Flow Cytometry for Aquatic Sciences

The 5-day course will include both lecture and laboratory sessions, and provides aquatic and environmental scientists and/or commercial entities an introduction to flow cytometry, including the theory of operation as well as an introduction to different flow cytometric instrumentation (analyzers and BD Influx cells sorters) including imaging cytometry (FlowCAM). Breakfast and lunch are provided each day and the course will also include one group dinner (Maine Lobster Bake). On campus, lodging is included with course fees.

When Sep 25, 2017 09:00 AM to
Sep 29, 2017 05:00 PM
Where East Boothbay, Maine
Contact Name
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 Course information and Registration:


 This course is organized by Dr. Nicole Poulton, Director of the J.J. MacIsaac Facility for Aquatic Cytometry (https://fac.bigelow.org) and will include additional lecturers who are experts within their fields.   Registration is limited to 10 participants. 

Throughout the week topics will include discussions on the wide number of applications within the aquatic sciences including, but not limited to:

 1. Culture & Environmental sample enumeration (viruses, bacteria, phytoplankton and heterotrophic protists), including preservation techniques.

 2. Cell Sorting for Biogeochemical analyses (C, N, & P)

 3. Cell sorting for isolation and cultivation from environmental samples.

 4. Single cell sorting for whole genome amplification (WGA) and genome analysis.

 5. Imaging Cytometry for Aquatic applications (using FlowCAM).

  Guest lecturers include:

Ger van den Engh, Ph.D., - Center for Marine Cytometry (marinecytometry.space)

 Michael Lomas, Ph.D., - Director of the National Center for Marine Algae and Microbiota (https://ncma.bigelow.org)

 Ramunas Stepanauskas, Ph.D., Director of the Single Cell Genomics Center (https://scgc.bigelow.org)

 Bigelow Laboratory for Ocean Sciences investigates the microbial drivers of global ocean processes through basic and applied research, education and enterprise.  Research at Bigelow Laboratory is organized around three core themes, Blue Biotechnology, Ocean Biogeochemistry and Climate Change, and Ocean Health.  In 2012, the Laboratory's 60,000 sq ft, shore-based campus in East Boothbay, Maine became the first LEED Platinum building in Maine and one of seven in the New England area.

 For questions or additional information, or specific aquatic application requests, please contact Facility Director, Dr. Nicole Poulton: npoulton@bigelow.org


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