Prices Charged For Library Preparation:

•  mRNA Library Preparation: $175 per library prepared. mRNA Libraries can be processed by automation with 24, 32, 40, and 48 samples. The prices are $170/Library for run 24 samples, $165/Library for run 32 samples, $160/Library for run 40 samples, $155/Library for run 48 samples. More than 48 samples will charge by combination. For example, 56 samples will run 2 sets (32 and 24); 50 samples will run a set 48 and 2 individual manually prep.

•  Small RNA library preparation: Individual libraries with unique indices may be pooled (max 8 libraries) and gel purified together. $400, $380, $360,......$260/library for 1, 2, 3,......8 mixed libraries with pooled and combined gel purification method. More than 8 samples will charge by combination.

•  Total (FFPE) RNA library preparation: $240 per library prepared. Total RNA Libraries can also be processed by automation with 24, 32, 40, and 48 samples. The prices are $230/Library for run 24 samples, $220/Library for run 32 samples, $210/Library for run 40 samples, $200/Library for run 48 samples. More than 48 samples will charge by combination. For example, 56 samples will run 2 sets (32 and 24); 50 samples will run a set 48 and 2 individual manually prep.

Prices Charged For Microarray Analyses:

Gene Expression Microarrays

The charge for each sample for labeling, hybridization, scanning of arrays, and quality analysis of data based on spiked-in controls when available:

Lineberger Cancer Center Members:

•  1 color label method, using 4-array chip: $127/sample

•  2 color label method, using 4-array chip: $211/sample(4 samples with same reference), $288/sample (4 samples, each with different reference)

•  1 color label method, using 8-array chip: $113/sample

•  2 color label method, using 8-array chip: $185/sample (8 samples with same reference), $274/sample (8 samples, each with different reference)

All other UNC investigators using a UNC grant number:

•  1 color label method, using 4-array chip: $148/sample

•  2 color label method, using 4-array chip: $247/sample (4 samples with same reference), $337/sample (4 samples, each with different reference)

•  1 color label method, using 8-array chip: $132/sample

•  2 color label method, using 8-array chip: $216/sample (8 samples with same reference), $321/sample (8 samples, each with different reference)

MicroRNA Microarrays

The charge of Genomics Core for each sample labeling and hybridization:

•  For Lineberger Member: $164/sample

•  For all others use UNC grant number: $197/sample

CHG Microarrays

The charge of Genomics Core for each sample labeling and hybridization:

•  For Lineberger Member: $179/sample (if use 4-array chip), $247/sample (if use 1-array chip)

•  For all others use UNC grant number: $215/sample (if use 4-array chip), $298/sample (if use 1-array chip)

Prices Charged for Quality and Concentration Measurements of RNA and DNA:

NanoDrop and Qubit

RNA and DNA concentrations are measured by Nanodrop Spectrophotometer and by Qubit Fluorometer.

•  Price for using Nanodrop: $2 per measurement

•  Price for using Qubit (only for dsDNA): $4 per measurement

Bioanalyzer and TapeStation

RNA and DNA size and integrity are measured by electrophoresis using the Agilent Bioanalyzer 2100 or Agilent TapeStation 2200. The Bioanalyzer chips analyze from 1 to 11, or 12 samples depending on using pico or nano chip. The chips are consumed by the measurement. Thus the cost of measuring is $42/chip.

The TapeStation tapes analyze from 1 to 16. Since it run a pair channel each time (2, 4, 6, 8 and so on) and the unused channels on tape can be used in next 10 days. The charge for sample # less than 8 will be $10 for 1-2 samples, $20 for 3-4 samples, $30 for 5-6 samples, and $40 for 7-8 samples. The charge for samples # 9-16 is $60 per tape. The sample # >16 will charge by combination.