Equipment Descriptions

Zeiss Leo 910 TEM
Zeiss TEM 910 Transmission Electron Microscope

40 kV to 120 kV accelerating voltage
0.4 nm resolution
Eucentric goniometer - computer controlled in X, Y, Z, and tilt
Digital imaging interface utilizing a Gatan SC1000 camera

Zeiss Supra 25 FESEM
Zeiss Supra 25 Field Emission Scanning Electron Microscope

Resolution: 2 nanometers
Accellerating voltage: 1 kV to 30 kV
Magnification 20X – 1,000,000X
Fully computer controlled with digital image capture
Motorized goniometer stage will hold up to 8 samples
In-lens secondary electron detector
Standard T-E secondary electron detector
Backscatter detector
STEM detector
EDX detector

Zeiss LSM 710
Zeiss CLSM 710 Spectral Confocal Laser Scanning Microscope

Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
Two conventional and one spectral channels and one transmitted light channel
Excitation lines: 405, 458, 488, 514, 543, 594, 633
Fully motorized with motorized stage and Definite Focus
Capable of automated z-series, time-lapse, xyzt, and
spectral image capture
Software for linear unmixing, FRAP, FRET, Co-localization
Zeiss PECON incubation system for live cell imaging with temperature and CO2 control
Objective Lenses: 5X/0.16 Plan Apo, 10X/0.45 Plan Apo, 20X/0.80 Plan Apo, 40X/0.95 Plan Apo
25X/0.80 Plan Apo Multi Immersion, 40X/1.3 Oil Plan Neo, 63X/1.4 Oil Plan Apo

Zeiss LSM 700
Zeiss CLSM 700 Confocal Laser Scanning Microscope

Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
Two conventional channels and one transmitted channel
Excitation lines: 405, 488, 555, 639 nm
Motorized stand and motorized stage and Definite Focus
Capable of automated z-series, time-lapse, xyzt, and tiling
Software for FRAP, FRET, Co-localization, etc.
Bioptechs open dish live cell imaging system
Objective lenses: 5X/0.16 Plan Apo, 10X/0.45 Plan Apo, 20X/0.80 Plan Apo
40X/0.6 LD Plan Neo CC, 40X/1.4 Plan Apo Oil, 63X/1.4 Plan Apo Oil

Olympus FV500
Olympus FV500 Confocal Laser Scanning Microscope

Inverted CLSM with DIC controlled by Olympus FLUOVIEW software
Three confocal channels and one transmitted light channel
Excitation lines: 458, 488, 514, 543, and 633
Fully motorized with manual stage
Capable of automated z-series, time-lapse, and xyzt image capture
Bioptechs open dish live cell imaging system
Objective Lenses: 4X/0.13 UPlanFLN, 10X/0.40 UPlan Apo, 20X/0.50 UPlanFL
40X/1.00 - 0.5 UPlan Apo Oil w/Iris, 60X/1.4 Plan Apo Oil

Olympus BX61
Olympus BX61 Upright Wide Field Microscope

Imaging modes: Bright Field, Dark Field, DIC,
Polarized Light, Epi-fluorescence
Fully motorized with manual stage
Computer controlled by Improvision’s Velocity software
Capable of automated time lapse and z-series
Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
Objective Lenses: 4X/0.13 UPlanFLN, 10X/0.30 UPlanFLN, 20X/0.50 UPlanFLN,
40X/1.3 Oil UPlanFLN, 60X/1.42 Oil PlanApo N, 100X/1.30 Oil UPlanFLN

                       Olympus IX81
Olympus IX81 Inverted Light Microscope

Imaging modes: Bright Field, Phase, DIC, Epi-fluorescence
Fully motorized including LEP Biopricision motorized stage
Computer controlled by Improvision’s Velocity software
Capable of automated time lapse, z-series and multiple x-y
position image capture
Zero Drift Control maintains focus over space and time
Bioptechs open dish live cell system
Photonic Mosaic photo-bleach system
Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
Objective Lenses: 1.25X/0.04 PlanApoN, 2X/0.08 PlanApoN
4X/0.13 PhL UPlanFLN, 10X/0.30 Ph1 UPlanFLN
20X/0.45 Ph1 LUCPlanFLN, 40X/0.60 Ph2 LUCPlanFLN
40X/1.30 Oil DIC UPlanFLN, 60X/1.42 Oil DIC PlanApo
100X/1.3 Oil UPlanFLN

Olympus IX70
Olympus IX70 Inverted Live Cell System

"Scope In A Box"
Imaging modes: Bright Field, Phase, Epi-fluorescence
Motorized stage and motorized focus control with
anti-drift correction
Enclosed within an environmental chamber controlling temperature,
relative humidity and CO2

Computer controlled utilizing Improvision’s Volocity software
Capable of time-lapse imaging at multiple locations in
phase and two fluorescence colors over several days
Objective Lenses: 4X/0.13 PhL UPlanFL, 10X/0.30 Ph1 UPlanFL
20X/0.40 Ph1 LCPlanFL, 40X/0.60 Ph2 LCPlanFL

Zeiss PALM
Zeiss PALM Microdissection System

A fully computer controlled microdissection system built on a
Zeiss Axiovert fluorescence microscope stand.
Sections of tissue are placed on special membrane coated
slides. Areas for microdissection are selected on a computer
monitor in either bright field or fluorescence light using

various drawing tools after which microdissection is carried
out automatically. The special membrane plus tissue is cut
through in
the selected areas using a nitrogen UV laser and areas are
lifted from the slide into a waiting receptacle through a
laser catapulting method.
Cutting Lenses: 5X/0.25 FLUAR, 10X/0.50 FLUAR
20X/0.40 Ph2 Korr LD Plan-Neofluar, 40X/0.60 Korr LD Plan-Neofluar
60X/0.75 Korr LD Plan-Neofluar

Arcturus LCM
Arcturus PixCell II Laser Capture Microdissection System

The PixCell II is built on an Olympus IMT 2 inverted stand.
Imaging modes: bright field and epi-fluorescence.
Special hardware captures individual cells onto a plastic membrane
held on a cap that fits a 0.5 mm Eppendorf tube. The associated computer
system and Arcturus software can
capture images from the LCM, provide
laser pulse counts and other useful data.
Objective lenses: 4X/0.13 UPlanFl, 10X/0.30 UPlanFl, 20X/0.40 LCPlanFl
40X/0.60 LCPlanFl

WILD M420
Leica WILD Macroscope M420

Macroscopic imaging system for objects up to 5 cm long
Magnification from 3.6X to 87.5X with APOZOOM lens
Bright field and dark field imaging in transmitted light
Epi-illumination (not fluorescence) imaging in reflected light
QImaging Micropublisher Camera using QCapture software