Mo Flo XDP - 5 Laser Colase system

Beckman-Coulter (Dako) MoFlo XDP - Now 5 lasers with our new Propel Colase Upgrade!! 

The Dako MoFlo XDP is the latest generation MoFlo sorter. It retains the excellent MoFlo fluidics system but integrates this with a fully digital electronics configuration. The MoFlo XDP is equipped with 3 lasers to provide a number of useful excitation wavelengths. The primary laser is a Coherent/iCyt 200mW Sapphire diode-pumped solid-state laser. It is used for 488nm excitation. A Melles-Griot 35mW Helium-Neon laser producing 633nm is available for the red excitable fluorochromes. The third laser is a 25mW Coherent Radius solid state violet (408nm) laser. The 633 laser is now colinear with the violet and we added a green (532 nm) laser colinear with UV (355 nM). 

The MoFlo XDP has the same excellent cloning device as the MoFlo for depositing single (or multiple) cells into multi-well culture plates from 6 well to 384 well configuration. The MoFlo XDP can sort 1-4 populations simultaneously. Our current configuration provides for up to 10 fluorescence signals. The data is available as true 5 decade log data. The MoFlo XDP is capable of high-speed sorting - for small cells (e.g. lymphocytes) input speeds can be in excess of 25,000/sec. - for larger cells input speeds can be up to around 10,000/sec. Actual speed available depends not only on size but other factors.

Instrument Overview

Location: 629 Mary Ellen Jones Bldg.
Laser Excitations: 408nm, 488nm, 633nm, 532 nm and 355 nm

  • 10 colors of fluorescence simultaneously
  • high speed sorting
  • culture plate deposition - single/multiple cell
  • 4-way sorting

Laser/emission and Possible Fluorochromes (not inclusive):

violet/408nm - Pacific Blue, Pacific Orange, DAPI, Violet DyeCycle
Live/Dead violet, Quantum Dots

blue/488nm - FITC, Alexa 488, GFP, PE-Cy5.5, PE-TxRed, PE-Alexa610, PE-Cy5,
PE-Alexa700, PE-Alexa 750, PE-Cy7, PE-Alexa 647, DsRed

red/640nm  - APC, APC-Cy7, APC-Alexa 750, Alexa-647

green/532 nm -  PE/dTomato, mCherry/PE-TxRed/PI, mKate/PE-A647 (also supporting PE-Cy5, PE-A700 or PE-Cy7)

UV/355 nm - Alexa350/DAPI/Hoescht Blue, QD605/Hoescht Red, QD625

 

                Mo Flo XDP