Confocal Laser Scanning Microscopy

Reading Assignment:

Path 464 Class notes CLSM Tutorial v2, Ch-13 CLSM

 

Introduction to Confocal Microscopy.pdf (or http://www.olympusmicro.com/primer/techniques/confocal/confocalintro.html)

 

Airy Disk demonstration at http://www.olympusmicro.com/primer/java/microscopy/airydiscs2/index.html

 

Optical sectioning demonstration at http://zeiss-campus.magnet.fsu.edu/tutorials/opticalsectioning/confocalwidefield/index.html

 

Two Lenses Together

The scanning principal – Rasters and ROIs

Resolution in the Z axis        See: Resolution in CLSM.pdf
(or http://www.olympusmicro.com/primer/techniques/confocal/resolutionintro.html)

 

Detectors – QE, bit-depth, speed     See: Hamamatsu PMT handbook.pdf

 

Data storage

 

[Zip Package of all Section 5 Documents]