Biosketch

Biosketch

Personal Information:

 

Name: Dennis A. Simpson

 

Email:

dennis@email.unc.edu

Phone:

(919) 357-1028 (Cell)

(919) 966-8552 (Lab)

 

Education – Postgraduate Training Fellowships, Residencies and Traineeships

Degree            Institution                         Date                              Field

University of North Carolina    1998-present   DNA Damage/Cell Cycle Checkpoints

University of North Carolina    1996-1998       Biology of Fanconi Anemia

Ph.D.   University of North Carolina    1988-1996       Microbiology and Immunology

B.S.     Michigan State University       1981-1985       Microbiology and Public Health

 

Employment History:              (Begin with current position, rank and date of appointment)

Research Assistant Professor             2007    Dept. of Pathology and Lab. Medicine

University of North Carolina

Research Instructor                             2002    Dept. of Pathology and Lab. Medicine

University of North Carolina

Postdoctoral Research Associate       2000    Dept. of Pathology and Lab. Medicine

University of North Carolina

Postdoctoral Research Associate       1998    Dept. of Pathology and Lab. Medicine

University of North Carolina

Postdoctoral Research Fellow                        1996    Gene Therapy Center

University of North Carolina

Research Assistant                             1985    Dept. of Microbiology and Public Health

Michigan State University

Honors and Awards:

Visiting Scholar, University of Ulster, Colerain, Northern Ireland.  (2000)

Postdoctoral Research Associate, Environmental Pathology, UNC-CH. (2000-2001)

Postdoctoral Fellowship, University of North Carolina at Chapel Hill, Curriculum in Toxicology.  (1998-2000)

Postdoctoral Fellowship, University of North Carolina at Chapel Hill Gene Therapy Center. (12/96 – 6/98)

Graduate Training Fellowship: Design of Genetically Engineered Viral Vaccines, U.S. Army Research Office.  (8/92 – 12/96)

 

Bibliography

 

Refereed Publications:

  1. Smith‐Roe, S. L., Patel, S. S., Zhou, Y. C., Simpson, D. A., Rao, S., Ibrahim, J. G., Cordeiro‐Stone, M., Kaufman, W. K. (2012) Cell Cycle 12. [Epub ahead of print]
  2. Kesseler, K.J., Blinov, M.L., Elston, T.C., Kaufmann, W.K., Simpson, D.A. (2012) Journal of Theoretical Biology doi:10.1016/j.jtbi.2012.12.011. [Epub ahead of print]
  3. Carson C, Omolo B, Chu H, Zhou Y, Sambade MJ, Peters, E. C., Tompkins, P., Simpson, D. A., Thomas, N. E., Fan, C., Sarasin, A., Dessen, P., Shields, J. M., Ibrahim, J. G., Kaufmann, W. K.

(2012) Pigment cell & melanoma research 25: 514-526.

  1. Jeffries CD, Johnson CR, Zhou T, Simpson DA, Kaufmann WK (2012) Gene Regul Syst Bio 6: 55-66.
  2. Smith-Roe, S. L., Patel, S. S., Simpson, D. A., Zhou, Y. C., Rao, S., Ibrahim, J. G., Kaiser-Rogers, K. A., Cordeiro-Stone, M., and Kaufmann, W. K. (2011) Cell cycle 10, 1618-1624
  3. Bower, J. J., Zhou, Y., Zhou, T., Simpson, D. A., Arlander, S. J., Paules, R. S., Cordeiro-Stone, M., and Kaufmann, W. K. (2010) Cell cycle 9, 1617-1628
  4. Bower, J. J., Karaca, G. F., Zhou, Y., Simpson, D. A., Cordeiro-Stone, M., and Kaufmann, W. K. (2010) Oncogene 29, 4787-4799
  5. Tran, N., Qu, P. P., Simpson, D. A., Lindsey-Boltz, L., Guan, X., Schmitt, C. P., Ibrahim, J. G., and Kaufmann, W. K. (2009) Cell biochemistry and biophysics 53, 101-114
  6. Chen, B., Simpson, D. A., Zhou, Y., Mitra, A., Mitchell, D. L., Cordeiro-Stone, M., and Kaufmann, W. K. (2009) Cell Cycle 8, 1775-1787

10.  Kaufmann, W. K., Nevis, K. R., Qu, P., Ibrahim, J. G., Zhou, T., Zhou, Y., Simpson, D. A., Helms-Deaton, J., Cordeiro-Stone, M., Moore, D. T., Thomas, N. E., Hao, H., Liu, Z., Shields, J. M., Scott, G. A., and Sharpless, N. E. (2008) J Invest Dermatol 128, 175-187

11.  Zhou, T., Chou, J., Zhou, Y., Simpson, D. A., Cao, F., Bushel, P. R., Paules, R. S., and Kaufmann, W. K. (2007) Mol Cancer Res 5, 813-822

12.  Zhou, T., Chou, J., Mullen, T. E., Elkon, R., Zhou, Y., Simpson, D. A., Bushel, P. R., Paules, R. S., Lobenhofer, E. K., Hurban, P., and Kaufmann, W. K. (2007) Cell cycle 6, 972-981

13.  Heffernan, T. P., Unsal-Kacmaz, K., Heinloth, A. N., Simpson, D. A., Paules, R. S., Sancar, A., Cordeiro-Stone, M., and Kaufmann, W. K. (2007) The Journal of biological chemistry 282, 9458-9468

14.  Cao, F., Zhou, T., Simpson, D., Zhou, Y., Boyer, J., Chen, B., Jin, T., Cordeiro-Stone, M., and Kaufmann, W. (2007) Toxicol Appl Pharmacol 218, 174-185

15.  Zhou, T., Chou, J. W., Simpson, D. A., Zhou, Y., Mullen, T. E., Medeiros, M., Bushel, P. R., Paules, R. S., Yang, X., Hurban, P., Lobenhofer, E. K., and Kaufmann, W. K. (2006) Environmental health perspectives 114, 553-559

16.  Kaufmann, W. K., Filatov, L., Oglesbee, S. E., Simpson, D. A., Lotano, M. A., McKeen, H. D., Sawyer, L. R., Moore, D. T., Millikan, R. C., Cordeiro-Stone, M., and Carey, L. A. (2006) Carcinogenesis 27, 2519-2527

17.  Simpson, D. A., Livanos, E., Heffernan, T. P., and Kaufmann, W. K. (2005) Journal of carcinogenesis [electronic resource] 4, 18

18.  King, N. M., Nikolaishvili-Feinberg, N., Bryant, M. F., Luche, D. D., Heffernan, T. P., Simpson, D. A., Hanaoka, F., Kaufmann, W. K., and Cordeiro-Stone, M. (2005) DNA Repair (Amst) 4, 714-724

19.  Kaufmann, W. K., Heffernan, T. P., Beaulieu, L. M., Doherty, S., Frank, A. R., Zhou, Y., Bryant, M. F., Zhou, T., Luche, D. D., Nikolaishvili-Feinberg, N., Simpson, D. A., and Cordeiro-Stone, M. (2003) Mutat Res 532, 85-102

20.  Heise, M. T., White, L. J., Simpson, D. A., Leonard, C., Bernard, K. A., Meeker, R. B., and Johnston, R. E. (2003) Journal of virology 77, 1149-1156

21.  Doherty, S. C., McKeown, S. R., McKelvey-Martin, V., Downes, C. S., Atala, A., Yoo, J. J., Simpson, D. A., and Kaufmann, W. K. (2003) J Natl Cancer Inst 95, 1859-1868

22.  Mauser, A., Holley-Guthrie, E., Simpson, D., Kaufmann, W., and Kenney, S. (2002) Journal of Virology 76, 10030-10037

23.  Kaufmann, W. K., Campbell, C. B., Simpson, D. A., Deming, P. B., Filatov, L., Galloway, D. A., Zhao, X. J., Creighton, A. M., and Downes, C. S. (2002) Cell Cycle 1, 210-219

24.  Heffernan, T. P., Simpson, D. A., Frank, A. R., Heinloth, A. N., Paules, R. S., Cordeiro-Stone, M., and Kaufmann, W. K. (2002) Mol Cell Biol 22, 8552-8561

25.  Heise, M. T., Simpson, D. A., and Johnston, R. E. (2000) Journal of virology 74, 4207-4213

26.  Heise, M. T., Simpson, D. A., and Johnston, R. E. (2000) Journal of virology 74, 9294-9299

27.  Walsh, C. E., Yountz, M. R., and Simpson, D. A. (1999) Biochem Biophys Res Commun 259, 594-599

28.  Simpson, D. A., Davis, N. L., Lin, S. C., Russell, D., and Johnston, R. E. (1996) Virology 222, 464-469

29.  McKnight, K. L., Simpson, D. A., Lin, S. C., Knott, T. A., Polo, J. M., Pence, D. F., Johannsen, D. B., Heidner, H. W., Davis, N. L., and Johnston, R. E. (1996) Journal of Virology 70, 1981-1989

30.  Simpson, D. A., Hausinger, R. P., and Mulks, M. H. (1988) Journal of bacteriology 170, 1866-1873

 

Presented Papers

1.        Simpson, D.A., and Kaufmann, W.K. Ectopic Expression of Telomerase (hTERT) Prevents Chromosomal Destabilization in Cells Lacking Wild-Type p53 Function.  Salk Institute Cell Cycle Meeting, 2001.

2.        Heise M. T., Simpson, D.A., White, L. J., Ryman, K. D., Klimstra W. B., Beranrd K.  A., and Johnston R. E. Adult mouse neurovirulence determinants within the nonstructural genes of the Sindbis-group alaphavirus, S.A.AR86.  American Society for Virology.  2000.

3.        Heise, M. T., Simpson D.A., White, L. J., Ryman, K. D. Klimstra W. B., Bernard, K.  A., Johnston R. E. Adult mouse neurovirulence determinants within the nonstructural genes of the Sindbis-group alaphavirus, S.A.AR86.  Sixth Southeastern Regional Virology Conference, Atlanta.  2000.

4.        Heise M. T., Simpson D.  A., and Johnston R. E. The role of viral nonstructural genes in neurovirulence of the Sinbis-group virus, S.A.AR86.  Keystone Symposium, Cell Biology of Virus Entry, Replication and Pathogenesis.  2000.

5.        Heise, M. T., Simpson, D. A., and Johnston R. E. A single amino acid change within nsP1 of Sindbis-group viruses alters neurovirulence in adult mice.  American Society for Virology.  1999.

6.        Johnston R. E., MacDonald G. H., Heise M. T., Simpson D. A., Bernard K. A., Richmond E. M. B., Caley I. J., Swanstrom R., Johnson P., Brown K. W., and Davis N. L. In vivo gene expression from alphavirus-based replicons.  Keystone Symposium, DNA Vaccines:  Immune Responses, Mechanisms, and Manipulating Antigen Processing.  1999.

7.         Simpson, D. A., Olsen J., and Kaufmann W. K. Expression of hTERT sustains G2 checkpoint function in human fibroblasts expressing HPV-16 E6 oncoprotein.  Keystone meeting.  1999.

  1. Heise M. T., Simpson D. A. and Johnston R. E. Replication within bone/joint tissue of adult mice by S.A.AR86, a Sindbis group virus associated with arthralgia in humans.  American Society for Virology, 1998.
  2. Heise M. T., Simpson D. A. and Johnston R. E. Replication within bone/joint tissue of adult mice by S.A.AR86, a Sindbis group virus associated with arthralgia in humans.  Fifth International Symposium on Positive Strand RNA viruses, 1998.
  3. Haberman R. P., Simpson D. A., Walsh C. E., McCown T. J. and Samulski R. J. Tetracycline regulated green fluorescent protein (GFP) expression in vitro using adeno-associated virus (AAV) gene transfer.  Society for Neuroscience 27th Annual Meeting, 1997.
  4. Simpson D. A., and Walsh C. E. Phenotypic correction correlates with nuclear localization of Fanconi Anemia Group A protein. American Society of Hematology 39th Annual Meeting, 1997.
  5. Simpson D. A., Russell D. L., Lin S.- C., and Johnston R. E. Characterization and cloning of a naturally occurring Sindbis virus isolate which is not restricted in its neurovirulence by the age of the mouse. Southeast Regional Virology Conference, 1996.
  6. Simpson D. A., Russell D. L., Lin S.- C., and Johnston R. E. Characterization and cloning of S.A.AR86, a naturally occurring Sindbis virus isolate which is not restricted in its neurovirulence by the age of the mouse. Fourth International Symposium on Positive Strand RNA viruses, 1995.
  7. Simpson D. A., Russell D. L., Lin S.- C., and Johnston R. E. Construction of an infectious clone of Sindbis isolate S.A.AR86. Southeast Regional Virology Conference 1994.
  8. Simpson D. A., Russell D. L., Lin S.- C., and Johnston R. E. Molecular cloning and sequence analysis of alphavirus S.A.AR86. The American Society for Virology 12th Annual Meeting, 1993.
  9. Mulks M., Simpson D. A., and Shoberg R. J. Restriction site polymorphism in genes encoding Type 2 but not Type 1 gonococcal IgA1 proteases. Proceedings of the 5th International Pathogenic Neisseria Conference, 1988.
  10. Simpson D., Hausinger R. and Mulks M. Purification, characterization, and comparison of gonococcal IgA1 proteases. Proceedings of the 5th International Pathogenic Neisseria Conference, 1986.

Proceedings Journals

1.        Mulks M., Simpson D. A. and Shoberg R. Restriction site polymorphism in genes encoding Type 2 but not Type 1 gonococcal IgA1 protease.  Antonie van Leeuwenhoek J. of Microbiology 53:471-478, 1987.

Patents

  1. System for the in vivo delivery and expression of heterologous genes in the bone marrow.  R. E. Johnston, N. L. Davis, and D. A. Simpson.  (1998). Patent number 5,811,407.
  2. System for the in vivo delivery and expression of heterologous genes in the bone marrow.  R. E. Johnston, N. L. Davis, and D. A. Simpson.  (1999). Patent number 6,008,035.
  3. cDNA clone for South African Arbovirus No. 86.  R. E. Johnston, D. A. Simpson, and N. L. Davis (1997).  Patent number 5,639,650.
  4. System for the in vivo delivery and expression of heterologous genes in the bone marrow.  R. E. Johnston, D. A. Simpson, and N. L. Davis (2003).  Patent number 6,583,121.
  5. Vectors Derived from South African Arbovirus No. 86. D.A. Simpson, M.T. Heise, and R.E. Johnston. (2006). Patent number 6,982,087.

 

Teaching record .

Directly supervise and assist undergraduate honors students in project design, experimental implementation, and data interpretation.  Also directly supervise two work study students.  Work study students taught proper lab safety and were given a semi-independent project to measure the cytotoxicity of ultraviolet light on human melanocytes. Oversee technicians and graduate students teaching lab safety and experimental design.  Responsible for teaching approximately 30 graduate students, post-docs, and principle investigators how to use an X-ray source and how to design experiments for the X-ray source.

 

Grants

 

P01 ES014635-05                   Kaufmann (PI)                                    09/10/07-04/30/12

NIH/NIEHS

The System of Response to DNA Damage Suppresses Environmental Melanomagenesis

This program project investigates the mechanisms whereby UV-induced DNA damage causes melanoma. Three research projects will determine how various systems of DNA damage response protect human and murine melanocytes from the chromosomal damage that is induced by UV light. Studies will determine whether mutations in oncogenes and tumor suppressor genes that are known to occur in melanomas affect melanocyte responses to DNA damage. The program is supported by an administrative core, a cell and molecular biology core and a biostatistics and data management core. Dr. Simpson is co-investigator on projects 1 and 2 as well as core investigator on the Cell Biology core and the Biostatistics and Computational Biology core.

 

3-10108           Simpson (PI)                                                   12/2009 – 11/2011

University Research Council, University of North Carolina

Testing Predictions of a Novel Mathematical Model Describing the G2 to Mitosis Transition in Normal Human Cells.

The goal of this study was to test biological predictions of a G2 checkpoint model thus validating the model.

 

Professional Service

Manuscript referee for PLoS   2012 – Present

 

Reflective Statement

As a researcher I am driven by both a strong curiosity and by the challenge of understanding how biological systems function.  This is ultimately what led me to pursue a career as a researcher.  I have over 20 years of experience with laboratory research with 10+ year of experience conducting independent research and managing laboratory operations.  During the past five years my research has focused on potential early steps in the progression of normal human melanocytes to melanoma.  The biological information gained from this research has guided a second project that has produced a predictive model of the G2 checkpoint.