Rodent Cardiovascular Models
- LAD Ischemia/Reperfusion: Ligation of the left anterior descending artery of the heart with release and reperfusion post-ischemia – both acute and survival surgery procedures offered, as models of myocardial infarction.
- Renal Artery Stenosis (with laser Doppler flow measurement): Surgical narrowing of the renal artery, as a model of systemic hypertension.
- Aortic Banding: Surgical narrowing of the aortic arch to induce left ventricular pressure-volume overload; reversible as a model of hypertrophic regression.
- Renal Artery Stenosis: surgical narrowing of the renal artery as a model of hypertension.
- Pulmonary Artery Banding: used as a model of pulmonary hypertension.
- Echocardiography: Ultrasound imaging of the heart in 2-D or 3-D.
- Ventricular Pressure-Volume Measurement: Direct measurement of cardiac left ventricle blood pressure and stroke volume (Scisense).
- Vascular catheterization: insertion of a catheter into an artery or vein, for infusion, blood withdrawal, or blood pressure measurements.
- Invasive Blood Pressure Measurement: Direct measurement of intra-arterial pressure(s) via Scisense catheter insertion or telemetry device implantation.
- Tail-Cuff Blood Pressure Measurement: non-invasive BP measurement.
- Femoral/Carotid Artery (Wire) Injury: Removal of endothelium and subendothelial injury to induce neointimal formation.
- Carotid Artery Ligation: ligation of one carotid artery to induce neointimal remodeling.
- Hindlimb Ischemia (with laser Doppler flow measurement): Ligation of the hindlimb artery to induce angiogenesis/collateralization.
- Interpositional vascular grafting: Placement of a segment of vein, artery, aortic arch, or synthetic/bioengineered graft into the arterial or venous circulation (typically the aorta, carotid, or femoral artery in rodents), either in end-to-end or end-to-side anastomotic configurations, including arteriovenous shunt creation (fistula).
- Miscellansous Ultrasound Evaluations: measurements made by ultrasound of organs (e.g., echocardiography of the heart), in 2D or 3D, or for ultrasound-guided injection.
- Tissue/organ harvests: dissection of structures on the tissue (e,g, whole aortic tree) or whole-organ level, including using whole-body perfusion-fixation.
- Mini-Pump Implantation: for continuous subcutaneous or intravenous infusion of substances.
A variety of rodent heterotopic and orthotopic transplantation models are available, for which the Core lab has expertise for successful accomplishment. These can be done as syngenic transplants (inbred, with no immunorejection) or allotransplants (with various strain-based immunologic barriers).
- Heterotopic Heart Transplantation
- Renal Transplantation
- Liver Transplantation
- Pancreatic Transplantation
- Small Bowel Transplantation
- Esophageal or Tracheal Transplantation
- Uterine Horn Transplantation
- Aortic (Vascular) Graft Transplantation
- Free Composite Tissue Vascularized Transplantation (both standard and custom-designed models)
Thrombosis and Hemostasis
- Large-Vessel Electrolytic Injury (with intravital fluorescence imaging and quantitative data analysis)
- Large-Vessel Collagen Induction (with intravital fluorescence imaging and quantitative data analysis)
- Large-Vessel Mechanical Injury (with intravital fluorescence imaging and quantitative data analysis)
- Microvessel Laser Injury (with intravital fluorescence imaging and quantitative data analysis); typically the cremasteric or mesenteric microvascular prep
- Microvessel Ferric Chloride Injury; cremasteric or mesenteric microvascular prep
- Carotid Artery Ferric Chloride Injury
- Inferior Vena Cava Ligation (either total or partial ligation)
- Saphenous Vein Laser Puncture Haemostasis Assay
- Saphenous Vein Stab Puncture Haemostasis Assay
- Tail Bleeding Assay
- Tail/Microvessel Bleeding (with intravital fluorescence imaging)
Intravital fluorescence imaging is done standardly with two simultaneous fluorophores, one to document platelet development and the other as a fibrin label (anti-fibrin linked to a fluorophore). Data are quantitated and normalized for each experimental run, for inter-animal comparisons. Custom-labeled antibodies/molecules/cells can also be used with this imaging platform.
Cooley BC: In vivo fluorescence imaging of large-vessel thrombosis in mice. Arterioscler Thromb Vasc Biol 31:1351-1356, 2011.
Custom-Designed Fluorescence Imaging Applications
The intravital fluorescence imaging system in the Advanced Surgery Core can be modified for a variety of purposes, to adapt to specific scientific applications as needed. Some of the features include:
- Up to 3 simultaneous fluorophores, using many different wavelengths for excitation/emission, from violet to near-infrared
- Up to 100X magnification, at up to a resolution of 2X2 microns/pixel
- Time-lapse video acquisition with subsequent video-clip development for presentations and publications
- Quantitative capabilities (via video image analysis)
Several other surgical procedures are offered, through the expertise of the staff. In addition, the Core staff is willing to work with PIs and the IACUC to develop new models for research-specific applications:
- Intra-thymic injection
- Spine fusion surgery
- Organ excision (splenectomy, nephrectomy, orchiestomy, ovariectomy, adrenalectomy)
- Uterine horn anastomosis
- Cecal puncture sepsis model
- Other procedures by request and co-development]