The UNC Flow Cytometry Facility is available to all UNC-CH investigators. The Facility is also available to investigators outside UNC - Duke, NC State and companies in the RTP. Contract work with institutions other than those above can be accommodated. Access is mostly based on a first-come, first-served basis.
When publishing, please include the following statement in your acknowledgements: The UNC Flow Cytometry Core Facility is supported in part by an NCI Center Core Support Grant (P30CA06086) to the UNC Lineberger Comprehensive Cancer Center
Analytical Instruments - The non-sorting, analysis-only flow cytometers are available for use by investigators or their staff in two ways. First, the Facility staff will operate the instruments for investigators. We require that the investigator remain at the instrument while we run the samples so we may consult if questions arise. If we run the same type samples repeatedly for an investigator we can operate in a "drop off the samples mode". Second, the investigator may operate the instrument. This is provided at a reduced cost. However, each user is required to take our training class (see "Training"). Training is required for each different type of instrument you wish to use. Users are not permitted to be trained by other members of their lab - only by Facility staff. As a rule of thumb, we do not recommend that you take the training class and operate the instruments unless you plan on doing a substantial amount of use. If you use the instrument infrequently your data quality will very likely suffer. The training course includes some individual help sessions to get you started. To use the instruments users may schedule their own time (unless it requires Facility staff participation) - (see "Instrument Schedules").
Sorting - The sorting instruments are available only as run by the Facility staff. Please call (6-1530) to make an appointment. Please download and fill out the "Sort Request Form" and after completing please FAX it to us so we may confirm your appointment. The MoFlo and MoFlo XDP are not available for sorting biohazard samples. For these samples please plan to use the Reflection instrument. We must have on file a current "Biosafety Form". This form needs to be submitted each time a sort is requested (note - you may resubmit the same form if there are no changes). Call to schedule time, then fax or email the sort request form. We require a copy of your SCHEDULE F for any biohazardous materials brought into the core. See also our 'Sorting Tips' under our Basic Flow Cytometry page: https://www.med.unc.edu/flowcytometry/basic-flow-cytometry/practical-cell-sorting-a-guide-for-investigators
Consultation - The Facility Director is available for consultation on any component and aspect of flow cytometry. Please call or email for an appointment.
First time users overview:
- Read the web site for rules and overview of flow cytometry.
- Read appropriate protocols.
- Talk to Facility Director or staff about your needs and whether we can do what you want.
- Call Facility for an appointment (6-1530). We will run your samples until you are trained (see Training).
- Bring samples to Facility for analysis. Observe samples being run to familiarize yourself.
- Analyze data.
- Write your paper - get famous!!