Services

Services offered by the Functional Genomics Core are listed below. Please contact the Director for pricing information.

Gene 2.1 ST Peg Plates:

One sample goes on one peg array. This service includes sample preparation which involves a cDNA reaction, an IVT reaction to linearly amplify and label the RNA and clean up of the sample.  This is followed by hybridization, washing and scanning of the array with basic data analysis using Expression Console software. In addition, a copy of all files will always be stored here at the facility. The investigator is to provide the Affymetrix peg array, Ambion WT Expression Kit for High Throughput robotics, and Affymetrix Terminal Labeling Kit.
Expression 16 Peg Array Submission Sheet
Expression 24 Peg Array Submission Sheet
Expression 96 Peg Array Submission Sheet

 

Gene 2.0 ST Arrays:

One sample goes on one array.  Service includes sample preparation which involves a cDNA reaction, an IVT reaction to linearly amplify and label the RNA and clean up of the sample. This is followed by hybridization, washing and scanning of the array with basic data analysis using Expression Console software. In addition, a copy of all files will always be stored here at the facility.
Gene Array Submission Sheet

 

Axiom Genotyping Peg Plates:

Per plate charge for the sample preparation, hybridization wash and scan of the plate.  Each plate consists of 96 arrays.  The investigator is to provide the Axiom 2.0 reagent kit, peg plate and samples.
Axiom Whole Genome Mapping Arrays Submission Sheet
Axiom Whole Genome Mapping Arrays Submission Sheet - Non UNC


Expression 3’ IVT Arrays:

One sample goes on one array. This service includes sample preparation which involves a cDNA reaction, an IVT reaction to linearly amplify and label the RNA and clean up of the sample. This is followed by hybridization, washing and scanning of the array with basic data analysis using Expression Console software. In addition, a copy of all files will always be stored here at the facility.  The investigator is to provide the cartridge array.
Expression Arrays Submission Sheet
Expression Arrays Submission Sheet - Non UNC

 

Mapping Arrays:

Service includes hybridization, washing and scanning of a SNP genotyping array. The investigator is expected to provide the facility with a prepared hybridization cocktail ready to be put on an array along with the arrays.
Whole Genome Mapping Arrays Submission Sheet

 

Gene Regulation Arrays:

Per array for hybridization, washing and scanning of a gene regulation array. The investigator is expected to provide the facility with a prepared hybridization cocktail ready to be put on an array.  GeneChip® Tiling Arrays are the most comprehensive array tools available for discovering novel RNA transcripts or mapping sites of protein/DNA interaction in chromatin immunoprecipitation experiments.
Gene Regulation Arrays Submission Sheet