Join ISAC! The International Society for the Advancement of Cytometry: www.isac-net.org
Note: the main difference between these are the specific reagents that each company sells.
eBioscience FluorPlan: http://www.ebioscience.com/resources/fluorplan-spectra-viewer.htm Customizable to various instrument configurations and multiple laser lines.
Biolegend Spectra Analyzer: http://www.biolegend.com/spectraanalyzer
Chroma - http://www.chroma.com/products/fluorochrome
Spectral viewers are available for your mobile devises from Invitrogen (SpectraViewer), Biolegend and more.
Panel Builder http://www.fluorish.com/
Table of Fluorescent Proteins - excitation, emission and quantum efficiencies: http://nic.ucsf.edu/dokuwiki/doku.php?id=fluorescent_proteins
Excyte Blog on Statistics and Data analysis: http://expertcytometry.com/category/statistics-and-data-analysis/ Tips on data analysis, gating, T-tests and links to other best practices and definitions.
BD Multicolor Fluorochrome Guide: http://www.bdbiosciences.com/documents/Multicolor_Fluorochrome_Guide.pdf
Dartmouth staining index guide showing relative brightness of various fluorophores on several different instruments: http://www.dartmouth.edu/~dartlab/index.php?page=anti-cd8-stain-index
Practical Flow Cytometry online Text: Download Howard Shapiro's 'Practical Flow Cytometry' for online viewing and mobile viewing at: http://bit.ly/18jrQsS
Check out CYTO UNIVERSITY!!! http://cytou.org/store/provider/provider09.php
Things you should know about Autofluorescence! Autofluorescence: Causes and Cures
MUST-SEE webinar from Kelly Lunsten at Biolegend on understanding Fluorophores and Panel Design: http://www.biolegend.com/brilliantviolet_webinar
Tips for good flow cytometry experiments http://www.chromocyte.com/educate/Beginners-Guide-Training-Resources/Tips-for-Good-Flow-Cytometry-Experiments
Introduction to Flow Cytometry:
Webinars from BD on Apoptosis, Cell Signaling, Stem Cells, T-Cell Immunology, Bead Based Immunoassays: http://www.bdbiosciences.com/hotlines/webinars/archives/
- For CyAns and Summit Software:
- More general flow cytometry basics:
USEFUL ARTICLES AND LINKS:
If you are new to cell sorting experiments, see our page on Practical Cell Sorting - a guide for investigators
For experienced flow cytometry users, check out Ryan Duggan's blog from the University of Chicago Flow Cytometry lab: http://ucflow.blogspot.de/ - there are great discussions about Annexin gating, MFI, log transformation, compensation and more!
BD Website tutorials for Flow Cytometry overview:
DIVA software training (works only on Explorer or Netscape):
Interpreting flow cytometry data: a guide for the perplexed. Herzenberg et al. 2006 Nature Immunology 7:681 http://www.nature.com/ni/journal/v7/n7/full/ni0706-681.html
Introduction to Fluorescent Proteins: Nikon MicroscopyU article with spectral profiles of common fluorescent proteins: www.microscopyu.com/articles/livecellimaging/fpintro.html
Flow Cytometry – A Basic Introduction
Beckman Coulter Webinar Series - Learn to operate the CyAn and Summit software:
Purdue Flow Cytometry www.cytometry.purdue.org
Join the Purdue Flow Cytometry List Serve to get in on the chatter of current flow issues and concerns by users over the globe.
Giving a seminar: additional teaching slides at: http://www.isac-net.org/
Several good primers on flow cytometry are provided by instrument and antibody manufacturers:
Another primer is Michael G Oremond's Flow Cytometry - A Basic Introduction:
Current Protocols in Cytometry - we have a facility subscription, so let us know if you need any methods from this site.
Free Analysis Software:
Chromocyte software list: http://www.chromocyte.com/educate/Flow-Cytometry-Instruments-Software--Upgrades/Flow-Cytometry-Software
Cytospec: http://www.cyto.purdue.edu/Purdue_software There are a stack of tutorials on that page to show you how to use it.
On-line lymphoid slide ruler: It is a quick and easy way to review what markers should be positive and negative for numerous disease states involving T, B and NK cells. http://www.coulterflow.com/bciflow/slideruler.php