Equipment list

Light microscopy

Zeiss LSM 710 Spectral Confocal Laser Scanning Microscope

Capable of automated z-series, time-lapse, xyzt, and spectral image capture.

Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
Two conventional and one spectral fluorescence channel and one transmitted light channel
Motorized stage with Definite Focus for automatic focus drift correction
Zen software for linear unmixing, FRAP, FRET, Co-localization

Zeiss PECON incubation system for live cell imaging with temperature and CO2 control, compatible with 35mm dishes and chambered coverglass slides

Excitation lines:

  • 405 nm
  • 458 nm
  • 488 nm
  • 514 nm
  • 543 nm
  • 594 nm
  • 633 nm

Objective Lenses:

  • 5X/0.16 Plan Apo
  • 10X/0.45 Plan Apo
  • 20X/0.80 Plan Apo
  • 40X/0.95 Plan Apo
  • 25X/0.80 Plan Apo Multi Immersion
  • 40X/1.3 Oil Plan Neo
  • 63X/1.4 Oil Plan Apo

 

Zeiss LSM 700 Confocal Laser Scanning Microscope

Capable of automated z-series, time-lapse, xyzt, and tiling.

Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
Two conventional fluorescence channels and one transmitted channel
Motorized stage with Definite Focus for automatic focus drift correctionZen software for FRAP, FRET, Co-localization, etc.

Bioptechs temperature control system for live cell imaging, compatible with 35mm Bioptechs 0400417B dishes.

Excitation lines:

  • 405 nm
  • 488 nm
  • 555 nm
  • 639 nm

Objective lenses:

  • 5X/0.16 Plan Apo,
  • 10X/0.45 Plan Apo
  • 20X/0.80 Plan Apo
  • 40X/0.6 LD Plan Neo
  • 40X/1.4 Plan Apo Oil
  • 63X/1.4 Plan Apo Oil

 

Lavision Ultramicroscope II light-sheet system

This system illuminates samples with a light-sheet that is approximately 5um-thick at its narrowest point in the Z dimension. To avoid shadowing artifacts and allow full imaging of thick samples, 3 angled light-sheets can illuminate the sample from each side. In addition, the light-sheet can be scanned horizontally in the X dimension to avoid a loss of Z resolution near the edges. The system comes equipped with five laser lines (488, 514, 561, 647, 785nm) and filter sets compatible with all common fluorophores from the green to far red regions of the spectrum. The system is outfitted with a 2X/0.5 objective coupled to a zoom body with a total of 11 possible magnifications, ranging from 1.26 to 12.6X. The objective has a corrected dipping cap with a working distance of 5.7mm, an alternative dipping cap with a working distance of 10mm (though at the cost of inferior optical quality) is available. These objectives and the sample mounting system are fully compatible with immersion media used in all clearing protocols, including those that require harsh organic solvents. The system has an automated stage that allows easy tiling of large specimens.

 

Olympus BX61 Upright Wide Field Microscope

Capable of automated time lapse and z-series

Imaging modes: Bright Field, Dark Field, DIC,
Polarized Light, Epi-fluorescence
Manual stage
Computer controlled by Improvision’s Velocity software

Hammatsu ORCA RC and QImaging RETIGA 4000R cameras

Objective Lenses:

  • 4X/0.13 UPlanFLN
  • 10X/0.30 UPlanFLN
  • 20X/0.50 UPlanFLN
  • 40X/1.3 Oil UPlanFLN
  • 60X/1.42 Oil PlanApo N
  • 100X/1.30 Oil UPlanFLN

 

Olympus IX81 Inverted Light Microscope

Capable of automated time lapse, z-series and multiple x-y position image capture, as well as targeted photobleaching, photoactivation and photostimulation

Imaging modes: Bright Field, Phase, DIC, Epi-fluorescence
Fully motorized including LEP Bioprecision motorized stage
Computer controlled by Improvision’s Velocity software
Capable of automated time lapse, z-series and multiple x-y position image capture
Zero Drift Control maintains focus over space and time

Bioptechs CO2 and temperature control system for live cell imaging, compatible with 35mm Bioptechs 0400417B dishes.
Andor Mosaic photo-bleach system

Hammatsu ORCA RC and QImaging RETIGA 4000R cameras

Objective Lenses:

  • 1.25X/0.04 PlanApoN,
  • 2X/0.08 PlanApoN
  • 4X/0.13 PhL UPlanFLN
  • 10X/0.30 Ph1 UPlanFLN
  • 20X/0.45 Ph1 LUCPlanFLN
  • 40X/0.60 Ph2 LUCPlanFLN
  • 40X/1.30 Oil DIC UPlanFLN,
  • 60X/1.42 Oil DIC PlanApo
  • 100X/1.3 Oil UPlanFLN

 

Olympus IX70 Inverted Live Cell System

Capable of time-lapse imaging at multiple locations in phase and three fluorescence channels over several days with stable focus, temperature and CO2 concentration.

Imaging modes: Bright Field, Phase, Epi-fluorescence
Motorized stage and motorized focus control
Enclosed within an environmental chamber controlling temperature and CO2

Stage compatible with multi-well plates, chambered coverglass slides, chambered slides, and 35 mm dishes.

Computer controlled utilizing cellSens software

Hamamatsu Flash 4.0 V2+ sCMOS camera.

Filters optimized for the following fluorophores and combinations of fluorophores:

  • Cy5
  • Rhodamine
  • GFP/mCherry
  • CFP/YFP/mCherry

Objective Lenses:

  • 4X/0.13 PhL UPlanFL
  • 10X/0.30 Ph1 UPlanFL
  • 20X/0.45 Ph1 LUCPlanFLN
  • 40X/0.60 Ph2 LUCPlanFLN

 

Leica WILD Macroscope M420

Macroscopic imaging system for objects up to 5 cm long

Magnification from 3.6X to 87.5X with APOZOOM lens
Bright field and dark field imaging in transmitted light
Epi-illumination (not fluorescence) imaging in reflected light

QImaging Micropublisher Camera using QCapture software

 

Electron Microscopy 

Zeiss LEO EM 910 Transmission Electron Microscope

40 kV to 120 kV accelerating voltage, tungsten filament
0.4 nm point resolution, high contrast OIS system
50X to 500,000X magnification range
Eucentric goniometer stage in X, Y, Z, +/- 30 degree tilt
Gatan Orius SC1000 CCD camera with Digital Micrograph 2.3.1 software

 

JEOL JEM 1230 Transmission Electron Microscope

40 kV to 120 kV accelerating voltage, LaB6 filament
0.2 nm lattice, 0.38nm point resolution, high contrast objective pole piece
50X to 600,000X magnification range
5-axis goniometer stage in X, Y, Z, +/- 45 degree tilt, 360˚rotation
Gatan Orius SC1000 CCD camera with Gatan Microscopy Suite 3.0 software

 

Zeiss Supra 25 Field Emission Scanning Electron Microscope

Resolution: 2 nanometers
Accelerating voltage: 1 kV to 30 kV
Magnification 20X – 1,000,000X
Fully computer controlled with digital image capture
Motorized goniometer stage will hold up to 8 samples
In-lens secondary electron detector
Standard T-E secondary electron detector
Backscatter detector
STEM detector
EDX detector

 

Image Analysis

Imaging workstation with the following software packages:

  • Imaris: 5D analysis
  • Autoquant: deconvolution
  • Metamorph: image analysis
  • Matlab: macros for image analysis
  • FIJI: free, open-source software for image analysis