Skip to the end of the utility bar
UNC School
of
Medicine
UNC Chapel Hill
UNC Health
Intranet
Login
Skip to main content
Search_for:
Search
Search this site
Search UNC School of Medicine
Toggle navigation
Mutant Mouse Resource and Research Centers at UNC
Home
Services
Resources
About Us
Contact Us
Publications
Home
/
MMRRC Services
/
UNC Cryogenics
/
Cryo Application
Cryo Application
Cryo Application
Select A Service
*
Sperm Cryopreservation
Embryo Cryopreservation
Sperm Cryorecovery
Embryo Cryorecovery
Is The Strain You Would Like To Submit Published?
*
Yes
No
You answered yes - the strain is published
Please visit www.mmrrc.org to apply for strain submission to our public repository. If accepted, there will be no fee for services. However, we will publish the strain as being available to the research community and we will take responsibility for distributing the strain to those requesting it. It is our policy, as well as NIH’s, that all published strains should be deposited in the public repository for research community use. For strains made by investigators from other institutions but imported into UNC, we suggest that you to contact the originating investigator to encourage them to submit the strain to the public archive. If for any reason this is not possible, we will consider archiving the strain for you in the UNC private archive.
Hidden
Date
MM slash DD slash YYYY
Investigator Name
*
First
Last
Investigator Email
*
Department
*
Lab Contact
*
First
Last
Lab Contact Phone
*
Lab Contact Fax
Lab Contact Email
Billing Contact Name
*
First
Last
Billing Contact Email
*
Billing Contact Phone
*
Campus Billing Address
*
IACUC Protocol #
PS Fund
*
PS Source
*
PS Dept
*
PS Project ID
*
PS PC Bus Unit
*
PS PC Activity
Account #
*
558913: Laboratory - Analytical Service
537210: Supplies - Educational/Research
Program Code
Cost Code 1
Cost Code 2
Cost Code 3
Strain Name
*
Genetic Background Strain
*
Affected Gene(S)
*
Briefly Describe The Genetic Alterations
Strain Development
Please describe the breeding history from original mutant mouse to the current background
Briefly Describe The Phenotype Related To The Mutation
Genotype
*
Targeted Mutation
Transgenic
Induced Mutant
Coat Color
*
Eye Color
*
General Disclaimer:
It is recommended that at least 2 males (10-24 weeks of age) be submitted for sperm cryopreservation. Males need to be housed separately at least 7 days before transfer to our facility. Sperm will be cryopreserved within 2 weeks of receiving the animals. The MMRRC at UNC will cryopreserve at least 25 straws (0.25cc Cassou straws) of sperm (10uL per straw). The MMRRC at UNC cannot make any guarantees that this procedure will be successful. Sperm cryopreservation is highly variable between strains as well as with individual mice. If the QC results are less than satisfactory, the MMRRC at UNC can repeat sperm cryo for an additional fee of $375. The MMRRC at UNC will perform Quality Control in vitro Fertilization to assess the quality of the sample. For an additional fee of $1000, the MMRRC at UNC can perform a QC IVF and transfer embryos to generate live pups. If this option is chosen and pups are born, the surrogate will be health tested. Due to the strict health requirements of our colony, we require that every surrogate be sent to RADIL for health testing. The cost of health testing is included. After weaning the pups, the surrogate will be sent for testing. It can take up to 3 weeks to get results back. Only after we have received the results can the pups be transferred to your colony (about 16 weeks after transfer). If genotyping confirmation is necessary, we can take tail snips when pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Number Of Mice Being Submitted
*
we recommend at least 2 males
Date Of Birth
*
YYYY slash MM slash DD
we recommend that mice be 10-24 weeks old
Genotype Of Mice Being Submitted
Location Of Mice
*
Building and Room #
Health Status Of Mice
Sample Storage
MMRRC Storage: (If choosing storage at the MMRRC facility, there is a $200/year storage fee. This fee is pro-rated for the first year and invoiced at the time of cryopreservation.)
Self-Storage/Investigator Lab: (If choosing self-storage, straws will be held at the MMRRC facility until a QC in-vitro fertilization (IVF) is performed. Investigators are responsible for collecting their sample within 30 days of the date of QC, otherwise the sample will automatically be stored with the MMRRC and the investigating lab’s grant will be billed. If you leave the University without notifying us, we have the right to dispose of your samples.
All samples are preserved in Cassou insemination straws and must be stored directly in liquid nitrogen or in liquid nitrogen vapor. Samples cannot be stored in any other type of freezer.
QC Options
*
QC Thaw/IVF (no charge/included in cryo fee) - MMRRC at UNC performs a small IVF with QC straw of sperm to access quality of the sample. The fertilization rate is recorded and all embryos produced are cultured to blastocyst stage.
QC to pups ($1000) - MMRRC at UNC performs a small IVF with QC straw of sperm to access quality of the sample. The fertilization rate is recorded and embryos produced are transferred to a pseudo-pregnant female. If pups are born, the surrogate will be health tested after pups are weaned. Due to the strict health standards of our colony, every surrogate that births must be sent to RADIL for full health testing. Generally, it takes about 16 weeks until mice are ready to be transferred to your colony. If genotyping confirmation is necessary, we can take tail snips when the pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Additional Comments
Any additional information that you would like to include with this application
Embryo Cryopreservation Service General Disclaimer
It is recommended that 2 males (10-24 weeks of age) and at least 10 females (7-12 weeks of age) be submitted for embryo cryopreservation. Males need to be housed separately at least 7 days before transfer to our facility. An IVF will be done within 2 weeks of receiving the animals, and the resulting embryos will be cryopreserved. The MMRRC at UNC recommends that 150-200 embryos be cryopreserved for the proper archival of the strain. This can take multiple attempts. IVF success is highly variable and depends on the number of female mice, the quality of the sperm and the fertilization rate. The MMRRC at UNC cannot make any guarantees that this procedure will be successful. In vitro Fertilization(IVF) and embryo cryopreservation can be highly variable between strains as well as with individual mice. The MMRRC at UNC will perform a quality control thaw by thawing a designated QC straw and culturing the embryos to blastocyst stage. For an additional fee of $1,000, the MMRRC at UNC can thaw and transfer embryos to generate live pups. Due to the strict health requirements of our colony, we require that every surrogate be sent to RADIL for health testing. The cost of health testing is included. After weaning the pups, the surrogate will be sent for testing. It can take up to 3 weeks to get results back. Only after we have received the results can the pups be transferred to your colony (generally takes 16 weeks). If genotyping confirmatin is necessary, we can take tail snips when pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Number Of Male Mice Being Submitted
*
We recommend at least 2 males
Male Mice Date Of Birth
*
YYYY slash MM slash DD
Genotype Of Male Mice
Number Of Female Mice Being Submitted
*
We recommend at least 10 females
Female Mice Date Of Birth
*
YYYY slash MM slash DD
Genotype Of Female Mice
Location Of Mice
*
Building and Room #
Health Status Of Mice
Sample Storage
*
MMRRC Storage: (If choosing storage at the MMRRC facility, there is a $200/year storage fee. This fee is pro-rated for the first year and invoiced at the time of cryopreservation.)
Self-Storage/Investigator Lab: (If choosing self-storage, straws will be held at the MMRRC facility until a QC in-vitro fertilization (IVF) is performed. Investigators are responsible for collecting their sample within 30 days of the date of QC, otherwise the sample will automatically be stored with the MMRRC and the investigating lab’s grant will be billed. If you leave the University without notifying us, we have the right to dispose of your samples.
All samples are preserved in Cassou insemination straws and must be stored directly in liquid nitrogen or in liquid nitrogen vapor. Samples cannot be stored in any other type of freezer.
QC Options
*
QC Thaw/IVF (no charge/included in cryo fee) - MMRRC at UNC performs a small IVF with QC straw of sperm to access quality of the sample. The fertilization rate is recorded and all embryos produced are cultured to blastocyst stage.
QC to pups ($1000) - MMRRC at UNC performs a small IVF with QC straw of sperm to access quality of the sample. The fertilization rate is recorded and embryos produced are transferred to a pseudo-pregnant female. If pups are born, the surrogate will be health tested after pups are weaned. Due to the strict health standards of our colony, every surrogate that births must be sent to RADIL for full health testing. Generally, it takes about 16 weeks until mice are ready to be transferred to your colony. If genotyping confirmation is necessary, we can take tail snips when the pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Additional Comments
Any additional information that you would like to include with this application
Sperm Cryorecovery General Disclaimer
Sperm must be shipped to the MMRRC at UNC directly to ensure proper handling of the aliquots. The MMRRC at UNC cannot make any guarantees that this procedure will be successful. Since the MMRRC at UNC did not cryopreserve the sperm, we cannot guarantee the quality of the sample. It is recommended to submit multiple aliquots of sperm for reanimation. We will attempt an IVF with 1 aliquot and save the others for back up. The thaw protocol must be submitted when the sperm aliquots are received. Without the proper thaw protocol, the MMRRC at UNC cannot perform an IVF. If the sperm has low fertilization and doesn’t provide enough embryos for a transfer, we can attempt the IVF again for a reduced fee ($1575) or perform ICSI (Intracytoplasmic Sperm Injection) for $3,000. If pups are born, the surrogate will be health tested after pups are weaned. Due to the strict health requirements of our colony, we require that every surrogate be sent to RADIL for health testing. The cost of health testing is included. Only after we have received the results can the pups be transferred to your colony (generally takes about 16 weeks). If genotyping confirmation is necessary, we can take tail snips when the pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Institution Sending The Sample
*
Is The Sending Institution A Commercial Vendor?
*
yes
no
Number Of Aliquots Submitted
*
Date To Be Shipped To MMRRC
*
YYYY slash MM slash DD
QC Data On Sample
*
Sperm Cryopreservation Protocol
Sperm Thaw Protocol
*
Health Status Of Sperm Donors
*
Additional Comments
Any additional information that you would like to include with this application
Embryo Cryorecovery General Disclaimer
Embryos must be shipped directly to the MMRRC at UNC to ensure proper handling of the samples. The MMRRC at UNC cannot make any guarantees that this procedure will be successful. Since the MMRRC at UNC did not cryopreserve the embryos, we cannot guarantee the quality of the sample. It is recommended to submit multiple aliquots of embryos for reanimation. The proper embryo thawing procedure must be provided to the MMRRC at UNC. The number of embryos straws thawed and transferred is up to MMRRC at UNC discretion. If the embryo transfer is unsuccessful and there are embryos left, the MMRRC at UNC can repeat the thaw and transfer for a reduced fee ($800). If pups are born, the surrogate will be health tested after pups are weaned. Due to the strict health requirements of our colony, we require that every surrogate be sent to RADIL for health testing. The cost of health testing is included. Only after we have received the results can the pups be transferred to your colony (generally takes about 16 weeks). If genotyping confirmation is necessary, we can take tail snips when the pups are 10 days old. Frozen tail snips will then be available for pick up by the investigator's lab.
Institution Sending The Sample
*
Is The Sending Institution A Commercial Vendor?
*
yes
no
Number Of Aliquots Submitted
*
Date To Be Shipped To MMRRC
*
YYYY slash MM slash DD
QC Data On Sample
*
Embryo Cryopreservation Protocol
Embryo Thaw Protocol
*
Genetic Cross To Make Embryos
Health Status Of Sperm Donors
Additional Comments
Any additional information that you would like to include with this application
Δ