Cell Filtration Options

There are several options for filtering your cells before running on a flow cytometer.

Compiled by Ann George, Flow Cytometry Core Facility, Children's Hospital, Los Angeles and shared on the Purdue List Serve on 6/2/2013

Commercially available filters:

1. 50 ml tube  Falcon filters from Fisher, product number is 08-771-2. Use
a different section of the filter for each sample and pipette slowly.

2. Partec filters sterile and non sterile: http://www.partec.com/reagents-accessories/accessories/celltricsr.html

You can pull the partec cell filters apart, clean them, replace the nylon mesh and reautoclave.  Great job for an undergrad…

3. Filters from YSL Bioprocess Development Co., soon to be on the market. www.yslbio.com

Many users had innovative ways to use mesh bought by the yard from the vendors below. Recommendations ranged from cutting the mesh into pieces and sterilizing with 70% ethanol, and then holding  it over a tube with
forceps, to  sandwiching the mesh between blue pipette tips .

Blue tip and mesh filtration unit:

http://www.vetmed.auburn.edu/uploads/32/94/3294070a7593b63647a94eac8d806fcb/flow_filtration.pdf

Another user cut a nylon mesh to 1x1 cm pieces and placed it between a syringe and a needle.

Other ways to use mesh by the yard: https://docs.google.com/viewer?a=v&pid=gmail&attid=0.1&thid=13eb2d0fbd9b2796&mt=application/msword&url=https://mail.google.com/mail/u/0/?ui%3D2%26ik%3D4300c56823%26view%3Datt%26th%3D13eb2d0fbd9b2796%26attid%3D0.1%26disp%3Dsafe%26zw&sig=AHIEtbSKOWdNP9v0aDbpvYe8M7w8pEoRYw


I thought this was very cost effective and efficient: Use the bulk mesh and
pipet through into 96 well plates - tape the mesh to the plate


Mesh vendors:

1. Millipore nylon mesh filter: http://www.millipore.com/catalogue/item/ny4100010

2. Amazonsite: http://www.amazonsupply.com/s/16414371?searchBinNameList=material_browse&field_material_browse=43805011&ref_=sp_iss_16414371


3. COMPONENT SUPPLYCOMPANY, Nylon Mesh 44 Micron, 27% open Area 1 YARD
Catalog No.: U-CMN-44-C  #38.82/yard.
http://www.componentsupplycompany.com/product_pages/Nylon-screening-mesh.html?gclid=CMWj8PmombcCFYFQ7AodGCIAUw

4. NITEX 30um 20%OA x 115cm, Nylon Mesh. Micron Rating: 30, Mesh Width:
115cm, from an Australian Company called SEFAR. (Stock Code: 03-30/20X115CM)

5. http://www.elkofiltering.com/store/p/420-Nylon-Mesh-50-Micron.aspx

6. http://www.cardinal.com/us/en/distributedproducts/ASP/P5194-1.asp?cat=laboratory

7. Nylon mesh for hybridization ovens (VWR cat#47746-106, 47746-104),
https://us.vwr.com/store/catalog/product.jsp?catalog_number=47746-104

Link to a previous thread on the Purdue list:

https://lists.purdue.edu/pipermail/cytometry/2011-March/thread.html#40950

 

From Bill Godfrey , Beckman Coulter: through Purdue List 6/2/2013:

There is an old trick of melting a piece of nytex on the end of a pipet tip. Heat up a hot plate just enough to melt the tip, but not the nytex. Cut off a P-200 or P-1000 tip with a razor blade to provide a larger opening. Put the nytex on the plate and press the pipet tip on it long enough to melt the tip onto the nytex along the entire circumference, then remove. The filter tips can be autoclaved. Can pipet cells into the tip and then force through with a pipettor if necessary.