BD/Cytek FACSCalibur - For BSL-2 Analysis
A Becton-Dickinson FACSCalibur is available to provide analytic capability. The FACScan has 7parameter analysis capability - forward and side scatter and 5 colors of fluorescence using 488 and 635nm excitations. The standard FACSCalibur has been modified by Cytek Development to add a better red laser and a 5th color. The instrument currently uses a PC computer for instrument control and data acquisition. The software running the FACSCalibur is FlowJo CE. The FACScan is available for use by trained investigators/staff or Facility staff will run your samples. Biohazardous samples are not permitted on the FACSCalibur. Any samples that potentially or are known to contain biohazard agents must be fixed. This includes human cells from any source.
BSL-2 containment: lab coat, shoes, eye protection, and BSL-2 training required. Laboratory Schedule F and Biohazard Form should be sent to the facility director before using BSL-2 material in the core facility.
Location: 613 Mary Ellen Jones Bldg.
488nm and 637nm excitation
5 colors of fluorescence
Possible Fluorochromes to Use
488nm excitation - (blue laser)
FL1 - (530/30) FITC, Alexa488, GFP, YFP
FL2 - (585/42) R-PE, PI, DsRed
FL3 - (688 LP) PerCP, PE-CY5, PE-Cy5.5, PE-Alexa 647, PI, 7AAD, DRAQ5
637nm excitation - (red laser)
FL4 - (666/27) APC, Alexa 647, To-Pro3
FL5 - (740 LP) APC-Cy7, APC-Alexa 750
- The Calibur's two lasers are 30 mW 488 Diode Pumped Solid State (DPSS) laser and a 25 mW 637 red diode laser.