11-141 Lineberger Comprehensive Cancer Center
Dr. Nelson is Director of the CFAR Virology Core Laboratory and supervises Molecular Assay Development. The Core provides expertise in HIV molecular assays, develops sequencing protocols as needed by other researchers, and tests compounds for anti-HIV activity. Dr. Nelson is also Associate Director of the Retrovirology Laboratory that performs virologic assays for adult and pediatric clinical trials in the ACTG, IMPAACT, and HPTN networks, as well as investigator-initiated clinical trials at UNC.
The focus of research in the laboratory has been trying to understand the factors associated with the transmission of HIV and from there, targeting ways to prevent transmission. We have developed methods of quantitating HIV in blood, genital secretions, breast milk, CSF, urine, and throatwash in support of multiple studies. These methods are used in studies of compartmentalization of HIV in the body, and to assess the effectiveness of antiretroviral treatment throughout these compartments. In addtion, we are evaluating new methods for point of care diagnosis of infants and virologic monitoring during antiretroviral treatment, and helping to develop a low-tech, inexpensive method of infant diagnosis.
Farr S.L., J. A. E. Nelson, T. J. Ng’ombe, A.P. Kourtis, C. Chasela, J. A. Johnson, A. D. M. Kashuba, G. L. Tegha, J. Wiener, J. J. Eron, H. N. Banda, M. Mpaso, J. Lipscomb, C. Matiki, S. A. Fiscus, D. J. Jamieson, and C. van der Horst. 2010. Addition of 7 Days of Zidovudine plus Lamivudine to Peripartum Single-Dose Nevirapine Effectively Reduces Nevirapine Resistance Postpartum in HIV-Infected Mothers in Malawi. JAIDS, 54:515-523.
Hurt C. B., S. I. McCoy, J. Kuruc, J. Nelson, M. Kerkau, S. Fiscus, K. McGee, J. Sebastian, P. Leone, C. Pilcher, C. Hicks, and J. Eron, Jr. 2009. Transmitted Antiretroviral Drug Resistance among Acute and Recent HIV Infections in North Carolina, 1998 to 2007. Antivir. Ther. 14:673-678.
Hosseinipour M. C., J. J. G. van Oosterhout, R. Weigel, S. Phiri, D. Kamwendo, N. Parkin, S.A. Fiscus, J. A. E. Nelson, J. J. Eron, J. Kumwenda. 2009. The public health approach to identify antiretroviral (ART) failure: high level NRTI resistance among Malawians failing first line ART. AIDS 23:1127-1134.
Nelson J. A. E., A. M. Loftis, D. Kamwendo, W. W. Fawzi, T. E. Taha, R. L. Goldenberg, S. A, Fiscus. 2009. Nevirapine resistance in human immunodeficiency virus type 1-positive infants determined using dried blood spots stored for up to six years at room temperature. J Clin Microbiol. 47:1209-1211.
Kerr, R. J. S., G. Player, S. A. Fiscus, and J. A. E. Nelson. 2009. Qualitative HIV RNA analysis of dried blood spots for the diagnosis of infants. J Clin Microbiol, 47:220-2.
Riddle, T. M., N. J. Shire, M. S. Sherman, K. F. Franco, H. W. Sheppard, and J. A. E. Nelson. 2006. Sequential turnover of human immunodeficiency virus type 1 env throughout the course of infection. J. Virol., 80:10591-10599.
Kitrinos, K. M., J. A. E. Nelson, W. Resch, and R. Swanstrom. 2005. The effect of a protease inhibitor-induced genetic bottleneck on human immunodeficiency virus type 1 env gene populations. J. Virol. 79:10627-10637.
Nelson, J. A. E., F. Baribaud, T. Edwards, and R. Swanstrom. 2000. Patterns of Changes in Human Immunodeficiency Virus Type 1 V3 Sequences Late in Infection. J. Virol. 74:8494-8501.