Feb 13, 2014
from 04:30 PM to 05:30 PM
|Where||1131 Bioinformatics Building|
|Contact Name||Fern Jeremiah|
|Add event to calendar||
CGIBD Research Seminar
"Oral Nanotherapeutics: Promise and Challenge"
Kam W. Leong, Ph.D.
James B. Duke Professor
Departments of Biomedical Engineering, and Surgery
Many promising new drug candidates ensuing from genomic and proteomic revolutions can now target diseases at the molecular level. However, many of these therapeutic entities, often peptide and nucleic acid in nature, require an effective intracellular delivery system to exert their effect. Nanoparticle- and biomaterials-mediated delivery represents a logical solution. If such drug-loaded devices can be effectively administered orally to reach their intended intracellular targets, the appeal of this future generation of peptide- and DNA-based therapeutics would even be more significant. This presentation will focus on polymeric gene carriers, which enjoy the advantage of versatility and relative biocompatibility. I will review our studies on using chitosan as a carrier to deliver FVIII and FIX genes for hemophilia therapy via the oral route. This is complemented by studies to understand the rate barriers in nonviral gene transfer using quantum dot-FRET (QD-FRET) technology, which leads to the use of bioreductive polycations for more effective intracellular delivery. Finally, if time allows I will also cover our recent effort on applying microfluidics to improve the self-assembly of polycation-DNA nanocomplexes.