Core Staff

Staff contact information for the Rodent Advanced Surgical Models Core.

Phone numbers: (919) 962-4912              Fax number: (919) 966-6012

Location: 111 Mason Farm Road, MBRB, Suite 3341C Chapel Hill, NC 27599-7126

 Cooley picture

Core Faculty Director:  Brian C. Cooley, Ph.D.     


Journal Publications

Cooley BC, Nevado J, Mellad J, Yang D, St. Hilaire C, Negro A, Fang F, Chen G, San H, Walts AD, Schwartzbeck RL, Taylor B, Lanzer JD, Wragg A, Elagha A, Beltran LE, Berry C, Feil R, Virmani R, Ladich E, Kovacic JC, Boehm M:  TGF-β signaling mediates endothelial to mesenchymal transition (EndMT) during vein graft remodeling.  Science Translat Med 6:227ra34 [ePrint]   PMID:  24622514

Lee N, Daley RA, Cooley BC:  Rat posterior facial vein interpositional graft:  a more relevant training model.  Microsurgery 34:653–656, 2014.

 Cooley BC:  Collagen-induced thrombosis in murine arteries and veins.  Thrombos Res 131:49-54, 2013.

 Cooley BC, Chen CY, Hess R, Schmeling G:  Incomplete resolution of deep vein thrombosis under reduced flow conditions.  Thrombos Res  131:55-58, 2013.

 Zhi H, Rauova L, Hayes V, Gao C, Boylan B, Newman DK, McKenzie SE, Cooley BC, Poncz M, Newman PJ:  Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo.  Blood 121:1858-1867, 2013.  PMCID: PMC3591804

 McDonald RA, White KM, Wu J, Cooley BC, Robertson KE, Halliday C, Lu R, Kennedy S, George SJ, Wan S, van Rooij E, Baker AH:  MiRNA-21 is dysregulated in response to vein grafting in mulitple models and genetic ablation in mice attenuates neointima formation.  Eur Heart J 34:1636-1643, 2013 PMCID: PMC3675389

 Cooley BC, Herrera AJ:  Cross-modulatory effects of clopidogrel and heparin on platelet and fibrin incorporation in thrombosis.  Blood Coag Fibrinolysis 24:593-598, 2013

 Aleman MM, Walton BL, Byrnes JR, Wang JG, Heisler MJ, Machlus KR, Cooley BC, Wolberg AS:  Elevated prothrombin promotes venous, but not arterial, thrombosis in mice.  Arterioscler Thromb Vasc Biol 33:1829-1836, 2013

 Shi G, Meister D, Daley RA, Cooley BC:  Thrombodynamics of microvascular repairs: effects of antithrombotic therapy on platelets and fibrin.  J Hand Surg [Am] 38:1784-1789, 2013

 Cooley BC:  Murine models of thrombosis.  Thrombos Res 129 Suppl 2:S62-S64, 2012

 Maroney SA, Cooley BC, Ferrel JP, Bonesho CE, Nielsen LV, Johansen PB, Hermit MB, Petersen LC, Mast AE:  Absence of hematopoietic tissue factor pathway inhibitor mitigates bleeding in mice with hemophilia.  Proc Natl Acad Sci USA 109:3927-3931, 2012.  PMCID:  PMC3309760

 Khouri RK, Eisenmann-Klein M, Cardoso E, Cooley BC, Kacher D, Gombos E, Baker TJ:  Brava and autologous fat transfer is s  safe and effective breast augmentation alternative:  results of a six-year, eighty-one patients prospective multicenter study.  Plast Reconstr Surg 129:1173-1187, 2012.

 Yassai M, Cooley B, Gorski J:  Developmental dynamics of post selection thymic iNKT  PLoS ONE 7:e43509, 2012.  PMCID:  PMC3425480

 Smith AS, Choi SH, Collins JNR, Travers RJ, Cooley BC, Morrissey JH:  Inhibition of polyphosphate as a novel strategy for preventing thrombosis and inflammation.  Blood  120:5103-5110, 2012. PMCID: PMC3537307

 Kuether, EL, Fahs SA, Cooley BC, Schroeder JA, Montgomery RR, Wilcox DA, Shi Q:  Lentivirus-mediated platelet gene therapy of murine hemophilia A with pre-existing anti-FVIII immunity.  J Thromb Haemostas 10:1570-1580, 2012. PMCID: PMC3419807

 Maroney SA, Cooley BC, Ferrel JP, Bonesho CE, Mast AE:  Murine hematopoietic cell tissue factor pathway inhibitor limits thrombus growth.  Arterioscler Thromb Vasc Biol 31:821-826, 2011.  PMCID:  PMC3060281

 Cooley BC:  In vivo fluorescence imaging of large-vessel thrombosis in mice. Arterioscler Thromb Vasc Biol  31:1351-1356, 2011.  PMCID:  PMC3098306

 Ionova IA, Vásquez-Vivar J, Cooley BC, Khanna AK, Whitsett J, Herrnreiter A, Migrino RQ, Ge J-D, Regner KR, Channon KM, Alp NJ, Pieper GM:  Cardiac myocyte-specific over-expression of human GTP cyclohydrolase I protects against acute cardiac allograft rejection.  Am J Physiol - Heart Circ 299:H88-H96, 2010.  PMCID:  PMC2904123

 Pieper GM, Shah A, Harmann L, Cooley BC, Ionova IA, Migrino RQ:  Speckle-tracking 2-dimensional strain echocardiography:  A new noninvasive imaging tool to evaluate acute rejection in cardiac transplantation.  J Heart Lung Transplant 29:1039-1046, 2010.  PMCID:  PMC2926230

 Shi Q, Fahs SA, Kuether EL, Cooley BC, Weiler H, Montgomery RR:  Targeting FVIII expression to endothelial cells regenerates a releasable pool of FVIII and restores hemostasis in a mouse model of hemophilia A.  Blood 116:3049-3057, 2010.  PMCID:  PMC2974610

 Mosesson MW, Cooley BC, Hernandez I, Diorio JP, Weiler H:  Thrombosis risk modification in transgenic mice containing the human fibrinogen thrombin-binding gamma’ chain sequence.  J Thromb Haemost 7:102-110, 2009

 Rao R, Gourab K, Bagaria VB, Shidham VB, Metkar U, Cooley BC:  The effect of platelet rich plasma and bone marrow on murine posterolateral lumbar spine arthrodesis with bone morphogenetic protein.  J Bone Joint Surg [Am] 91:1199-1206, 2009.

 Pieper GM, Ionova IA, Cooley BC, Migrino RQ, Khanna AK, Whitsett J, Vásquez-Vivar J.  Sepiapterin decreases acute rejection and apoptosis in cardiac transplants independently of changes in nitric oxide and inducible nitric-oxide synthase dimerization.  J Pharmacol Exp Ther 320:890-899, 2009.  PMCID:  PMC2683770

 Maroney SA, Cooley BC, Sood R, Weiler H, Mast A:  Combined tissue factor pathway inhibitor and thrombomodulin deficiency produces an augmented hypercoagulable state with tissue specific fibrin deposition.  J Thromb Haemost 6:111-117, 2008.

 Rao R, Bagaria V, Gourab K, Haworth S, Shidham VB, Cooley BC:  Autograft containment in posterolateral spine fusion.  The Spine Journal 8:563-569, 2008.

 Ionova IA, Vásquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM:  Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.  Am J Physiol Heart Circ Physiol 295:H2178-H2187, 2008.  PMCID:  PMC2614582

 Rao R, Bagaria V, Cooley BC:  Posterolateral intertransverse lumbar fusion in a mouse model: surgical anatomy and operative technique. The Spine Journal 7:61-67, 2007.

 Cooley BC, Chen CY, Schmeling G:  Increased venous versus arterial thrombosis in the Factor V Leiden mouse.  Thrombosis Res 119:747-751, 2007

 Kusada A, Isogai N, Cooley BC:  Electric injury model of murine arterial thrombosis.  Thrombosis Res 121:103-106, 2007.

 Kerschen EJ, Fernandez JA, Cooley BC, Yang XV, Sood R, Mosnier LO, Castellino FJ, Mackman N, Griffin JH, Weiler H:  Endotoxemia and sepsis mortality reduction by non-anticoagulant activated protein C.  J Exp Med 204:2439-2448, 2007.  PMCID:  PMC2118455

 Cooley BC:  Differential neointimal response in vein grafts and wire-injured arteries. Circ J 71:1649-1652, 2007.

 Falati S, Patil S, Gross PL, Stapleton M, Merrill-Skoloff G, Barrett NE, Pixton KL, Weiler H, Cooley B, Newman DK, Newman PJ, Furie BC, Furie B, Gibbins JM:  Platelet PECAM-1 inhibits thrombus formation in vivo. Blood 107:535-541, 2006.  PMCID:  PMC1895610

 Shi Q, Wilcox DA, Fahs SA, Weiler H, Wells CW, Cooley BC, Desai D, Morateck PA, Gorski J, Montgomery RR:  Factor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high-titer inhibitory antibodies.  J Clin Invest 116:1974-1982, 2006.  PMCID:  PMC1483176

 Cooley BC, Chen C-Y, Friedman K, Datta YH:  A prothrombotic phenotype in the Copenhagen rat strain. Thrombosis Res 115:153-157, 2005.

 Cooley BC:  A model of murine interpositional vein grafting.  Microsurgery 25:209-212, 2005.

 Cooley BC, Daley RA, Toth JM:  Long-term BMP-2 induced bone formation in rat island and free flaps.  Microsurgery 25:167-173, 2005.

 Cooley BC, Szema L, Chen CY, Schwab JP, Schmeling G:  A murine model of deep vein thrombosis: characterization and validation in transgenic mice.  Thrombosis and Haemostasis 94:498-503, 2005.

 Cooley BC:  Murine model of neointimal formation and stenosis in vein grafts. Arterioscler Thromb Vasc Biol 24: 1180-1185, 2004.      

*Kerlin B, *Cooley BC, Isermann BH, Hernandez I, Sood R, Zogg M, Hendrickson SB, Mosesson MW, Lord S, Weiler H:  Cause-effect relation between hyperfibrinogenemia and vascular disease.  Blood 103:1728-1734, 2004.          [*Co-first-authors]

 Hsu PA, Cooley BC:  Effect of exercise on microsurgical hand tremor.  Microsurgery 23:323-327, 2003.

Datta YH, Wu FC, Dumas PC, Ning G, Datta MW, Cooley BC, Majewski R, Jensen-Seaman M, Jacob HJ:  Characterization and genetic mapping of the bleeding disorder in the fawn-hooded hypertensive rat. Thrombosis and Hemostasis 89:1031-1042, 2003.

 Khouri RK, Sherman R, Buncke HJ, Axel-Feller M, Hovius S, Benes CO, Ingram DM, Natarajan NN, Sherman JW, Yeramian PD, Cooley BC:  A Phase II trial of intraluminal irrigation with recombinant human tissue factor pathway inhibitor to prevent thrombosis in free flap surgery.  Plast Reconstr Surg 107:408-415, 2001.

 Cooley BC, Bergtrom G:  Multiple combinations of alternatively spliced exons in rat tropomyosin-a gene mRNA: evidence for 20 new isoforms in adult tissues and cultured cells.   Arch Biochem Biophys 390:71-77, 2001.

 Weiler H, Lindner V, Kerlin B, Isermann BH, Hendrickson SB, Cooley BC, Meh DA, Mosesson MW, Shworsk NW, Post MJ, Conway EM, Ulfman LH, von Andrian UH, Weitz JI:  Characterization of a mouse model for thrombomodulin deficiency.  Arterioscler Thromb Vasc Biol 21: 1531-1537, 2001.

 Tsai JC, Liu L, Cooley BC, Dichiara MR, Topper JN, Aird WC:  The Egr-1 promoter contains information for constitutive and inducible expression in transgenic mice.  FASEB J 14:1870-1872, 2000.

 Cooley BC, Daley RA:  Murine microvascular anastomosis model of thrombosis.   Thrombosis Res  96:157-159, 1999.

 Fowler JD, Li XL, Cooley BC:  Brief ex vivo perfusion with heparinized and/or citrated whole blood enhances tolerance of free muscle flaps to prolonged ischemia.  Microsurgery 19:135-140, 1999.

 Cooley BC:  The history of vein grafting.  Microsurgery 18:234-236, 1998.

 Khouri RK, Cooley BC, Kunselman AR, Landis JR, Yeramian P, Ingram D, Natarajan N, Benes CO, Wallemark C:  A prospective study of microvascular free flap surgery and outcome.  Plast Reconstr Surg 102:711-721, 1998.

 Cooley BC, Daley RA:  Free flap transplantation in mice.   Microsurgery 18:320-323, 1998.

 Lantieri LA, Ozbek MR, Deune EG, Ornberg RL, Brown DM, Chung SH, Wun T-C, Cooley BC, Khouri RK:  Prevention of microvascular thrombosis by topical application of tissue factor pathway  inhibitor.  Plast Reconstr Surg 97:587-594, 1996.

 Khouri RK, Koudsi B, Cooley BC, Deune EG, Gilula LA, Reddi AH:  Induction of normal bone by osteogenin, a bone morphogenetic protein:  Specificity of the responding tissue and requirement   for stem cell proliferation.  Plast Reconstr Surg 98:103-109, 1996.

 Isogai I, Cooley BC, Kamiishi H:  Clinical outcome of digital replantation using the fibrin glue-assisted microvascular anastomosis technique.  J Hand Surg (Brit & Europ Issue)21:573-575, 1996.

 Cooley BC:  Heat-induced tissue fusion in microvascular anastomosis.  Microsurgery 17:198-208, 1996.

 Hupkens P, Cooley BC:  Comparison of arterial and venous patency in a model of subendothelium-stimulated thrombosis.    Microsurgery 17:226-229, 1996.

 Jones R, Braam MJ, Cooley BC:  Arterial thrombosis enhanced by an acute phase response.  Annals Plast Surg 36:14-17, 1996.

 Li XL, Cooley BC, Fowler JD, Gould JS:  Intravascular heparin protects muscle flaps from ischemia/reperfusion injury.  Microsurgery 16:90-93, 1995.

 Braam MJ, Cooley BC, Gould JS:  Topical heparin enhances patency in a rat model of arterial thrombosis.  Annals Plast  Surg 34:148-153, 1995.

 Li XL, Cooley BC:  Effect of anticoagulation and inhibition of platelet aggregation on arterial versus venous microvascular thrombosis.  Annals Plast Surg 35:165-170, 1995.

 Ozbek MR, Brown DM, Deune EG, Lantieri LA, Kania NM, Pasia EN, Cooley BC, Wun T-C, Khouri RK:  Topical tissue factor pathway inhibitor improves free-flap survival in a model simulating free-flap errors.  J Reconstr Microsurg 11:185-188, 1995.

 Cooley BC, Gould JS:  Investigation in the use of topical solutions for management or prevention of thrombosis in traumatized vessels.  Internatl Angiology 14:297-302, 1995.

 Ozbek M, Deune EG, Cooley BC, Khouri RK:  Experimental reproduction of free flap errors:  A new model of thrombosis.   Annals Plast Surg 32:474-477, 1994.                             

Cooley BC:  Laser-assisted microvascular anastomoses:  In vitro and in vivo evaluation.  Revista de Orthoped y Traumatol (Spain) 38 IB: 310-319, 1994.

 Cooley BC, Gould JS:  The influence of age upon rat free flap survival following prolonged cold ischemia.  Annals Plast  Surg 30:57-59, 1993.

 Li XL, Cooley BC, Gould JS:  Ex vivo perfusion with anticoagulated blood decreases ischemia/reperfusion injury.  J Hand Surg 18A:629-634, 1993

Li XL, Cooley BC, Gould JS:  Effect of age upon ischemia-induced muscle injury in a rat muscle free flap model.  J Surg Research 55:193-200, 1993.

 Kulwicki KJ, Harris GF, Cooley BC:  A system for evaluation of surgical hand tremor.  Proc Ann Internat Conf IEEE Eng Med Biol Soc 15:977-978, 1993.

 Cooley BC, Hanel DP, Ma L, Li XL, Gould JS:  The influence of diabetes upon free tissue transfer:  Part II.  The effect of ischemia on flap viability.  Annals Plast Surg 29:65-69, 1992.

 Li XL, Cooley BC, Gruel SM, Ye Z, Gould JS:  The rat cutaneous maximus free muscle flap model:  Comparison to the latissimus dorsi flap.   Microsurgery 13:208-213, 1992.

 Cooley BC, Li XL, Dzwierzynski W, Gruel SM, Wright RR, Hall R, O'Brien EM, Fagan D, Hanel DP, Gould JS:  The de-endothelialized rat carotid arterial graft:  A versatile experimental model for the investigation of arterial thrombosis.  Thrombosis Research 67:1-14, 1992              

 Cooley BC, Fowler JD, Gould JS:  Influence of physiologic alteration of fibrinogen levels upon micro-venous anastomotic patency.  Microsurgery 13:299-303, 1992.    

Cooley BC, Dollinger BM, Mlsna JS, Gould JS:  Maintenance of patency after vascular trauma by topical irrigation with a peptide homologous to the carboxy-terminus of the fibrinogen gamma-chain.  J Hand Surg 17A:1044-1051, 1992.

 Cooley BC, Hanel DP, Gould JS, Li XL, Smith JW:  Antithrombotic benefit of subendothelium-bound urokinase:  An experimental study.  J Hand Surg 17A:235-244, 1992.

 Rumbolo PM, Cooley BC, Hanel DP, Gould JS:  Comparison of the influence of intralumenal irrigation solutions on free flap survival.  Microsurgery 13:45-47, 1992.               

Li XL, Cooley BC, Gould JS:  The influence of topical heparin on stasis-induced thrombosis of microvascular anastomoses.   Microsurgery 13:72-75, 1992.

 Ma L, Li XL, Cooley BC, Gould JS:  Microvascular salvage procedures with adjuvant antithrombotic therapy for restitution of patency in a rat model of vein thrombosis.  J Reconstr  Microsurg 8:201-205, 1992.

  Cooley BC, Gould JS:  Investigation of the effect of venous flow alterations upon patency of rat femoral vein anastomoses.      Microsurgery 13:138-142, 1992.

 Cooley BC, Hanel DP, Anderson RB, Foster MD, Gould JS:   The influence of diabetes upon free tissue transfer:  Part I.  Flap survival and anastomotic healing.  Annals Plast Surg 29:58-64, 1992.

 Cooley BC:  Rat models for investigating clinical problems in free flap surgery.  Revista de Orthoped y Traumatol (Spain) 35 IB:406-415, 1991.

 Cooley BC:  Standardization of microsurgery training.  Revista de Orthoped y Traumatol (Spain) 35 IB:401-405, 1991.

 Savoie FH, Cooley BC, Gould JS:  Evaluation of pharmacological agents on crush-avulsion arterial injuries: a scanning electron microscopy study.  Microsurgery 12:292-300, 1991.

 Cooley BC, Ma L, Gould JS:  Rat femoral vein-to-vein grafts as a microvascular practice model:  Factors that influence patency.  Microsurgery 12:43-45, 1991.

 Cooley BC, Ma L, Groner JP, Hoer SR, Dollinger BM, Hanel DP: An experimental study of the influence  of arterial trauma upon dependent distal tissue survival.  Microsurgery 12:86-88, 1991.

 Cooley BC, Gould JS:  Topically applied antithrombotic agents offer a new therapeutic approach to the prevention of microvascular thrombosis.  Microsurgery 12:281-287, 1991.

 Cooley BC, Tadych KL, Gould JS:  Reperfusion of free flaps during normothermic ischemic storage.   J  Reconstr  Microsurg      6:49-53, 1990.

 Khouri RK, Cooley BC, Kenna DM, Edstrom L:  Thrombosis of microvascular anastomoses in traumatized vessels:  Fibrin  versus platelets.  Plastic Reconstr Surg 86:110-117, 1990.

 Stein BS, Cooley BC:  CO2 laser-assisted microscopic vasovasostomy.  J Endourology 2:299-307, 1988.

 Wright J, Cooley BC, Duwell J, Sieber-Blum M:  Migration-related changes in the cytoskeleton of cultured neural crest cells visualized by the monoclonal antibody I-5G9.  J Neurosci Res 21:148-154, 1988.

 Cooley BC, Ruas EJ, Wilgis EFS:  Scanning electron microscopy of crush/avulsion arterial trauma:  Effect of heparin and aspirin administration.  Microsurgery 8:11-16, 1987.

 Cooley BC, Gould JS:  Experimental models for evaluating antithrombotic therapies in  replantation  microsurgery.    Microsurgery 8:230-233, 1987.

 Schlaff WD, Cooley BC, Shen W, Rock J, King T:  A rat uterine horn model of genital tract wound healing.  Fertility Sterility 48:866-872, 1987.

 Jarow JP, Cooley BC, Marshall FF:  Laser-assisted vasal anastomosis in the rat and man.  J Urology 36:1132-1135, 1986.

 Cooley BC, Hansen FC:  Microvascular repair following local crush and avulsion vascular injury.  Microsurgery 6:46-48, 1985.

 Cooley BC, Morgan RF, Dellon AL:  Thrombolytic reversal of no-reflow phenomenon in rat free flap model.  Surgical Forum 34:638-640, 1983.                                            

Cooley BC, Jones MM, Dellon AL:  Comparison  of  efficacy  of Thrombolysin, streptokinase, and urokinase in a femoral vein clot model in rats.  Microsurgery 4:1-4, 1983.

 Cooley BC, Hansen FC, Dellon AL:  The effect of temperature on ischemic tolerance of experimental free flaps.  J Microsurgery 3:11-14, 1981.

 Gould E, Cooley BC, Barnick P:  Echolocation by young bats on their initial and subsequent flights.  Developmental Psychobiol 14:41-54, 1981.

 Dellon AL, Curtis RM, Cooley BC:  Lysis of venous clots by local intra-arterial Thrombolysin injection in the rat.  Annals Plast Surg 7:38-43, 1981.

 Swartzwelder HS, Johnson CT, Cooley BC, Howell WE, Dyer RS:  Alcohol-induced alterations in hippocampal afterdischarges and afterdischarge thresholds:  Dose response studies.  Neurobehav  Toxicol 1:253-258, 1980.