Useful Flow Cytometry Links

Weblinks for Spectral Viewers, more in-depth tutorials, methods and tools.

Join ISAC! The International Society for the Advancement of Cytometry: www.isac-net.org

SPECTRAL VIEWERS:

BDBioscienceshttp://www.bdbiosciences.com/research/multicolor/spectrum_viewer/index.jsp

Invitrogen: http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html
Note: the main difference between these are the specific reagents that each company sells.

eBioscience FluorPlan: http://www.ebioscience.com/resources/fluorplan-spectra-viewer.htm   Customizable to various instrument configurations and multiple laser lines.

Biolegend Spectra Analyzerhttp://www.biolegend.com/spectraanalyzer

Chroma - http://www.chroma.com/products/fluorochrome

Spectral viewers are available for your mobile devises from Invitrogen (SpectraViewer), Biolegend and more. 

Panel Builder  http://www.fluorish.com/

Table of Fluorescent Proteins - excitation, emission and quantum efficiencies:   http://nic.ucsf.edu/dokuwiki/doku.php?id=fluorescent_proteins

Excyte Blog on Statistics and Data analysis:  http://expertcytometry.com/category/statistics-and-data-analysis/   Tips on data analysis, gating, T-tests and links to other best practices and definitions. 

Fluorophore Guide:

BD Multicolor Fluorochrome Guide: http://www.bdbiosciences.com/documents/Multicolor_Fluorochrome_Guide.pdf

Dartmouth staining index guide showing relative brightness of various fluorophores on several different instruments: http://www.dartmouth.edu/~dartlab/index.php?page=anti-cd8-stain-index

Practical Flow Cytometry online Text: Download Howard Shapiro's 'Practical Flow Cytometry'  for online viewing and mobile viewing at:  http://bit.ly/18jrQsS

TUTORIALS:

 Check out CYTO UNIVERSITY!!!  http://cytou.org/store/provider/provider09.php  

Things you should know about Autofluorescence! Autofluorescence: Causes and Cures

MUST-SEE webinar from Kelly Lunsten at Biolegend on understanding Fluorophores and Panel Design:  http://www.biolegend.com/brilliantviolet_webinar

Chromocyte

Tips for good flow cytometry experiments  http://www.chromocyte.com/educate/Beginners-Guide-Training-Resources/Tips-for-Good-Flow-Cytometry-Experiments

Webinars: http://www.chromocyte.com/educate/Beginners-Guide-Training-Resources/Webinars


Introduction to Flow Cytometry:

http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html

Webinars from BD on Apoptosis, Cell Signaling, Stem Cells, T-Cell Immunology, Bead Based Immunoassays: http://www.bdbiosciences.com/hotlines/webinars/archives/

From Beckman website: https://www.beckmancoulter.com/wsrportal/wsr/support/training-education/research-webinars-conferences/index.htm

  • For CyAns and Summit Software:

http://www.coulterflow.com/bciflow/theory.php

  • For LSR II and DIVA:

http://www.bdbiosciences.com/support/training/itf_launch.jsp

  • More general flow cytometry basics:

www.invitrogen.com/site/us/en/home/support/Tutorials.html

USEFUL ARTICLES AND LINKS:

If you are new to cell sorting experiments, see our page on Practical Cell Sorting - a guide for investigators

For experienced flow cytometry users, check out Ryan Duggan's blog from the University of Chicago Flow Cytometry lab: http://ucflow.blogspot.de/ - there are great discussions about Annexin gating, MFI, log transformation, compensation and more!

 BD Website tutorials for Flow Cytometry overview:
http://www.bdbiosciences.com/support/training/self_paced.jsp

DIVA software training (works only on Explorer or Netscape):

http://www.bdbiosciences.com/services/training/elearning/index.jsp

Interpreting flow cytometry data: a guide for the perplexed. Herzenberg et al. 2006 Nature Immunology 7:681 http://www.nature.com/ni/journal/v7/n7/full/ni0706-681.html

Introduction to Fluorescent Proteins: Nikon MicroscopyU article with spectral profiles of common fluorescent proteins: www.microscopyu.com/articles/livecellimaging/fpintro.html

Flow Cytometry – A Basic Introduction
http://flowbook.denovosoftware.com/

Beckman Coulter Webinar Series - Learn to operate the CyAn and Summit software:
http://www.coulterflow.com/bciflow/webinar.php

Purdue Flow Cytometry www.cytometry.purdue.org
Join the Purdue Flow Cytometry List Serve to get in on the chatter of current flow issues and concerns by users over the globe.

Giving a seminar:
additional teaching slides at:  http://www.isac-net.org/

Several good primers on flow cytometry are provided by instrument and antibody manufacturers:

www.bdbiosciences.com/support/training/itf_launch.jsp

www.bdbiosciences.com/support/training/self_paced.jsp

www.invitrogen.com/site/us/en/home/support/Tutorials.html

Another primer is Michael G Oremond's  Flow Cytometry - A Basic Introduction:

flowbook.denovosoftware.com

METHODS

Current Protocols in Cytometry - we have a facility subscription, so let us know if you need any methods from this site.
http://http://onlinelibrary.wiley.com/book/10.1002/0471142956

 

Free Analysis Software: 

Chromocyte software list: http://www.chromocyte.com/educate/Flow-Cytometry-Instruments-Software--Upgrades/Flow-Cytometry-Software

Cytospec: http://www.cyto.purdue.edu/Purdue_software  There are a stack of tutorials on that page to show you how to use it.

 Cyflogic:  http://www.cyflogic.com/index.php

Flowing: http://www.flowingsoftware.com/

On-line lymphoid slide ruler:  It is a quick and easy way to review what markers should be positive and negative for numerous disease states involving T, B and NK cells.   http://www.coulterflow.com/bciflow/slideruler.php