The primary goal of this core is to assist neuroscientists at UNC-Chapel Hill in their efforts to molecularly manipulate and study nervous system function. This Core is directed by Dr. JrGang Cheng, a pioneer in the field of BAC recombineering.
Our Facility has been in operation for more than 10 years and has made many complex BAC-derived constructs for use in mice. We have also generated constructs expressing Cre recombinase, Tet on/off trans-activators and optogenetic proteins to study neural circuitry function. We can also use recombineering to generate various mutations at the gene level including: exon swaps, duplications, truncations, deletions, point mutations and conditional gene modifications.
We offer the following services at an affordable cost to UNC neuroscientists:
1. Generate transgenic and gene-targeting constructs using Bacterial Artificial Chromosome (BAC) recombineering (fee-for-service).
2. Generate expression plasmids, recombinant adenoviral (rAAV) or other viral based plasmid constructs or any other type of plasmid construct (fee-for-service).
3. Create site-directed mutagenesis and epitope tagging on any plasmid.
4. Provide neuroscientists with access to a quantitative PCR (Q-PCR) machine (usage fee applies).
We provide free initial consultation in the design of transgenic or gene targeting constructs and plasmids.