Peer-reviewed Publications

Cardiovascular Model-Relevant Publications:

 Gherghe CM, Duan J, Gong J, Rojas M, Klauber-Demore N, Majesky M, Deb A. Wnt1 is a proangiogenic molecule, enhances human endothelial progenitor function, and increases blood flow to ischemic limbs in a HGF- dependent manner. FASEB J. 2011 Feb 14

 Niu XL, Madamanchi NR, Vendrov AE, Tchivilev I, Rojas M, Madamanchi C, Brandes RP, Krause KH, Humphries J, Smith A, Burnand KG, Range MS. Nox activator 1: a potential target for modulation of vascular reactive oxygen species in atherosclerotic arteries. Circulation. 2010 Feb 2; 121(4): 549-59. Epub 2010, Jan 18.

 Antoniak S, Rojas M, Spring D, Bullard T, Verrier E, Bacall B, Mackman N, Pawlinski R. Protease-Activated Receptor 2 Deficiency Reduces Cardiac Ischemia/Reperfusion Injury. Arterioscler Thrombi Vass Biol. 2010, Aug 19.

 Jerath MR, Liu P, Struthers M, Demetrio JA, Peng R, Peterson LB, Cumiskey AM, Yang L, Rojas M, Patel DD, Fong AM. Dual targeting of CCR2 and CX3CR1 in an arterial injury model of vascular inflammation. Thrombi J. 2010 Sep 13;8(1): 14

 Hakim ZS, DiMichele LA, Rojas M, Mack CP, Taylor JM. FAK regulates cardiomyocyte survival following ischemia/reperfusion. J Mol Cell Cardiol. 2009 Feb; 46(2): 241-8.

 Dimichele LA, Hakim ZS, Sayers RL, Rojas M, Schwartz RJ, Mack CP, Taylor JM. Transient expression of FRNK reveals stage-specific requirement for focal adhesion kinase activity in cardiac growth. Circ Res. 2009, May 22; 104 (10): 1201-8

 Barrack CJ, Roberts RB, Rojas M, Rajamannan NM, Suitt CB, O'Brien KD, Smyth SS, Threadgill DW. Reduced EGFR causes abnormal valvular differentiation leading to calcific aortic stenosis and left ventricular hypertrophy in C57BL/6J but not 129S1/SvImJ mice. Am J Physiol Heart Circ Physiol. 2009 Jul; 297(1): 65-75

Willis MS, Rojas M, Li L, Selzman CH, Tang RH, Stansfield WE, Rodriguez JE, Glass DJ, Patterson C.Muscle ring finger 1 mediates cardiac atrophy in vivo. Am J Physiol Heart Circ Physiol. 2009 Apr; 296(4): H997-H1006.

 Stansfield WE, Charles PC, Tang RH, Rojas M, Bhati R, Moss NC, Patterson C, Selzman CH. Regression of pressure-induced left ventricular hypertrophy is characterized by a distinct gene expression profile. J Thorac Cardiovasc Surg. 2009 Jan; 137(1): 232-8, 238e1-8

 Chen J, Murchison E, Tang R, Callis T, Tatsuguchi M, Deng Z, Rojas M, Hammond S, Schneider M, Selzman CH, Meissner G, Patterson C, Hannon G, Wang DZ. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci USA. 2008; 105: 2111-6.

 Pandya K, Cowhig J, Brackhan J, Kim HS, Hagaman J, Rojas M, Carter Jr, Mao L, Rockman HA, Maeda N, Smithies O. Discordant on/off switching of gene expression in myocytes during cardiac hypertrophy in vivo.Proc Natl Acad Sci U S A. 2008 Aug 28.

 Sayers RL, Sundberg-Smith LJ, Rojas M, Hayasaka H, Parsons JT, Mack CP, Taylor RL. FRNK expression promotes smooth muscle cell maturation during vascular development and after vascular injury. Arterioscler Throm Vasc Bio. 2008 Sep 11, 18787183

 Vendrov AE, Madamanchi N, Hakim ZS, Rojas M, Runge MS. Thrombin and NAD (P) H Oxidase-Mediated Regulation of CD44 and BMP4-Id Pathway in VSMC, Restenosis, and Artherosclerosis. Circulation Research. 2006; 98:1254-1263.

 DiMichele LD, Doherty JT, Rojas M, Mack CP, Beggs HE, Reichardt LF and Taylor JM. Cardiac-restricted FAK inactivation attenuates pressure-overload 
induced hypertrophy. Circulation Research. 2006

 Liu P, Patil S., Rojas M, Fong A., Smyth S.S., and Patel D.D. CX3CR1 deficiency confers protection from intimal hyperplasia after arterial injury. ATVB. 2006; 26:2056-2062

 Evangelista V, Pamuklar Z, Piccoli A, Manarini S, Dell'Elba G, Pecce R, Martelli N, Federico L, Rojas M, Berton G, Lowell CA, Totani L, and Smyth SS. Src family kinases mediate neutrophil adhesion to adherent platelets. Blood. 2007 Mar 15;109(6):2461-9.

 Barrick C, Rojas M, Schoonhoven R, Smyth S, Threadgill DW. Cardiac response to pressure overload in 129S1/SvImJ and C57BL/6J mice: temporal and background dependent development of concentric left ventricular hypertrophy. Am J Physiology Heart Circ Physiol. 2006 

Tatsuguchi M, Seok HY, Callis TE, Thomson JM, Chen JF, Newman M, Rojas M, Hammond SM, Wang DZ. Expression of microRNAs is dynamically regulated during cardiomyocyte hypertrophy. J Mol Cell Cardiol. 2007 Apr14.

 Niu XL, Li J, Hakim ZS, Rojas M, Runge MS, Madamanchi NR. Lar deficiency enhances IGF-1 signaling in vascular smooth muscle cells and promotes neointima formation in response to vascular injury.  J Biol Chem. 2007 May 11

 Stansfield WE, Rojas M, Corn D, Willis M, Patterson C, Smyth SS, Selzman CH. Characterization of a Model to Independently Study Regression of Ventricular Hypertrophy. J Surg Res. 2007 Oct; 142(2): 387-93.

 Vendrov AE, Hakim ZS, Madamanchi NR, Rojas M, Madamanchi C, and Runge MS. Atherosclerosis Is Attenuated by Limiting Superoxide Generation in Both Macrophages and Vessel Wall Cells. Arterioscler. Thromb. Vasc. Biol. 2007 Dec; 27(12): 2714-21.


Mouse/Rat Heart Transplantation

 Ionova IA, Vásquez-Vivar J, Cooley BC, Khanna AK, Whitsett J, Herrnreiter A, Migrino RQ, Ge J-D, Regner KR, Channon KM, Alp NJ, Pieper GM:  Cardiac myocyte-specific over-expression of human GTP cyclohydrolase I protects against acute cardiac allograft rejection.  Am J Physiol - Heart Circ 299:H88-H96, 2010.  PMCID:  PMC2904123

 Pieper GM, Ionova IA, Cooley BC, Migrino RQ, Khanna AK, Whitsett J, Vásquez-Vivar J.  Sepiapterin decreases acute rejection and apoptosis in cardiac transplants independently of changes in nitric oxide and inducible nitric-oxide synthase dimerization.   J Pharmacol Exp Ther 329:890-899, 2009.  PMCID:  PMC2683770

 Pieper GM, Shah A, Harmann L, Cooley BC, Ionova IA, Migrino RQ:  Speckle-tracking 2-dimensional strain echocardiography:  A new noninvasive imaging tool to evaluate acute rejection in cardiac transplantation.  J Heart Lung Transplant 29:1039-1046, 2010.   PMCID:  PMC2926230

 Ionova IA, Vásquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM:  Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.  Am J Physiol Heart Circ Physiol 295:H2178-H2187, 2008.  PMCID:  PMC2614582


 Mouse/Rat Free Flap Transfer/Transplantation

 Cooley BC, Daley RA:  Free flap transplantation in mice.   Microsurgery 18:320-323, 1998.

 Cooley BC, Daley RA, Toth JM:  Long-term BMP-2 induced bone formation in rat island and free flaps.  Microsurgery 25:167-173, 2005.

 Khouri RK, Sherman R, Buncke HJ, Axel-Feller M, Hovius S, Benes CO, Ingram DM, Natarajan NN, Sherman JW, Yeramian PD, Cooley BC:  A Phase II trial of intraluminal irrigation with recombinant human tissue factor pathway inhibitor to prevent thrombosis in free flap surgery.  Plast Reconstr Surg 107:408-415, 2001.

 Fowler JD, Li XL, Cooley BC:  Brief ex vivo perfusion with heparinized and/or citrated whole blood enhances tolerance of free muscle flaps to prolonged ischemia.  Microsurgery 19:135-140, 1999.

 Khouri RK, Cooley BC, Kunselman AR, Landis JR, Yeramian P, Ingram D, Natarajan N, Benes CO, Wallemark C:  A prospective study of microvascular free flap surgery and outcome.  Plast Reconstr Surg 102:711-721, 1998.

 Cooley BC, Hansen FC, Dellon AL:  The effect of temperature on ischemic tolerance of experimental free flaps.  J Microsurgery 3:11-14, 1981.

 Cooley BC, Morgan RF, Dellon AL:  Thrombolytic reversal of no-reflow phenomenon in rat free flap model.  Surgical Forum 34:638-640, 1983.

Cooley BC, Tadych KL, Gould JS:  Reperfusion of free flaps during normothermic ischemic storage.   J  Reconstr  Microsurg      6:49-53, 1990.

Cooley BC, Hanel DP, Anderson RB, Foster MD, Gould JS:   The influence of diabetes upon free tissue transfer:  Part I.  Flap survival and anastomotic healing.  Annals Plast Surg 29:58-64, 1992.

Cooley BC, Hanel DP, Ma L, Li XL, Gould JS:  The influence of diabetes upon free tissue transfer:  Part II.  The effect of ischemia on flap viability.  Annals Plast Surg 29:65-69, 1992.

Li XL, Cooley BC, Gruel SM, Ye Z, Gould JS:  The rat cutaneous maximus free muscle flap model:  Comparison to the latissimus dorsi flap.   Microsurgery 13:208-213, 1992.

Cooley BC, Gould JS:  The influence of age upon rat free flap survival following prolonged cold ischemia.  Annals Plast  Surg 30:57-59, 1993.

 Li XL, Cooley BC, Gould JS:  Ex vivo perfusion with anticoagulated blood decreases ischemia/reperfusion injury.  J Hand Surg 18A:629-634, 1993.

 Li XL, Cooley BC, Gould JS:  Effect of age upon ischemia-induced muscle injury in a rat muscle free flap model.  J Surg Research 55:193-200, 1993.

Li XL, Cooley BC, Fowler JD, Gould JS:  Intravascular heparin protects muscle flaps from ischemia/reperfusion injury.  Microsurgery 16:90-93, 1995.

Ozbek MR, Brown DM, Deune EG, Lantieri LA, Kania NM, Pasia EN, Cooley BC, Wun T-C, Khouri RK:  Topical tissue factor pathway inhibitor improves free-flap survival in a model simulating free-flap errors.  J Reconstr Microsurg 11:185-188, 1995.


Mouse/Rat Vein Graft Models and Applications

 Cooley BC:  Murine model of neointimal formation and stenosis in vein grafts. Arterioscler Thromb Vasc Biol 24: 1180-1185, 2004.

 Cooley BC, Nevado J, Mellad J, Yang D, St. Hilaire C, Negro A, Fang F, Chen G, San H, Walts AD, Schwartzbeck RL, Taylor B, Lanzer JD, Wragg A, Elagha A, Beltran LE, Berry C, Feil R, Virmani R, Ladich E, Kovacic JC, Boehm M:  TGF-β signaling mediates endothelial to mesenchymal transition (EndMT) during vein graft remodeling.  Science Translat Med 6:227ra34 [ePrint]   PMID:  24622514

 Lee N, Daley RA, Cooley BC:  Rat posterior facial vein interpositional graft:  a more relevant training model.  Microsurgery 34:653–656, 2014.

 McDonald RA, White KM, Wu J, Cooley BC, Robertson KE, Halliday C, Lu R, Kennedy S, George SJ, Wan S, van Rooij E, Baker AH:  MiRNA-21 is dysregulated in response to vein grafting in mulitple models and genetic ablation in mice attenuates neointima formation.  Eur Heart J 34:1636-1643, 2013 PMCID: PMC3675389

 Cooley BC:  Differential neointimal response in vein grafts and wire-injured arteries. Circ J 71:1649-1652, 2007.

 Cooley BC:  A model of murine interpositional vein grafting.  Microsurgery 25:209-212, 2005.


 Other Organ Manipulations

 Tsai JC, Liu L, Cooley BC, Dichiara MR, Topper JN, Aird WC:  The Egr-1 promoter contains information for constitutive and inducible expression in transgenic mice.  FASEB J 14:1870-1872, 2000.

 Yassai M, Cooley B, Gorski J:  Developmental dynamics of post selection thymic iNKT
PLoS ONE 7:e43509, 2012.  PMCID:  PMC3425480

 Schlaff WD, Cooley BC, Shen W, Rock J, King T:  A rat uterine horn model of genital tract wound healing.  Fertility Sterility 48:866-872, 1987.


Mouse Thrombosis/Hemostasis Studies

 Cooley BC:  In vivo fluorescence imaging of large-vessel thrombosis in mice. Arterioscler Thromb Vasc Biol  31:1351-1356, 2011.  PMCID:  PMC3098306

Cooley BC:  Murine models of thrombosis.  Thrombos Res 129 Suppl 2:S62-S64, 2012.

 Cooley BC:  Collagen-induced thrombosis in murine arteries and veins.  Thrombos Res 131:49-54, 2013.

 Cooley BC, Chen CY, Hess R, Schmeling G:  Incomplete resolution of deep vein thrombosis under reduced flow conditions.  Thrombos Res  131:55-58, 2013.

 Zhi H, Rauova L, Hayes V, Gao C, Boylan B, Newman DK, McKenzie SE, Cooley BC, Poncz M, Newman PJ:  Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo.  Blood 121:1858-1867, 2013.  PMCID: PMC3591804

 Cooley BC, Herrera AJ:  Cross-modulatory effects of clopidogrel and heparin on platelet and fibrin incorporation in thrombosis.  Blood Coag Fibrinolysis 24:593-598, 2013

 Aleman MM, Walton BL, Byrnes JR, Wang JG, Heisler MJ, Machlus KR, Cooley BC, Wolberg AS:  Elevated prothrombin promotes venous, but not arterial, thrombosis in mice.  Arterioscler Thromb Vasc Biol 33:1829-1836, 2013

 Shi G, Meister D, Daley RA, Cooley BC:  Thrombodynamics of microvascular repairs: effects of antithrombotic therapy on platelets and fibrin.  J Hand Surg [Am] 38:1784-1789, 2013

 Maroney SA, Cooley BC, Ferrel JP, Bonesho CE, Nielsen LV, Johansen PB, Hermit MB, Petersen LC, Mast AE:  Absence of hematopoietic tissue factor pathway inhibitor mitigates bleeding in mice with hemophilia.  Proc Natl Acad Sci USA 109:3927-3931, 2012.   PMCID:  PMC3309760

 Smith AS, Choi SH, Collins JNR, Travers RJ, Cooley BC, Morrissey JH:  Inhibition of polyphosphate as a novel strategy for preventing thrombosis and inflammation.  Blood  120:5103-5110, 2012. PMCID: PMC3537307

 Kuether, EL, Fahs SA, Cooley BC, Schroeder JA, Montgomery RR, Wilcox DA, Shi Q:  Lentivirus-mediated platelet gene therapy of murine hemophilia A with pre-existing anti-FVIII immunity.  J Thromb Haemostas 10:1570-1580, 2012. PMCID: PMC3419807

 Maroney SA, Cooley BC, Ferrel JP, Bonesho CE, Mast AE:  Murine hematopoietic cell tissue factor pathway inhibitor limits thrombus growth.  Arterioscler Thromb Vasc Biol 31:821-826, 2011.  PMCID:  PMC3060281

 Shi Q, Fahs SA, Kuether EL, Cooley BC, Weiler H, Montgomery RR:  Targeting FVIII expression to endothelial cells regenerates a releasable pool of FVIII and restores hemostasis in a mouse model of hemophilia A.  Blood 116:3049-3057, 2010.  PMCID:  PMC2974610

 Mosesson MW, Cooley BC, Hernandez I, Diorio JP, Weiler H:  Thrombosis risk modification in transgenic mice containing the human fibrinogen thrombin-binding gamma’ chain sequence.  J Thromb Haemost 7:102-110, 2009

 Maroney SA, Cooley BC, Sood R, Weiler H, Mast A:  Combined tissue factor pathway inhibitor and thrombomodulin deficiency produces an augmented hypercoagulable state with tissue specific fibrin deposition.  J Thromb Haemost 6:111-117, 2008.

 Cooley BC, Chen CY, Schmeling G:  Increased venous versus arterial thrombosis in the Factor V Leiden mouse.  Thrombosis Res 119:747-751, 2007

 Kusada A, Isogai N, Cooley BC:  Electric injury model of murine arterial thrombosis.  Thrombosis Res 121:103-106, 2007.

 Falati S, Patil S, Gross PL, Stapleton M, Merrill-Skoloff G, Barrett NE, Pixton KL, Weiler H, Cooley B, Newman DK, Newman PJ, Furie BC, Furie B, Gibbins JM:  Platelet PECAM-1 inhibits thrombus formation in vivo. Blood 107:535-541, 2006.  PMCID:  PMC1895610

 Shi Q, Wilcox DA, Fahs SA, Weiler H, Wells CW, Cooley BC, Desai D, Morateck PA, Gorski J, Montgomery RR:  Factor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high-titer inhibitory antibodies.  J Clin Invest 116:1974-1982, 2006.  PMCID:  PMC1483176

 Cooley BC, Szema L, Chen CY, Schwab JP, Schmeling G:  A murine model of deep vein thrombosis: characterization and validation in transgenic mice.  Thrombosis and Haemostasis 94:498-503, 2005.

  *Kerlin B, *Cooley BC, Isermann BH, Hernandez I, Sood R, Zogg M, Hendrickson SB, Mosesson MW, Lord S, Weiler H:  Cause-effect relation between hyperfibrinogenemia and vascular disease.  Blood 103:1728-1734, 2004.          [*Co-first-authors]

 Weiler H, Lindner V, Kerlin B, Isermann BH, Hendrickson SB, Cooley BC, Meh DA, Mosesson MW, Shworsk NW, Post MJ, Conway EM, Ulfman LH, von Andrian UH, Weitz JI:  Characterization of a mouse model for thrombomodulin deficiency.  Arterioscler Thromb Vasc Biol 21: 1531-1537, 2001.

 Cooley BC, Daley RA:  Murine microvascular anastomosis model of thrombosis.   Thrombosis Res  96:157-159, 1999.


 Murine Sepsis Models

 Kerschen EJ, Fernandez JA, Cooley BC, Yang XV, Sood R, Mosnier LO, Castellino FJ, Mackman N, Griffin JH, Weiler H:  Endotoxemia and sepsis mortality reduction by non-anticoagulant activated protein C.  J Exp Med 204:2439-2448, 2007.  PMCID:  PMC2118455


Laser Tissue Welding

 Cooley BC:  Heat-induced tissue fusion in microvascular anastomosis.  Microsurgery 17:198-208, 1996.

 Jarow JP, Cooley BC, Marshall FF:  Laser-assisted vasal anastomosis in the rat and man.  J Urology 36:1132-1135, 1986.

 Stein BS, Cooley BC:  CO2 laser-assisted microscopic vasovasostomy.  J Endourology 2:299-307, 1988.


 Spine Fusion Models

 Rao R, Gourab K, Bagaria VB, Shidham VB, Metkar U, Cooley BC:  The effect of platelet rich plasma and bone marrow on murine posterolateral lumbar spine arthrodesis with bone morphogenetic protein.  J Bone Joint Surg [Am] 91:1199-1206, 2009.

 Rao R, Bagaria V, Gourab K, Haworth S, Shidham VB, Cooley BC:  Autograft containment in posterolateral spine fusion.  The Spine Journal 8:563-569, 2008.

 Rao R, Bagaria V, Cooley BC:  Posterolateral intertransverse lumbar fusion in a mouse model: surgical anatomy and operative technique. The Spine Journal 7:61-67, 2007.