Protocols

Cell Cycle:
Propidium Iodide Staining
Analysis of Cellular DNA Content by Flow and Laser Scanning Cytometry

Antibody Titration

DNAse to Prevent Cell Clumping

Cell Filtration Options

Fixation

Live/Dead Cell Discrimination

BioLegend Mitospy mitochondria stains in orange and green

RNA Extraction From Sorted Cells

Rainbow Calibration Particles (we encourage all analyzer users to perform their own quality control! We recommend each lab have available some Spherotech 8-peak Rainbow Calibration beads, Cat # RCP-30-5A)