Instrumentation

Browse by system: Confocal microscopes | Multiphoton system | Widefield microscope

Confocals

Coming soon:  new Zeiss LSM 880 with combined confocal and multiphoton imaging capabilities.

This state-of-the-art, upright confocal and multiphoton system includes a super-resolution module (Airyscan) and a module for faster image acquisition (Fast). Airyscan provides super-resolution imaging (1.5x improvement over confocal) and higher sensitivity. The Fast module improves image acquisition speed 4-fold while maintaining the super-resolution capability. with Airyscan and Fast modules. The new system will be also equipped with multiphoton acquisition module and will replace the Zeiss LSM 7MP.

 

Zeiss LSM 780
inverted confocal microscope with a motorized stage
Location: Rm. 8122 NRB


Z-stack acquisition | Mosaic imaging | 

Lasers Objectives


10x/0.30
EC Plan-Neofluar 
WD = 5.2 mm

20x/0.8
Plan-Apochromat 
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil)  DIC WD = 0.21 mm

40x/1.4
Plan-Apochromat (oil)  DIC WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil)  DIC
WD = 0.19 mm


Detectors

3 fluorescence
spectral detection: 2 flanking PMTs and the central 34-channel GaAsP detector with ~40% quantum efficiency
1 transmission
brightfield, DIC

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
561 nm
Diode laser
Cy3, RFP, Alexa 546, Alexa 561, dsRed2
594 nm
Diode laser
Alexa 594, mCherry
633 nm
Helium-Neon laser
Cy5, DRAQ5

 

Zeiss LSM 710
upright confocal microscope with a motorized stage (installed in December 2010)
Location: Rm. 8104 NRB


Z-stack acquisition | Mosaic imaging | FRAP

Lasers Objectives


10x/0.30
EC Plan-Neofluar 
WD = 5.2 mm

20x/0.8
Plan-Apochromat 
WD = 0.55 mm

40x/1.3
EC Plan-Neofluar (oil)  DIC WD = 0.21 mm

63x/1.40
Plan-Apochromat (oil)  DIC
WD = 0.19 mm


Detectors

3 fluorescence
spectral detection 
1 transmission
brightfield, DIC

405 nm
Diode Laser
DAPI, Hoechst 33258, Alexa 405
458/488/514 nm
Multiline Argon laser
458 nm: CFP
488 nm: FITC, Alexa 488, CY2, GFP, Bodipy, Fluo-4, Calcein
514 nm: YFP
543 nm
Helium-Neon laser
Cy3, RFP, Alexa 546, Alexa 561
633 nm
Helium-Neon laser
Cy5, DRAQ5

 

 


Multiphoton microscope

 


Widefield microscope