Fluidigm Helios CyTOF

Anticipated availability at UNC Chapel Hill in the Flow Cytometry Core Facility later in the Spring, 2018

The Fluidigm Helios CyTOF is a mass cytometer that uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. 

The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. Cellular targets in liquid suspension can be labeled with more than 40 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands.

Targets can be anywhere in the cell, including the surface, cytoplasm, nucleus or internal organelles. Complete profiling allows researchers to investigate a range of applications, including phenotyping, functional activity, apoptosis and cell cycle analysis. Phosphoprotein detection can be used to map the signaling profile of samples.

Resources 

Specifications:

Channels135
Mass range 75–209 amu
Abundance sensitivity 0.3% for 159Tb
Instrument response 600,000 counts/pg 159Tb
Detection limit 350 antibodies/cell
Dynamic range 4.5 orders of magnitude
Sample introduction Pneumatic single tube loader with agitation, up to 5 ml volume
Peak throughput 2,000 (events/sec)
Flow rate 30–45 (µL/min) 
Replicate sample CV (normalized) <3%
Data File Size (IMD) 0.3 MB/sec/channel
Data File Size (FCS) 12.4 bytes/event/channel
Data File Size (TXT) 2.4 bytes/event/channel

Helios CyTOFHelios CyTOF Metals

Located in Marsico Building - Room B022

Scheduling