Confocal Laser Scanning Microscopy

Reading Assignment:

Path 464 Class notes CLSM Tutorial v2, Ch-13 CLSM


Introduction to Confocal Microscopy.pdf (or


Airy Disk demonstration at


Optical sectioning demonstration at


Two Lenses Together

The scanning principal – Rasters and ROIs

Resolution in the Z axis        See: Resolution in CLSM.pdf


Detectors – QE, bit-depth, speed     See: Hamamatsu PMT handbook.pdf


Data storage


[Zip Package of all Section 5 Documents]