The Genomics Core has hybridized thousands of microarrays and prepared thousands of RNA libraries for UNC researchers. We have the experience and expertise to bring accuracy and consistency to your genomic assays.
Microarray services: All methods use fluoresce Cy-dye labeling and long oligo (60 mer) Agilent high density microarrays
• mRNA expression
2 colors Cy-dyes separately labeled (amplified) sample and reference hybridize on a same microarray.
1 color Cy-dye labeled (amplified) sample or reference hybridize on each microarray.
1 color Cy-dye labeled small RNA sample hybridized on each microarray printed with 20-40 repeats of each microRNA feature.
• Gene dosage using comparative genomic hybridization (CGH)
Using direct labeling method, 2 color separately labeled sample genomic DNA and reference genomic DNA hybridize on a same microarray.
Library preparation services for high throughput sequencing:
• From initial total RNA to final mRNA library
• From initial total RNA (include total RNA isolated from FFPE tissue) to final total strand RNA library
• From initial total RNA (include total RNA isolated from FFPE tissue) to final small RNA library
Both microarray and RNA-seq are powerful methods to analyze the transcriptome at a given point in time. While microarrays are less expensive, faster, and easier to interpret, sequencing of mRNA has the important advantage of providing unbiased information about the entire transcriptome, which can be used to determine mutations, splice variants, gene fusions and other unanticipated events, though at a substantially higher cost. Both techniques are highly useful and the choice should depend on the exact, the goals of the research. Please consult with the lab when planning your study so that we can assure that you are using the best approach to meet your objectives.
Example applications include:
• Differences in normal and cancer tissues
• Deregulated cellular pathways following experimental treatment
• Genetic pathways (e.g. knock-out mouse)