • 2-bay 4C refrigerator
  • 2 3-bay 4C refrigerators (for FPLCs)
  • NanoDrop One spectrophotometer
  • Gel scanner documentation system (DNA/Protein)
  • Multiple Scales, heat blocks, stir-plates, microfuges, gel rigs (DNA/Protein), and a pH meter
  • Eppendorf 96 well gradient-capable PCR Machine
  • 37C plate E. coli plate incubator
  • Small drying oven
  • Sorvall tabletop refrigerated centrifuge (ST 40R)
  • Chemical fume hood

Protein Expression / Tissue Culture

  • 2 Thermo MaxQ 8000 stackable temperature controlled shakers (E. coli)
  • Benchtop temperature controlled shaker for small scale E. coli cultures
  • 2 Heracell 240 37C CO2 incubators
  • 2 Heracell 37C CO2 incubators
  • 1 Large Thermo Reach-in CO2 incubator outfitted with 2 Forma shakers
  • 4 biological safety cabinets (4ft/6ft)
  • 2 Olympus CKX31 inverted microscopes
  • Leica inverted microscope with fluorescence
  • BioRad automated cell counters

Purification

  • Millipore Tangential Flow Concentration device
  • 2 Cytiva AKTAxpress FPLCs
  • Cytiva Pure FPLC
  • Cytiva AktaStart FPLC
  • Wide variety of pre-packed and gravity flow columns with a variety of resins