Welcome to the UNC Flow Cytometry Core Facility

Facility overview

The UNC Flow Cytometry Facility provides state-of-the-art flow cytometry and related services to the entire UNC-CH research community as well as to others in the Research Triangle Park area. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves at reduced cost. A major part of our mission is to teach this technology to investigators, students, and staff. Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data. The core is located in the Department of Microbiology & Immunology, on the 6th floor of the Mary Ellen Jones Building, 116 Manning Drive, Chapel Hill, NC. Directions.

Instrumentation

Analytic Cytometers Cell Sorting
Three 9-color Beckman CyAns
Beckman MoFlo Legacy
18-color BD LSR II with HTS Module Beckman MoFlo XDP - 5-Laser Propel Colase upgrade
Cytek-modified , 5-color FACS Calibur in BSL-2 Containment BSL-2 cell sorting:

Stratedigm S1000Ex for Microparticle analysis: in BSL-2 containment. Now located in Hematology lab 307 MEJB. Contact: mmooberr@unch.unc.edu for access.

FACS ARIA SORP 4-laser system including 96-well sorting and cell indexing.  BSL-2 containment

Facility fees: https://www.med.unc.edu/flowcytometry/access/rates-policies-rules/facility-fees 

Did you know?

High Throughput sample screening can be done on our LSR II! There is a 96-well HTS unit that will let you run 96 samples at a time without having to change a tube! Diva software allows you to pre-program well contents for efficient data analysis. This is a real time saver for any flow cytometry experiment with lots of tubes to run.

The Flow Cytometry Core Facility hosts a number of analysis software options including specialty software on our Analysis computer in room 618 MEJB and access to our FlowJo site license. See our web page https://www.med.unc.edu/flowcytometry/software-for-acquisition-and-analysis/available-software or ask our staff for more information!

Updated Compensation tips on our webpage: http://www.med.unc.edu/flowcytometry/basic-flow-cytometry/compensation. See also the FlowJo 'Daily Dongle' http://flowjo.typepad.com/the_daily_dongle/2011/09/index.html for FlowJo- specific instructions.

New to sorting? See our updated Sorting Tips: A Guide for Investigators, with detailed information that you should know while planning your sorting experiment and communicating with our staff.

Are you planning on publishing your Flow Cytometry Data? New publication requirements are coming (or are already enforced) for many high impact journals. For a description of MIFlowCyt - 'Minimum Information for Flow Cytometry experiments', view this link: http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20623/pdf For now, see our link under 'Application Protocols' for instructions on running Calibration Beads.

Lineberger Cancer Comprehensive Center Members, please include in your publication acknowledgements: 'The UNC Flow Cytometry Core Facility is supported in part by an NCI Center Core Support Grant (P30CA016086) to the UNC Lineberger Comprehensive Cancer Center.' Also : All cancer center members publishing data generated in the NIH-supported Flow Cytometry Core Facility are required to comply with the NIH Public Access Policy, http://publicaccess.nih.gov/

For all publications resulting from use of the Stratedigm, please include in the acknowledgements: 'Research reported in this publication was supported by the Office of the Director, National Institutes of Health under award number S10OD012052. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.'

For all publications resulting from use of the FACS ARIA should include in the acknowledgements: 'Research reported in this publication was supported in part by the North Carolina Biotech Center Institutional Support Grant 2012-IDG-1006'

 


Tuesday, June 19, 2012 11:00 AM – 1 PM Research Triangle Cytometry Association Meeting: Seeing is Believing: Image Based Cytometry of Cultured Cells Carl Bortner, PhD, Staff Scientist NIEHS/NIH, Research Triangle Park, North Carolina and Cytometry with Vision: Research Applications on the Amnis FlowSight Imaging Flow Cytometer Karen R. Tamul, MS, MT(ASCP)SI, Instrument Field Application Scientist Bioscience Division, EMD Millipore Lunch will be provided. Please RSVP to Robert Bunzelso we can get an accurate head count. (Robert.Bunzel@merckgroup.com) US EPA-RTF Building (formerly NHEERL) Room 1405 2525 NC Hwy 54, Research Triangle Park, North Carolina 27711 The RTF building is a small red brick building located close to the corner of Alexander Drive and NC 54. For information about this meeting contact: Robert Zucker Zucker.Robert@epamail.epa.gov (919) 541-1585 . The RTCA gratefully acknowledges EMD Millipore for reimbursing the expenses of this meeting. For information about the Research Triangle Cytometry Association contact: Marie Iannone marie.a.iannone@gsk.com (919) 483-9486